Test strip for testing residual diazepam

A technology of test strips and stability, which is applied in the field of colloidal gold test strips for the detection of stable drug residues. It can solve the problems of complex processing, environmental pollution, and long detection time, and achieve the effects of simple operation, high sensitivity, and short detection time.

Inactive Publication Date: 2019-05-28
贵州勤邦食品安全科学技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] For the detection of diazepam residues, the country formulates corresponding testing standards, such as "GB 29688-2013 Food Safety National Standard for the Determination of Diazepam Residues in Feeds by Liquid Chromatography-Tandem Mass Spectrometry", and the current residue detection methods are liq

Method used

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  • Test strip for testing residual diazepam
  • Test strip for testing residual diazepam
  • Test strip for testing residual diazepam

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 Preparation of Stability Detection Test Paper Card

[0046] 1. Synthesis and identification of diazepam hapten and diazepam hapten-carrier protein conjugate

[0047] (1) Synthesis of stable hapten

[0048] Weigh 5g of diazepam raw material, dissolve 5g of diazepam and an appropriate amount of magnesium in an appropriate amount of tetrahydrofuran, react under nitrogen protection for 2-3h, add ethyl tetrabromobutyrate dropwise, and react for another 2-3h;

[0049] TLC thin-layer plate detection reaction, until there is no raw material or the raw material point is very shallow, the reaction is terminated;

[0050]Silica gel column purification, concentration to obtain the product, which is 4-bromobutyric acid ethyl ester derivative, hydrolyzed to obtain stable hapten;

[0051] The reaction technical route is as follows:

[0052]

[0053]

[0054] The molecular structural formula of the hapten obtained by the technical route is shown in Formula I:

[005...

Embodiment 2

[0085] Example 2 Detection of Stability Residues in Samples

[0086] 1. Sample pretreatment

[0087] (1) Animal tissue pretreatment

[0088] Weigh 2.0±0.05g of homogenized tissue samples into a centrifuge tube, add 2mL of deionized water containing 10% methanol, and cap the bottle tightly. Place the centrifuge tube containing the sample in a water bath at 80°C for 10 minutes, absorb more than three drops of the solution and pour it into a 1.5mL centrifuge tube. If there is obvious yellow turbidity, please centrifuge, and then use the supernatant as the sample solution to be tested.

[0089] (2) Pretreatment of urine samples

[0090] Put a small amount of urine sample in a centrifuge tube, add 1mL of deionized water, and mix it as the sample solution to be tested.

[0091] 2, detect with the test paper card of the present invention

[0092] Use a dropper to drop 3 drops of sample into the hole of the reagent card, and observe the result after 5-8 minutes. If it exceeds 10...

Embodiment 3

[0095] Example 3 Example of sample detection

[0096] Take 20 samples each of pork, chicken, and urine and 20 samples of various negative samples with known stable drug residual concentration greater than 5ng / g, and calculate the negative and positive rates. The results are shown in Table 1-Table 3.

[0097] Table 1 Negative and positive rate detection of pork samples

[0098] batch

[0099] Table 2 Detection of negative and positive rate of chicken samples

[0100] batch

[0101] Table 3 Negative and positive rate detection of urine samples

[0102] batch

[0103] The results show that there are 20 positive samples and 20 negative samples in pork, chicken, and urine samples. The negative and positive coincidence rates in pork samples are 100%, and the false positive rate in chicken samples is less than 7%, and the positive coincidence rate is 100%. %, the negative coincidence rate was above 90%. The false positive rate in the urine sample is ...

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Abstract

The invention provides an immune colloidal gold test paper card for testing diazepam. The test paper card comprises a reaction membrane, a sample pad, a gold marker pad, a water adsorption pad and a back sheet, the reaction membrane has a test area coated with a diazepam hapten-carrier protein conjugate and a quality control area coated with goat anti mouse IgG, and a conjugate releasing pad is coated with a diazepam monoclonal antibody-colloidal gold marker. The invention further provides a method utilizing the test paper card for testing diazepam. The method comprises the steps that firstly,a sample is pretreated, then the pretreated sample is tested by the test paper card, and finally, the test result is analyzed. The test paper card can be used for testing the amount of residual diazepam medicine in animal-derived food and urine samples, and is simple in operation, high in sensitivity, high in testing speed, low in cost, and capable of achieving on-site supervision and meeting therequirements for screening a large number of samples.

Description

technical field [0001] The invention relates to rapid detection of immunochemistry, in particular to a colloidal gold test strip for detecting residues of diazepam, capable of rapidly detecting residues of diazepam. Background technique [0002] Diazepam, as a sedative, is widely used in most livestock and poultry to ensure that the livestock and poultry will not have a stress response during long-distance transportation. Sexual deficiency symptoms and mental dependence may even lead to drug dependence. Sudden withdrawal of the drug will cause drug withdrawal reaction, depression, violent mental fluctuations, insomnia and other symptoms. It is strictly prohibited to be used in animal husbandry in my country and the European Union. In order to protect human health and safety, it is very important to detect the residues of diazepam drugs in animal food. [0003] For the detection of diazepam residues, the country formulates corresponding testing standards, such as "GB 29688-...

Claims

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Application Information

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IPC IPC(8): G01N33/577
Inventor 万宇平何方洋冯才伟扶胜袁光宇罗维超谢体波程茹李平张凯
Owner 贵州勤邦食品安全科学技术有限公司
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