Single-chain antibody targeting CD19, chimeric antigen receptor T cell, preparation method and application thereof
A chimeric antigen receptor and single-chain antibody technology, applied in the field of medical biology, can solve the problem of failing to kill tumor cells, and achieve the effects of promoting amplification, high infection efficiency, and lasting in vivo maintenance ability.
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[0077] A method for preparing chimeric antigen receptor T cells targeting CD19, comprising the following steps:
[0078] (1) Prepare the gene sequence of chimeric antigen receptor CAR-CD19 targeting CD19
[0079]Prepare the coding genes of the signal peptide, single-chain antibody targeting CD19, CD8α hinge region, CD8 transmembrane region, 4-1BB signal region and CD3ζ signal region respectively, the coding gene sequence of the signal peptide is as SEQ ID NO: 15 As shown, the coding gene sequence of the single-chain antibody targeting CD19 is shown in SEQ ID NO: 2, the coding gene sequence of the CD8α hinge region is shown in SEQ ID NO: 7, and the coding gene sequence of the CD8 transmembrane region The gene sequence is shown in SEQ ID NO: 9, the coding gene sequence of the 4-1BB signal region is shown in SEQ ID NO: 11, and the coding gene sequence of the CD3ζ signal region is shown in SEQ ID NO: 13.
[0080] The coding gene sequences of the above-mentioned signal peptide, si...
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