Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method and application of coumarin glycoside compounds

A technology of coumarin glycosides and compounds, applied in the field of pesticides, to achieve significant economic and social benefits and good application value

Active Publication Date: 2019-06-14
HENAN UNIV OF CHINESE MEDICINE
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The coumarin glycoside compounds involved in the present invention and their biological activity have not been reported in patents or literature so far

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method and application of coumarin glycoside compounds
  • Preparation method and application of coumarin glycoside compounds
  • Preparation method and application of coumarin glycoside compounds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] In the specific implementation of the present invention, a kind of coumarin glycoside compound, the compound is made of 20kg of small bark bone as a raw material, and is extracted 3 times by heating at 90°C with ethanol with 2-5 times the weight of the raw material and a volume concentration of 95%. The extraction time is 1 hour, and ethanol is recovered under reduced pressure to obtain extract-like ethanol extract, which is suspended in 4.8L of distilled water, and extracted 3 times with petroleum ether, ethyl acetate, and n-butanol successively, 4.8L each time, The time is 1.5 hours; the n-butanol extraction part is separated by AB-8 macroporous adsorption resin column chromatography, and the volume ratio is 0:100, 30:70, 95:5 ethanol-water mixed solvent for gradient elution, each One gradient uses 5.0L eluent, the flow rate is 10mLmin -1 , the water eluent was discarded, and the eluate fractions with a volume ratio of 30:70 and 95:5 ethanol-water were collected respe...

Embodiment 2

[0014] In the specific implementation of the present invention, a kind of coumarin glycoside compound, with 15kg of small barb bone as raw material, is 3 times of raw material weight, and volume concentration is 75% ethanol heating 95 ℃ of reflux extraction 3 times, and each extraction time is For 1 hour, ethanol was recovered under reduced pressure to obtain extract-like ethanol extract, which was suspended in 3.6L of distilled water, and extracted three times with petroleum ether, ethyl acetate, and n-butanol in sequence, 3.6L each time, for 1.5 hours ; The n-butanol extraction part is separated by AB-8 macroporous adsorption resin column chromatography, and the volume ratio is 0:100, 30:70, 95:5 ethanol-water mixed solvent for gradient elution, and each gradient uses 3.8 L eluent, the flow rate is 5mLmin -1 , the water eluent was discarded, and the eluate fractions with a volume ratio of 30:70 and 95:5 ethanol-water were collected respectively, concentrated and dried under ...

Embodiment 3

[0016] In the specific implementation of the present invention, a kind of coumarin glucoside compound, with 18kg of small barb bone as raw material, with 4 times of raw material weight, volume concentration is 85% ethanol heating 92 ℃ of reflux extractions 3 times, each extraction time is After 1 hour, ethanol was recovered under reduced pressure to obtain extract-like ethanol extract, which was suspended in 4.5L of distilled water, and extracted three times with petroleum ether, ethyl acetate, and n-butanol in sequence, 4.5L each time, for 1.5 hours ; The n-butanol extraction part is separated by AB-8 macroporous adsorption resin column chromatography, and the volume ratio is 0:100, 30:70, 95:5 ethanol-water mixed solvent for gradient elution, and each gradient uses 4.5 L eluent, the flow rate is 8mLmin -1 , the water eluent was discarded, and the eluate fractions with a volume ratio of 30:70 and 95:5 ethanol-water were collected respectively, concentrated and dried under red...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Login to View More

Abstract

The invention relates to a preparation method and application of coumarin glycoside compounds. The preparation method can effectively achieve the technical effects of preparing the novel coumarin glycoside compounds and achieving the preparation of antibacterial drugs. The compounds are genglycoside C (I), genglycoside E (II) and genglycoside F (III) which are separated from a medicinal material of common gendarussa herb. The prepared novel coumarin glycoside compounds genglycoside C (I), genglycoside E (II) and genglycoside F (III) which have antimicrobial activity serve as the only active ingredients to be effectively used for preparing pesticides resisting agrobacterium tumefaciens, open up a new path for antibacterial pesticides and the medicinal value and commercial value of the common gendarussa herb, and have higher practical application value and significant economic and social benefits.

Description

technical field [0001] The invention relates to pesticides, in particular to a preparation method and application of coumarin glycoside compounds. Background technique [0002] The widespread use of chemically synthesized pesticides not only pollutes the environment, but also accelerates the emergence of drug-resistant bacteria, and even multidrug-resistant bacteria. Botanical pesticides have different structural characteristics. Due to the complexity and diversity of the structure, they have incomparable advantages in the field of pesticides, such as fast biodegradation, low toxicity, not easy to produce resistance, and no pollution to the environment. It has attracted more and more attention from researchers. Therefore, searching for new pesticides with antibacterial activity with high efficiency and low toxicity from Chinese herbal medicines, and developing antibacterial pesticides with strong selectivity and low toxic and side effects are the most urgent problems for ph...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07H17/065C07H1/08A01P1/00
Inventor 孙彦君高美玲陈豪杰冯卫生李孟杨静万焱王俊敏张艳丽韩瑞杰赵晨
Owner HENAN UNIV OF CHINESE MEDICINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com