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Multi-target magnetic immunochemiluminescence micro-fluidic chip detection platform and method

A microfluidic chip and chemiluminescence technology, applied in chemical instruments and methods, chemiluminescence/bioluminescence, and analysis through chemical reactions of materials, can solve the problem of high reagent consumption, insufficient mixing, and simultaneous detection of multiple targets Limitation and other problems, to achieve the effect of convenient operation, fast detection and low cost

Active Publication Date: 2019-06-11
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The invention solves the problems of high reagent consumption, insufficient mixing, and limited simultaneous detection of multiple targets in the prior art platform, and provides a multi-target magnetic immunochemiluminescence microfluidic chip detection platform and method

Method used

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  • Multi-target magnetic immunochemiluminescence micro-fluidic chip detection platform and method
  • Multi-target magnetic immunochemiluminescence micro-fluidic chip detection platform and method
  • Multi-target magnetic immunochemiluminescence micro-fluidic chip detection platform and method

Examples

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Embodiment 1

[0032] The chemiluminescent molecule in this example is HRP enzyme, and the luminescent system is luminol chemiluminescent system. See the attached schematic diagram for details. image 3 ;

[0033] (1) if image 3 As shown, there are three target molecules in the sample. When the sample is injected through the sample hole, it flows through the liquid flow connection channel to reconstitute the antibody in the coating area; the first target reacts with the corresponding magnetic-labeled antibody and enzyme-labeled antibody through the mixing channel. , forming the first target sandwich complex; the particle size of the magnetic beads is 10-1000nm, preferably 500nm;

[0034] (2) When the first target sandwich complex flows through the capture area, due to the magnetic force at the position of the capture area, the first target sandwich complex is captured and fixed in this area,

[0035] (3) The remaining samples continue to flow through the next antigen immobilization area, ...

Embodiment 2

[0041] A multi-target magnetic immunochemiluminescence microfluidic chip detection platform, its schematic diagram is as follows figure 1 As mentioned above, the detection platform includes a sampling hole 1, a liquid flow connection channel 2, a target detection area 3, a waste liquid port 4, a cleaning liquid pool 5, and a luminescent liquid pool 6; there are at least two target detection areas 3; The target detection area 3 includes a sequentially connected coating area 7, a mixing area 8 and a capture area 9; the liquid flow connection channel 2 is used to transport the sample injected in the sample hole 1 to the target detection area 3; different The coating area 7 in the target detection area 3 contains antibody A and antibody B that can specifically bind to different targets in the sample at the same time, and the antibody A is a chemiluminescence molecule or an enzyme-labeled antibody that can make the substrate emit light , the antibody B is a magnetic bead-labeled an...

Embodiment 3

[0043] A multi-target magnetic immunochemiluminescence microfluidic chip detection platform, its schematic diagram is as follows figure 2 As mentioned above, the detection platform includes a sampling hole 1, a liquid flow connection channel 2, a target detection area 3, a waste liquid port 4, a cleaning liquid pool 5, and a luminescent liquid pool 6; there are at least two target detection areas 3; The target detection area 3 includes a sequentially connected coating area 7, a mixing area 8 and a capture area 9; the liquid flow connection channel 2 is used to transport the sample injected in the sample hole 1 to the target detection area 3; different The coating area 7 in the target detection area 3 contains antibody A and antibody B that can specifically bind to different targets in the sample at the same time, and the antibody A is a chemiluminescence molecule or an enzyme-labeled antibody that can make the substrate emit light , the antibody B is a magnetic bead-labeled a...

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Abstract

The invention discloses a multi-target magnetic immunochemiluminescence micro-fluidic chip detection platform and method, and belongs to the field of rapid diagnosis in vitro. The detection platform comprises a sample introduction hole, a liquid flow connection channel, a target detection area, a liquid waste port, a cleaning liquid pool and a luminescent liquid pool; the target detection area comprises a coating area, a mixing area and capture areas, which are connected in sequence; the detection method comprises the following steps: injecting a sample into the sample introduction hole, so that the sample flows by the liquid flow connection channel, redissolves an antibody in the coating area and forms a target compound to be captured, causing the remaining sample to flow by the downstream coating area to form the target compound, capturing the target compound, until target molecules in the sample are captured separately; and then, removing free non-target molecules by using cleaningliquid, releasing the luminescent liquid, causing the same to flow by the capture areas in sequence, and performing signal collection. The platform integrates sample introduction, magnetic absorption,high-efficiency capture and separation and fast detection, and can detect multiple targets on one chip at the same time, and the multi-target magnetic immunochemiluminescence micro-fluidic chip detection platform has the characteristics of convenient operation, fast detection, low cost, etc.

Description

technical field [0001] The invention belongs to the field of in vitro rapid diagnosis, and in particular relates to a multi-target magnetic immunochemiluminescence microfluidic chip detection platform and method. Background technique [0002] In vitro diagnostics (IVD) is a diagnostic method that tests human samples such as blood and urine outside the body to diagnose diseases or bodily functions. At present, there are two main development trends for in vitro diagnostics (IVD): one is automation and integration, that is, the use of fully automated and highly sensitive large-scale instruments and equipment in the central laboratory of a large hospital to achieve high-precision disease analysis and diagnosis ; Another kind of miniaturization and bedside, that is, to realize rapid analysis and diagnosis on the spot through small and simple handheld devices. [0003] Chemiluminescence immunoassay is a combination of highly sensitive chemiluminescent assay technology and highly ...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/535G01N21/76B01L3/00
Inventor 刘笔锋陈鹏
Owner HUAZHONG UNIV OF SCI & TECH
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