Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing human umbilical cord mesenchymal stem cell exosomes

A technology for stem cells and exosomes, applied in the field of preparation of human umbilical cord mesenchymal stem cell exosomes, can solve the problems of low efficiency of immunomagnetic beads method and chromatography method, unfavorable large-scale production, complicated operation, etc. The bacteria are free of mycoplasma contamination, easy to mass-produce, and the effect of high product safety

Pending Publication Date: 2019-06-14
JINAN PANSHENG BIOTECH
View PDF3 Cites 25 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The sample obtained by density gradient centrifugation is relatively pure, but it is time-consuming and complicated to operate, which is not conducive to large-scale production; the immunomagnetic bead method and chromatography also have problems of low efficiency and difficulty in large-scale production; the kit method is simple to operate, but The cost is relatively high, and the purity of isolated exosomes is low

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing human umbilical cord mesenchymal stem cell exosomes
  • Method for preparing human umbilical cord mesenchymal stem cell exosomes
  • Method for preparing human umbilical cord mesenchymal stem cell exosomes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0037] The present invention provides a method for preparing exosomes derived from umbilical cord mesenchymal stem cells, comprising the following steps:

[0038] 1. Culture and identification of umbilical cord mesenchymal stem cells

[0039] 1.1 Isolation and culture: Collect the umbilical cord of full-term cesarean section neonates under sterile conditions and process it within 4 hours. Cut the umbilical cord with scissors into small sections of about 2-3 cm / , and wash the umbilical cord repeatedly with saline containing penicillin-streptomycin to remove surface blood stains. Remove the three blood vessels and amniotic membrane, peel off the Huatong glue, and cut the Huatong glue to about 1mm with scissors 2 small pieces, spread evenly on T75cm 2 In a culture bottle (when laying the bottle, try to distribute the tissue pieces evenly, leave a gap in the middle, do not cut out air bubbles, which is not conducive to cell climbing out), place at 37°C, 5% CO 2 Cultivate in an ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for preparing human umbilical cord mesenchymal stem cell exosomes. The method uses a newborn umbilical cord as a source of umbilical cord mesenchymal stem cells, usesa medium prepared by a fetal calf serum or a serum substitute in which exosome carried by itself is removedfor culturing, collects a supernatant, and further separates and extracts the exosomesby differential centrifugation, the purity is high, and the source of the umbilical cord mesenchymal stem cell exosomes is ensured; the exosomes is prepared by the differential centrifugation, the operationis simple, and the method is suitable for large-scale production; during the whole process, sterility is guaranteed, the contamination ofmycoplasma is prevented, and the product safety is higher.

Description

technical field [0001] The invention relates to a method for preparing human umbilical cord mesenchymal stem cell exosomes, belonging to the field of cell culture. Background technique [0002] Umbilical cord mesenchymal stem cells are mesenchymal stem cells derived from umbilical cord Huatong jelly tissue. It has a wide range of sources, low immunogenicity, and has multiple functions such as differentiation, immune regulation, and hematopoietic support. It has become the seed cell of cell therapy. It has good clinical efficacy in many diseases including diabetes, nervous system disease, liver disease, cardiovascular disease, autoimmune disease and other diseases, but its mechanism of action is still unclear. It was initially believed that umbilical cord mesenchymal stem cells would homing to the damaged site after transplantation into the human body for cell self-renewal and directional differentiation. The limited number of target cells does not adequately account for the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N5/0775
Inventor 陈莉邢志青王红丽田晓婷朱继玲周健关美月
Owner JINAN PANSHENG BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com