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Preparation method of pancreatin

A pancreatin and pancreas technology, applied in pancreatin and other directions, can solve the problems of uncertain inactivation effect, high cost and inadvisability of ultra-high pressure equipment, and achieve the effect of avoiding pancreatin activity and complete inactivation effect.

Active Publication Date: 2021-03-02
江苏麦德森制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this method is that the inactivation effect of high pressure treatment on some viruses is uncertain, and the ultra-high pressure equipment is expensive and the production cost is high, which is basically not advisable in production practice

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0025] The preparation of trypsin comprises the following steps carried out in sequence:

[0026] (1) Fresh and frozen raw materials: 25kg of pancreas and duodenum;

[0027] (2) Raw material pretreatment: thaw the freshly frozen raw material, soak in 50L of 75% ethanol containing 0.5% peracetic acid, soak at 5°C for 6 hours, drain and wash;

[0028] (3) Refining: Grinding the pretreated pancreas raw materials into pancreas pulp;

[0029] (4) Activation and extraction: Add 50L of ethanol aqueous solution with a pH of 3~4 and a concentration of 25% to the pancreatic juice, add 250g of trisodium phosphate by volume, stir for 2 hours, add 250g of calcium acetate, and The temperature is 10-15°C and the reaction is extracted with stirring for 6 hours;

[0030] (5) Precipitation: The extract is refrigerated and centrifuged to remove impurities, and the pancreas is collected to extract the centrifugate. Add ethanol to the centrifuge to a concentration of 75% ethanol, precipitate try...

Embodiment approach 2

[0035] (1) Fresh and frozen raw materials: 25kg of pancreas and duodenum;

[0036] (2) Raw material pretreatment: thaw the freshly frozen raw material, soak in 50L of 75% ethanol containing 1% peracetic acid, soak for 6 hours at 5°C, drain and wash;

[0037] (3) Refining: Grinding the pretreated pancreas raw materials into pancreas pulp;

[0038] (4) Activation and extraction: Add 50L of ethanol aqueous solution with a pH of 3~4 and a concentration of 25% to the pancreatic juice, add 500g of trisodium phosphate by volume, stir for 2 hours, add 500g of calcium acetate, Stirring reaction extraction at 10-15°C for 6 hours;

[0039] (5) Precipitation: The extract was refrigerated and centrifuged, the pancreas was collected to extract the centrifugate, and ethanol was added to the centrifuge to a concentration of 75% ethanol, the trypsin was precipitated, and the precipitate was collected by centrifugation;

[0040] (6) Degreasing and drying: Add 5 times the amount of acetone to ...

Embodiment approach 3

[0044] (1) Fresh and frozen raw materials: 25kg of pancreas and duodenum;

[0045] (2) Raw material pretreatment: thaw the freshly frozen raw material, soak in 50L of 75% ethanol containing 1.5% peracetic acid, soak for 6 hours at 5°C, drain and wash;

[0046] (3) Refining: pancreas raw materials are ground into pancreas pulp after pretreatment;

[0047] (4) Activation and extraction: Add 50L of ethanol aqueous solution with a pH of 3~4 and a concentration of 25% to the pancreatic pulp, add 750g of trisodium phosphate by volume, stir for 2 hours, add 750g of calcium acetate, and heat at 10 ~15°C, stirring reaction extraction for 6 hours;

[0048] (5) Precipitation: The extract was refrigerated and centrifuged, the pancreas was collected to extract the centrifugate, and ethanol was added to the centrifuge to a concentration of 75% ethanol, the trypsin was precipitated, and the precipitate was collected by centrifugation;

[0049] (6) Degreasing and drying: Add 5 times the amo...

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Abstract

The invention relates to the technical field of biology and discloses a pancreatin preparation method. The pancreatin preparation method comprises the steps: unfreezing pancreas, utilizing 75% ethyl alcohol containing 0.1 to 2% of virus inactivating agent to soak the pancreas for 1 to 6 hours to inactivate virus, drying, washing, grinding the pretreated pancreas into pulp, adding trisodium phosphate and a low-concentration organic solvent and adding calcium salt to perform activation and extraction; separating and filtering to remove impurities of endotoxin and the like; precipitating; dryingand smashing to obtain a product. According to the pancreatin preparation method disclosed by the invention, a pancreas pretreatment technology can make 75% ethyl alcohol containing peracetic acid quickly permeate animal tissues; meanwhile, phosphate and a calcium salt activating agent are added in an extracting process to form calcium phsopahte, the endotoxin can be removed, and the purpose of inactivating virus can be more effectively achieved; the technology is concese and easy to operate, enzyme activity and yield of prepared pancreatin cannot be affected, peracetic acid residues can meetthe requirement, and safety of pancreatin medicine is greatly enhanced.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a preparation method of trypsin. Background technique [0002] Pancreatin is a digestive drug recorded in the pharmacopoeias of various countries. It is a mixture of various enzymes extracted from animal pancreas. The main components are trypsin, pancreatic lipase and pancreatic amylase. Pancreatin is mainly used for indigestion, loss of appetite, and digestive disorders caused by liver and pancreatic diseases. It is also suitable for congenital pancreatic insufficiency, abdominal surgery, and pancreatic insufficiency caused by traumatic pancreatectomy. In addition, pancreatin contains a variety of active substances, which can be used as basic raw materials to extract a variety of biochemical drugs, such as pancreatic kininogen, elastase, asparaginase, chymotrypsin, carboxypeptidase, etc. In addition, pancreatin is also It is widely used in cell culture to produce therapeutic biolog...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/94
Inventor 刘榜惠
Owner 江苏麦德森制药有限公司
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