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Lc/ms/ms analysis for meat speciation in raw and processed meat product

A meat and sample technology, applied in the direction of analysis of materials, material separation, biological testing, etc., can solve the problem of not detecting multiple protein markers, etc.

Active Publication Date: 2019-05-21
DH TECH DEVMENT PTE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, ELISA methods only detect a subset of proteins and do not detect multiple protein markers

Method used

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  • Lc/ms/ms analysis for meat speciation in raw and processed meat product
  • Lc/ms/ms analysis for meat speciation in raw and processed meat product
  • Lc/ms/ms analysis for meat speciation in raw and processed meat product

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example

[0094] Applicant's teachings may be more fully understood with reference to the following examples and resulting data. Other embodiments of the present teachings will be apparent to those skilled in the art from consideration of the specification and practice of the present teachings disclosed herein. These examples are intended to be considered exemplary only. Figure 17 An exemplary flow diagram for preparing a meat sample according to some embodiments of the present teachings is shown.

[0095] Prepare the solution

[0096] Extraction Buffer - Tris(2-amino-2-(hydroxymethyl)-1,3-propanediol, 0.61 g), urea (36.04 g) and thiourea (7.61 g) were dissolved in HPLC grade water and made up to 100 mL volume to prepare extraction buffer containing 50 mM Tris, 6 M urea, and 1 M thiourea.

[0097] Ammonium Bicarbonate Buffer - Ammonium Bicarbonate (158 g) was made up to a volume of 20 mL with HPLC water to make a 100 mM solution.

[0098] Reducing agent - tris-(2-carboxyethyl)-ph...

example 2

[0151] Meat from cooked burgers obtained from fast food restaurants was purchased and analyzed according to this teaching. Figure 15 Depicted using SEQ ID NO:5 (upper left two), SEQ ID NO:6 (upper right two), SEQ ID NO:7 (lower left two) and SEQ ID NO: Extracted ion chromatograms of beef peptides of 8 (bottom right two).

[0152] Figure 16 Extracted ion chromatograms depicting pork, lamb, chicken, and duck, indicating the absence of contamination from other meats in the burger sample compared to the background.

[0153] Alternative Methods of Sample Preparation

[0154] Other methods of meat sample preparation prior to analysis are now described.

[0155] Homogenization and protein extraction of meat samples using a bead homogenizer

[0156] After weighing, freezing and grinding the meat samples according to the previously described method, 1 g of each ground meat product was placed in a 20 ml centrifuge tube and 5 stainless steel balls (3 mm) were added. 5 mL of ex...

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Abstract

Apparatus, methods and kits for detecting the contamination of a meat sample with another type of meat using parent-daughter ion transition monitoring that identifies peptides specific to a particulartype of meat. The meat types detected can include pork, beef, lamb, chicken, duck and / or horse and one or more combinations thereof.

Description

[0001] related application [0002] This application claims priority to US Provisional Application No. 62 / 384,925, filed September 8, 2016, the entire contents of which are incorporated herein by reference. technical field [0003] This case involved the analysis and identification of contamination in meat samples. Background technique [0004] The Food Standards Agency (FSA) (an independent government department in the UK) announced in January 2013 that horse and / or pig deoxyribonucleic acid (DNA) molecules had been identified in beef products sold by several supermarket chains. Follow-up testing across and beyond Europe has shown widespread occurrence of such contamination. The presence of other meat species in presumed pure samples of specific meat products has been of general concern. Contamination may be the result of unwanted amounts of one or more of pork, beef, lamb, chicken, duck, and / or horse meat being present in the meat product. [0005] However, most existi...

Claims

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Application Information

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IPC IPC(8): G01N30/72G01N33/12G01N33/68G01N30/88G01N30/06
CPCG01N33/12G01N30/7233G01N2030/8813G01N33/6848G01N30/88H01J49/004
Inventor 郭立海刘华芬斯蒂芬·洛克牟思覃培斌熊蕾
Owner DH TECH DEVMENT PTE
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