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Py-M-COF, electrochemical sensor thereof, and application of electrochemical sensor

An electrochemical and sensor technology, applied in the application field of new covalent organic framework electrochemical sensors and their detection of antibiotics, can solve the problems of application limitations, poor electrochemical activity, immature research on electrochemical sensors, etc., and achieve high biological affinity Harmony, promotion of charge transport, and improvement of detection sensitivity

Active Publication Date: 2019-06-07
郑州赫诺瑞信息科技有限公司
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  • Abstract
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  • Application Information

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Problems solved by technology

However, most COFs have poor electrochemical activity, and their applications as electrochemical biosensor platforms are limited.
In particular, research on COF-based electrochemical sensors is immature

Method used

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  • Py-M-COF, electrochemical sensor thereof, and application of electrochemical sensor
  • Py-M-COF, electrochemical sensor thereof, and application of electrochemical sensor
  • Py-M-COF, electrochemical sensor thereof, and application of electrochemical sensor

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Embodiment Construction

[0025] The invention will be further described below in conjunction with specific embodiments.

[0026] Introduction to the source of materials: 1,3,6,8-Tetrakis(4-formylphenyl)pyrene (TFPPy), melamine and dimethyl sulfoxide (DMSO) were purchased from Aladdin Reagent Co., Ltd. (Shanghai, China). Enrofloxacin (ENR), aptamer, tetracycline, kanamycin (Kana), tobramycin (TOB), streptomycin, oxytetracycline (OTC) and human serum were purchased from Solarbio Bioengineering Co., Ltd. ( Beijing). KH2PO4, Na2HPO4·12H2O, KCl, NaCl, K3[Fe(CN)6] and K4[Fe(CN)6]·H2O were purchased from Sinopharm Chemical Reagent Co., Ltd. (Beijing). All chemical reagents used were of analytical grade and used without further purification. All solutions were prepared with ultrapure water (≥18.2 MΩ·cm). The targeting aptamer sequence is as follows: ENR targeting aptamer (AptENR): 5'-CCC ATC AGG-GTG-CTA GGC-TACAC GGT TCG GCT CTC TGA GCC CGG GTT ATTTCA GGG-GGA-3'.

[0027] 1. Preparation and characterization...

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Abstract

The invention discloses a novel covalent organic framework Py-M-COF, an electrochemical sensor thereof, and an application of the electrochemical sensor. 1,3,6,8-tetrakis(4-formylphenyl)pyrene is dissolved in dimethyl sulfoxide, then melamine is added, the obtained solution undergoes hot bath treatment at 180 DEG C for 3 d, and a product obtained after the hot bath treatment is repeatedly washed with tetrahydrofuran and ethanol, and then is dried in a 60 DEG C oven to obtain the Py-M-COF. The novel covalent organic framework Py-M-COF has a high specific surface area and a porous framework, soa large surface interface is provided for biomolecule fixation; an aptamer chain can be adhered to a COF matrix through the action of pi-pi accumulation and hydrogen bonds, and an amino end functionalgroup and a negatively charged aptamer can be combined by electrostatic interaction; the extended pi conjugated framework structure can promote charge transport and improve the detection sensitivity;and the Py-M-COF has high bioaffinity to the aptamer chain, is ultrasensitive and selective for the detection of trace antibiotics, has a lowest detection limit for an antibiotic enrofloxacin of 6.068 fg.ml<-1>.

Description

technical field [0001] The invention relates to the technical field of electrochemical sensors, in particular to a novel covalent organic framework electrochemical sensor and its application for detecting antibiotics. Background technique [0002] In recent years, antibiotics have been widely used in medical and health, food industry, animal husbandry and other fields to treat diseases and promote animal growth. In my country, the amount of antibiotics used is astonishing, accounting for about half of the global amount, and a large amount of them are discharged into the water and soil environment, seriously endangering human health and the ecological environment. CCTV news revealed that more than 1,000 children in Jiangsu, Zhejiang and Shanghai were tested for urine, and nearly 60% of the children were found to have antibiotics in their urine. Among them, 1 / 4 of the children had more than 2 types of antibiotics detected in their urine, and some urine samples could even detec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G83/00G01N27/30G01N27/327
Inventor 张治红赵银霞何领好刘永康王明花胡梦瑶崔冰冰宋英攀
Owner 郑州赫诺瑞信息科技有限公司
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