Method for grafting Chinese rose plants by using Robinia plants as rootstocks
A plant and acacia technology, applied in the field of biological tissue culture, can solve the problems of increasing the cost of grafting roses, roses, and roses, no materials are available for high-stem grafting of roses, and the extinction of S. , the effect of easy acquisition
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Embodiment 1
[0019] A method for grafting a rose plant as a rootstock with a locust plant, comprising the following steps:
[0020] A: Cultivation of Robinia pseudoacacia: The stem segment of Robinia pseudoacacia with buds is used as an explant, and the explant is soaked in water for 10 minutes, and then sterilized with 80% ethanol. After 1 minute, the Rinse the explants repeatedly with sterile water and insert into the primary medium for culture; after 20-30 days, cut off the buds that sprout from the stem segments and insert them into the proliferation medium. After 10-20 days, when the seedlings grow to 3.0 -5.5cm, insert into rooting medium and cultivate;
[0021] B: Grafting: Select Robinia pseudoacacia as rootstock, and the thickness at the fixed stem height during grafting reaches 0.5cm-0.8cm, select disease-free and sturdy rose branches for scion, and remove terminal buds before picking ears , use a grafting knife to cut the shield-shaped buds and peel off the bud skins, soak them...
Embodiment 2
[0024] A method for grafting a rose plant as a rootstock with a locust plant, comprising the following steps:
[0025] A: Cultivation of Robinia pseudoacacia: The stem segment of Robinia pseudoacacia with buds is used as an explant, and the explant is soaked in water for 10 minutes, and then sterilized with 80% ethanol. After 1 minute, the Rinse the explants repeatedly with sterile water and insert into the primary medium for culture; after 20-30 days, cut off the buds that sprout from the stem segments and insert them into the proliferation medium. After 10-20 days, when the seedlings grow to 3.0 -5.5cm, insert into rooting medium and cultivate;
[0026] B: Grafting: Select Robinia pseudoacacia as rootstock, and the thickness at the fixed stem height during grafting reaches 0.5cm-0.8cm, select disease-free and sturdy rose branches for scion, and remove terminal buds before picking ears , split the Robinia pseudoacacia along the top with a machete, cut the front and back side...
Embodiment 3
[0029] A method for grafting a rose plant as a rootstock with a locust plant, comprising the following steps:
[0030] A: Cultivation of Robinia pseudoacacia: The stem segment of Robinia pseudoacacia with buds is used as an explant, and the explant is soaked in water for 10 minutes, and then sterilized with 80% ethanol. After 1 minute, the Rinse the explants repeatedly with sterile water and insert into the primary medium for culture; after 20-30 days, cut off the buds that sprout from the stem segments and insert them into the proliferation medium. After 10-20 days, when the seedlings grow to 3.0 -5.5cm, insert into rooting medium and cultivate;
[0031] B: Grafting: Select Robinia pseudoacacia as rootstock, and the thickness at the fixed stem height during grafting reaches 0.5cm-0.8cm, select disease-free and sturdy rose branches for scion, and remove terminal buds before picking ears , Cut the bark of the locust tree vertically along the top with a grafting knife, peel the...
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