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Serum pancreatic cancer exosome specific protein and re-verification process thereof

A specific protein and verification process technology, applied in the field of serum pancreatic cancer exosome specific protein and its re-validation process, can solve the problems of uneven quality and difficulty in obtaining antibodies

Inactive Publication Date: 2019-06-07
AFFILIATED HOSPITAL OF NANTONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, various proteins require corresponding antibodies, and antibodies are difficult to obtain and the quality is uneven, which is one of the technical obstacles faced by research

Method used

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  • Serum pancreatic cancer exosome specific protein and re-verification process thereof
  • Serum pancreatic cancer exosome specific protein and re-verification process thereof
  • Serum pancreatic cancer exosome specific protein and re-verification process thereof

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Embodiment Construction

[0016] In order to make the technical problems, technical solutions and advantages to be solved by the present invention clearer, the following will describe in detail with reference to the drawings and specific embodiments.

[0017] In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", " The orientation or positional relationship indicated by "front", "rear", etc. is based on the orientation or positional relationship shown in the drawings, which is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the referred device or element must have a specific orientation, are constructed and operate in a particular orientation and therefore are not to be construed as limiting the invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only, and shou...

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Abstract

The invention provides a serum pancreatic cancer exosome specific protein, which comprises a protein ADAM10, a protein CFHR1, a protein APOA4, a protein testicular tissue protein Li 70, a protein HEL-S-78p, a protein PGLYRP2, a protein IGFALS, a protein BCHE, a protein ANPEP, a protein LBP, a protein C9, a protein PZP and a protein ANXA11. The invention further provides a re-verification process of the serum pancreatic cancer exosome specific protein, which comprises the steps of S1, separating the exosome-free serum and the exosome in a serum sample; S2, preparing an exosome PBS solution by adopting the exosome separated in the step S1; S3, checking the ultra-micro structure of the exosome through a transmission electron microscope; and S4, detecting the expression of some maker proteinson the surface of the exosome by through Western blot analysis. The serum pancreatic cancer exosome specific protein of the invention can be used for preparing related product for pancreatic cancer diagnosis, the re-verification process of the serum pancreatic cancer exosome specific protein has the advantages of absolute specificity, high throughput, no interference and ability of recognizing macromolecular isomers and small molecules.

Description

technical field [0001] The invention belongs to the technical field of a verification method for a serum pancreatic cancer exosome-specific protein, and in particular relates to a serum pancreatic cancer exosome-specific protein and a re-verification process thereof. Background technique [0002] At present, the verification method of serum pancreatic cancer exosome-specific proteins is the method of detecting exosomes with specific antibodies corresponding to the proteins. The Western Blot method is commonly used for cells and tissues: protein samples separated by PAGE (polyacrylamide gel electrophoresis) are transferred to a solid phase support (such as nitrocellulose membrane), and the protein or polypeptide on the solid phase support is used as an antigen. Immunoreact with the corresponding antibody, then react with the enzyme or isotope-labeled secondary antibody, and detect the protein component expressed by the specific target gene separated by electrophoresis through...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 肖明兵陈晓阳季洁瞿利帅刘金霞焦钰洁江枫钱坤艳纪易斐
Owner AFFILIATED HOSPITAL OF NANTONG UNIV
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