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Method for recognizing carbapenems-resistant Escherichia coli by using pattern recognition technology

A technology of carbapenems and Escherichia coli, applied in the field of medical engineering, to achieve rapid and accurate identification, high resolution, and high sensitivity

Inactive Publication Date: 2019-06-14
CHINA UNIV OF MINING & TECH
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  • Claims
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Problems solved by technology

However, how to obtain complex system composition and molecular structure information efficiently and deeply from a large amount of Orbitrap-MS data is facing a huge challenge

Method used

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  • Method for recognizing carbapenems-resistant Escherichia coli by using pattern recognition technology
  • Method for recognizing carbapenems-resistant Escherichia coli by using pattern recognition technology
  • Method for recognizing carbapenems-resistant Escherichia coli by using pattern recognition technology

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Embodiment Construction

[0021] In order to deepen the understanding of the present invention, the present invention will be further described below in conjunction with the accompanying drawings and embodiments, which are only used to explain the present invention and do not limit the protection scope of the present invention.

[0022] Such as figure 1 As shown, the method for identifying carbapenem-resistant Escherichia coli using pattern recognition technology comprises the following steps:

[0023] 1) Source of E. coli:

[0024] All bacterial samples were collected and provided according to the requirements and in accordance with standardized and reasonable operating procedures, and were immediately frozen and stored at -80°C after collection. The numbers of the bacteria are: carbapenem-resistant Escherichia coli are numbered R1, R2, R3, R4, and sensitive Escherichia coli are numbered S1, S2, S3, S4.

[0025] 2) Reagents and instruments

[0026] Main Reagents and Drugs

[0027]

[0028] Main...

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Abstract

The invention discloses a method for recognizing carbapenems-resistant Escherichia coli by using the pattern recognition technology. The method for recognizing the carbapenems-resistant Escherichia coli by using the pattern recognition technology comprises the following steps: a, collecting Escherichia coli; b, extracting metabolites and polypeptides from the Escherichia coli; c, performing Orbitrap-MS analysis and detection; d, performing Orbitrap-MS data processing and analysis; and e, using an R language software and an own program to perform pattern recognition and analysis on the data. The powerful statistical capabilities of the R language software is used for processing large amounts of data obtained from the high-resolution Orbitrap-MS, and the pattern recognition and analysis is carried out on mass spectrometry data of carbapenems medicine-resistant Escherichia coli strains and sensitive Escherichia coli strains, so that the fast and accurate recognition of the carbapenems-resistant Escherichia coli strains and the sensitive Escherichia coli strains is realized. Meanwhile, the compounds with significant differences at the statistics are found, so that a theoretical basis for studying the differences between the carbapenems-resistant Escherichia coli strains and the sensitive Escherichia coli strains and elucidating the resistance mechanisms of Escherichia coli is provided.

Description

technical field [0001] The invention relates to the technical field of medical engineering, in particular to a method for identifying carbapenem-resistant Escherichia coli using pattern recognition technology. Background technique [0002] Enterobacteriaceae are common clinical pathogens, mainly including Escherichia coli and Klebsiella pneumoniae. At present, carbapenem antibiotics such as imipenem used clinically are considered to be the most effective antibacterial drugs for the treatment of this drug-resistant bacteria, and are also considered to be the ultimate method of treatment. However, with the use of such antibiotics, the emergence of carbapenem-resistant Enterobacteriaceae has greatly limited the use of such antibiotics. Among them, the resistance rate of Escherichia coli resistant to carbapenem antibiotics has also risen from zero in the early years to 1%, resulting in great difficulties in clinical treatment. [0003] The methods for identifying carbapenem-re...

Claims

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Application Information

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IPC IPC(8): G01N27/62G16B40/00G16B20/00
Inventor 樊星李蓓顾兵周愿赵云鹏王瑞玉魏贤勇
Owner CHINA UNIV OF MINING & TECH
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