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A kind of microbial immobilization agent and preparation method thereof for improving tea garden soil available nitrogen

A microbial immobilization and soil technology, which is applied in the direction of fixing on or in inorganic carriers, fixing on/in organic carriers, organic fertilizers, etc., can solve the problems of phagocytosis, easy loss of microorganisms, and nitrogen-limiting transformation bacteria. Application effects and other issues

Inactive Publication Date: 2021-04-09
TEA RES INST ANHUI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in practical application, since the tea garden soil environment is rich in high active aluminum, it will cause growth stress to most nitrogen transforming bacteria, and at the same time, the applied microorganisms are also easy to be lost or swallowed by other microorganisms, which are serious limitations The practical application effect of nitrogen transforming bacteria

Method used

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  • A kind of microbial immobilization agent and preparation method thereof for improving tea garden soil available nitrogen
  • A kind of microbial immobilization agent and preparation method thereof for improving tea garden soil available nitrogen
  • A kind of microbial immobilization agent and preparation method thereof for improving tea garden soil available nitrogen

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Effect test

Embodiment 1

[0025] The preparation steps of the microorganism immobilizing agent used in embodiment 1 are as follows:

[0026] 1) Acquisition of nitrogen-converting bacteria: Take 10 g of soil sample and put it into an Erlenmeyer flask, add 90 mL of sterile water to the Erlenmeyer flask for dilution, and the sterile water contains 100 mM A l3+ , and shake overnight to obtain a soil suspension. Use a 1mL sterile pipette to draw the above soil suspension into a 9mL sterile water test tube for dilution, and dilute in this way to obtain 10 2 、10 3 and 10 4 double dilution. Take 100 μL of the above 10 2 、10 3 and 10 4 Spread the two-fold dilution on casein solid medium, culture at 28°C for 3 days, isolate and purify the strains with better growth, transfer them to Asobi nitrogen-free medium, and culture at 28°C for 3 days, select the strains with better growth strains are nitrogen transforming bacteria.

[0027] 2) Cultivation of nitrogen-converting bacteria: inoculate the nitrogen-con...

Embodiment 2

[0040] The preparation steps of the microorganism immobilizing agent used in embodiment 2 are as follows:

[0041] 1) Acquisition of nitrogen-converting bacteria: Take 10 g of soil sample and put it into an Erlenmeyer flask, add 90 mL of sterile water to the Erlenmeyer flask for dilution, and the sterile water contains 100 mM A l3+ , and shake overnight to obtain a soil suspension. Use a 1mL sterile pipette to draw the above soil suspension into a 9mL sterile water test tube for dilution, and dilute in this way to obtain 10 2 、10 3 and 10 4 double dilution. Take 100 μL of the above 10 2 、10 3 and 10 4 Spread the two-fold dilution on casein solid medium, culture at 28°C for 4 days, isolate and purify the strains that grow better, transfer them to Asobi nitrogen-free medium, and culture at 28°C for 4 days, select the strain that grows better strains are nitrogen transforming bacteria.

[0042] 2) Cultivation of nitrogen-converting bacteria: inoculate the nitrogen-convert...

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Abstract

The invention belongs to the technical field of agricultural intensive production, and in particular relates to a microorganism immobilizing agent capable of increasing available nitrogen in tea garden soil and a preparation method thereof. The microbial immobilization agent of the present invention includes nitrogen-transforming bacteria, adsorption materials and embedding agents, and the mass proportion of the three is 8:1:400 in sequence, and the microbial immobilization agent contains 1.0×10 viable bacteria 7 -1.0×10 8 cfu / g. The microbial immobilizer can effectively convert the nitrogen in the soil and even the air into available nitrogen that tea trees can absorb, thereby effectively reducing the amount of chemical fertilizers used in tea gardens and ensuring the quality of tea. Another object of the present invention is to provide a preparation method of the above-mentioned microorganism immobilizing agent to realize the precise preparation of the above-mentioned microorganism immobilizing agent with convenience and high efficiency; the preparation method includes the acquisition of nitrogen-transforming bacteria, nitrogen conversion Bacteria cultivation, preparation of embedding agent and preparation of microorganism immobilization agent.

Description

technical field [0001] The invention belongs to the technical field of agricultural intensive production, and in particular relates to a microorganism immobilizing agent capable of increasing available nitrogen in tea garden soil and a preparation method thereof. Background technique [0002] Tea tree is one of the main leaf-using economic crops in my country, and the annual leaf harvesting will take away a large amount of nitrogen-containing nutrients. Due to the low nutrient content of organic fertilizers, there are few effective nutrients in the short term, and it is difficult to meet the needs of crop growth in the current season. Therefore, in order to maintain production, tea farmers can only apply a large amount of chemical nitrogen fertilizer to the tea garden every year. However, the long-term application of chemical fertilizers in tea gardens can easily cause soil compaction, increase in soil-borne diseases, decline in tea quality, and aggravation of agricultural ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/14C12N11/10C12N11/04C05G3/80C05F11/08
Inventor 罗毅王烨军孙宇龙夏先江张永利苏有健廖珺
Owner TEA RES INST ANHUI ACAD OF AGRI SCI
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