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Fluorescence immunoassay test paper for quantitative joint detection of multiple indexes of ovarian reserve function

A fluorescent immunity and ovarian reserve technology, applied in the field of fluorescent immunoassay test strips, can solve the problems of inability to easily and quickly detect multiple indicators, low degree of automation, radioactive contamination, etc., to achieve reliable detection results, reduce detection costs, Accurate effect

Active Publication Date: 2019-05-24
石家庄洹众生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The RIA method has radioactive pollution, while the IRMA method is time-consuming and laborious, and the degree of automation is not high
However, none of the above methods can simply and quickly detect multiple indicators related to ovarian reserve function.

Method used

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  • Fluorescence immunoassay test paper for quantitative joint detection of multiple indexes of ovarian reserve function
  • Fluorescence immunoassay test paper for quantitative joint detection of multiple indexes of ovarian reserve function

Examples

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preparation example Construction

[0032] In the present invention, the sample pad is preferably a whole blood filtration sample pad. The material of the sample pad is preferably a glass cellulose membrane; the preparation method of the sample pad preferably includes the following steps: after soaking the glass cellulose membrane in the blocking solution, dry; the soaking time is preferably 3-7min, more preferably 5min; the blocking solution preferably includes the following components: 0.05wt% Tris, 1wt% tritonX-100, 0.5wt% PEG6000, 0.4wt% bovine serum albumin and 0.02wt% mouse antibody human red blood cells.

[0033]In the present invention, after the binding pad is lapped on the sample pad, the binding pad is sprayed with a fluorescent microsphere-labeled monoclonal antibody mixture; the monoclonal antibody mixture includes the first follicle-stimulating hormone monoclonal antibody, The first luteinizing hormone monoclonal antibody, the first anti-Müllerian hormone monoclonal antibody and the first serum in...

Embodiment 1

[0046] The structure of fluorescent immunoassay paper combined with quantitative detection of multiple indicators of ovarian reserve function

[0047] A fluorescent immunological test strip for combined quantitative detection of multiple indicators of ovarian reserve function. The test strip comprises a sample pad, a binding pad, a nitrocellulose membrane and absorbent paper sequentially connected to a PVC bottom plate. structured as figure 1 Shown; wherein the PVC bottom plate of the test strip is sequentially stuck with nitrocellulose membrane (4), absorbent paper (5), binding pad (3), and a sample pad (2) is laid on the upper layer of the binding pad; Among them, 1mm wide FSH detection line (6-1), LH detection line (6-2), AMH detection line (6-3), INHB detection line (6-4) are drawn in parallel on the nitrocellulose membrane (4). ) and quality control line (7), the distance between each detection line and the quality control line is 8mm; after assembly, cut according to th...

Embodiment 2

[0062] (1) Drawing of fitting standard curve

[0063] Linear dilution of standard antigen: use negative bovine serum to linearly dilute FSH and LH standard antigen: 150mIU / mL, 100mIU / mL, 75mIU / mL, 50mIU / mL, 25mIU / mL, 10mIU / mL, 5mIU / mL, 0mIU / mL; AMH standard antigen was linearly diluted with negative bovine serum: 10ng / mL, 5ng / mL, 2.5ng / mL, 1ng / mL, 0.5ng / mL, 0.25ng / mL, 0.1ng / mL, 0ng / mL mL; linearly dilute INHB standard antigen with negative bovine serum: 2000pg / mL, 1000pg / mL, 500pg / mL, 250pg / mL, 100pg / mL, 50pg / mL, 25pg / mL, 0pg / mL. Add the standard sample to the sample hole of the test paper, use the same batch of test paper and reagents, repeat the measurement for each concentration more than 3 times, use the fluorescence immunoassay analyzer to obtain the fluorescence intensity data of the detection line and the quality control line, analyze the data, and The concentration of the standard is the abscissa, and the fluorescence intensity ratio (T / C) of the detection line and t...

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Abstract

The invention belongs to the technical field of medical testing and provides fluorescence immunoassay test paper for quantitative joint testing of multiple indexes of an ovarian reserve function. Thetest paper comprises a sample pad, a conjugate pad, a nitrocellulose membrane and water absorbing paper in sequential connection with a PVC base plate. Fluorescent microsphere marked monoclonal antibody mixed liquid is sprayed to the conjugate pad and comprises a first follicle-stimulating hormone monoclonal antibody, a first luteinizing hormone monoclonal antibody, a first anti-mullerian hormonemonoclonal antibody and a first serum inhibin B monoclonal antibody. The nitrocellulose membrane is provided with four test lines and a quality control line in sequential parallel arrangement, and thefour test lines are coated with a second follicle-stimulating hormone monoclonal antibody, a second luteinizing hormone monoclonal antibody, a second anti-mullerian hormone monoclonal antibody and asecond serum inhibin B monoclonal antibody respectively. The fluorescence immunoassay test paper has advantages of simplicity in operation, low errors, reliability in test result and high accuracy.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a fluorescent immunological test strip for combined quantitative detection of multiple indicators of ovarian reserve function. Background technique [0002] Ovarian reserve function refers to the ability of follicles in the ovarian cortex to grow, develop, and form fertilizable oocytes, that is, the quantity and quality of eggs remaining in the ovary. With age or other factors, the number and quality of eggs decrease, that is, the decline of ovarian reserve function. There are many factors affecting ovarian reserve function, including age, genetic factors, autoimmune factors, surgery on the ovary and surrounding tissues, and social and psychological factors. The decline of ovarian reserve function is a gradual process. Early detection and diagnosis, timely treatment and intervention can effectively improve women's ovarian function, pregnancy ability and pregnancy...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/533
Inventor 李晓春张少丹
Owner 石家庄洹众生物科技有限公司
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