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Method for preparing alkali xylanase preparation through spray drying process

A xylanase, alkaline technology, applied in the field of microbial fermentation technology and enzyme preparations, can solve the problems of late start, scarce independent products, low enzyme activity of products, etc., to protect the environment, reduce the environmental protection load of enterprises, and produce enzyme activity. improved effect

Inactive Publication Date: 2019-05-28
问清江
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Alkaline xylanase produced by microbial fermentation started relatively late in my country, most of which are in the basic research stage, domestic independent products are scarce, and the enzyme activity of the products is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] The steps of the method for preparing alkaline xylanase preparation by spray drying are:

[0012] Step 1: Fermentation:

[0013] Put the liquid medium A into the fermenter and adjust the pH to 8.0. After routine sterilization, insert the activated and cultured Bacillus pumilus strains according to the volume ratio of 5% aseptically. min, ventilation rate 28L / min, tank pressure 0.05MPa, fermentation cycle 24h; stop fermentation when the enzyme activity measured by 3,5-dinitrosalicylic acid method reaches 2500IU / ml;

[0014] Step 2: Preparation of enzyme preparation:

[0015] Use a high-efficiency plate-and-frame filter to remove the bacteria and insoluble impurities in the fermentation broth under a pressure of 0.1 MPa to obtain a brown clear liquid as an enzyme liquid; add dextrin in a mass ratio of 10%, stir evenly, ℃, spray drying under the condition of air outlet temperature 90 ℃, and obtain the finished product of powdery solid alkaline xylanase preparation, the p...

Embodiment 2

[0021] The steps of the method for preparing alkaline xylanase preparation by spray drying are:

[0022] Step 1: Fermentation:

[0023] Put the liquid medium A into the fermenter and adjust the pH to 7.8. After routine sterilization, insert the activated and cultured Bacillus pumilus strains according to the volume ratio of 3.5% aseptically. min, ventilation rate 35L / min, tank pressure 0.04MPa, fermentation cycle 30h; stop fermentation when the enzyme activity measured by 3,5-dinitrosalicylic acid method reaches 2500IU / ml;

[0024] Step 2: Preparation of enzyme preparation:

[0025] Use a high-efficiency plate and frame filter to remove the bacteria and insoluble impurities in the fermentation broth under a pressure of 0.08MPa to obtain a brown clear liquid as an enzyme liquid; add dextrin in a mass ratio of 8%, stir evenly, ℃, spray drying under the condition of air outlet temperature 80 ℃, obtain the finished product of powdery solid alkaline xylanase preparation, the prod...

Embodiment 3

[0031] The steps of the method for preparing alkaline xylanase preparation by spray drying are:

[0032] Step 1: Fermentation:

[0033] Put the liquid medium A into the fermenter and adjust the pH to 7.5. After routine sterilization, insert the activated and cultured Bacillus pumilus strains according to the volume ratio of 2.0% aseptically. min, ventilation rate 25L / min, tank pressure 0.03MPa, fermentation period 36h; stop fermentation when the enzyme activity measured by 3,5-dinitrosalicylic acid method reaches 2500IU / ml;

[0034] Step 2: Preparation of enzyme preparation:

[0035] Use a high-efficiency plate-and-frame filter to remove the bacteria and insoluble impurities in the fermentation broth under a pressure of 0.05 MPa to obtain a brown clear liquid as an enzyme liquid; add dextrin at a mass ratio of 12%, stir evenly, ℃, spray drying under the condition of air outlet temperature 90 ℃ to obtain the finished powdery solid alkaline xylanase preparation, the product en...

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Abstract

The invention relates to the technical field of microbial fermentation and enzymic preparation and specifically relates to a method for preparing an alkali xylanase preparation by combining bran fermentation with spray drying. Bran fermentation is adopted; the alkali xylanase is generated by fermenting bran with bacillus pumilus; enzyme-generating activity of fermentation liquor is 2700IU / ml and is greatly improved; the fermentation liquor is prepared into the alkali xylanase preparation through spray drying, so that the salting out of ammonium sulfate is avoided and the emission of effluent is greatly reduced; the enzyme-generating activity during the preparation process of alkali xylanase preparation is increased by more than four times, the production efficiency is greatly increased andthe technical industrialization is benefited. The method provided by the invention comprises the processing steps of 1) fermenting and 2) preparing the enzyme preparation.

Description

technical field [0001] The invention relates to the technical fields of microbial fermentation technology and enzyme preparation, in particular to a method for preparing alkaline xylanase preparation by spray drying. Background technique [0002] Xylanase (Xylanase) is an enzyme that specifically degrades xylan, which belongs to the class of hydrolytic enzymes, including a variety of endonucleases and exonucleases. The alkaline xylanase produced by Bacillus pumilus is stable and has the strongest effect under alkaline conditions, and can hydrolyze xylan into xylooligosaccharides or xylose. Therefore, it can be used in papermaking, ramie processing and other cellulose manufacturing industries. Alkaline xylanase can selectively partially hydrolyze hemicellulose (mainly xylan) without damaging the fiber strength and reduce chemical substances in subsequent processes. reduce the pollution of harmful chemicals to the environment. The preparation of alkaline xylanase preparation...

Claims

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Application Information

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IPC IPC(8): C12N9/24C12N9/96C12R1/07
Inventor 问清江慕娟孙晓宇
Owner 问清江
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