Freezing protective solution of human placenta amnion and chorion, and preparation method of freezing protective solution

Abstract

The invention provides a freezing protective solution of human placenta amnion and chorion, and a preparation method of the freezing protective solution. The freezing protective solution is mainly composed of dimethylsulfoxide (DMSO), sodium dodecyl amino propionate, carbomer microspheres and lecithin according to certain weight parts. According to the freezing protective solution, a mixture of the sodium dodecyl amino propionate, the carbomer microspheres and the lecithin is added into the DMSO, so that the storing time of the human placenta amnion and chorion by using the freezing protectivesolution is obviously prolonged, and cell viability is preserved for a long time.

Description

technical field [0001] The invention belongs to the technical field of stem cell cryopreservation, and in particular relates to a cryoprotectant solution for human placental amnion and chorion and a preparation method thereof. Background technique [0002] Amnion and chorion are the two main components of the human placenta. The amniotic membrane is the innermost layer of the placenta, which has certain elasticity and pluripotency, and will not induce immune rejection after transplantation. It can secrete neurotrophic factors and can also be used as a basement membrane for ophthalmic surgery; and The chorion also contains abundant stem cells with multi-differentiation potential, which can be applied to tissue and organ damage repair and transplantation caused by aging and disease. [0003] Although amnion and chorion have a wide range of applications, their cryopreservation has become a bottleneck in application. In the prior art, the preservation solution used to preserve...

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Problems solved by technology

[0003] Although amnion and chorion have a wide range of applications, their cryopreservation has become the bottleneck of application

In the prior art, the preservation solution used to preserve amniotic membrane and chorion is a cryoprotectant solution composed of fetal bovine serum, dimethyl sulfoxide and dextran 40. The time for the preservation solution to maintain cell viability is relatively short, which cannot meet the long-term clinical requirements. The need for preservation, and the fetal bovine serum used in the protection solution is expensive, and there are significant differences in different production batches and quality of different manufacturers, which will have a significant impact on the quality of the entire cryoprotection solution, thereby affecting cryopreservation cell recovery rate

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