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A decomposed solid fermentation agent and its application in agricultural waste

A technology for agricultural waste and solid fermentation, which is applied in microorganism-based methods, bacteria, fungi, etc., can solve the problems of polluted soil air, surface groundwater, soil heavy metals exceeding the standard, blackening and odor, etc. Strong enzyme activity, new effect of strain combination

Active Publication Date: 2020-11-13
保定市科绿丰生化科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In addition, the rapid development of the breeding industry is accompanied by a rapid increase in the production of livestock and poultry manure. Livestock and poultry manure contains a large amount of organic matter and is easy to breed pathogenic microorganisms. If it is not properly treated, it will cause serious harm to the environment, such as causing diseases and parasites. The spread and spread of insect eggs, the breeding of mosquito ropes, the deterioration of water quality and even black and smelly, unrestrained excessive direct discharge will cause heavy metals in the soil to seriously exceed the standard, and pollute the soil, air, surface and groundwater, etc.

Method used

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  • A decomposed solid fermentation agent and its application in agricultural waste
  • A decomposed solid fermentation agent and its application in agricultural waste
  • A decomposed solid fermentation agent and its application in agricultural waste

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Experimental program
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Effect test

Embodiment 1

[0031] Embodiment 1: the preparation of decomposed solid fermentation bacterial agent

[0032] The present invention provides a kind of preparation of decomposed solid fermentation bacterial agent, and specific preparation method comprises the following steps:

[0033] (1) The slant strains stored in the environment of 0-4°C were activated, Saccharomyces cerevisiae, Bacillus methylotrophicus, Geobacillus thermodenitrificans , Bacillus simplex and Lactobacillus parabuchneri. The strains were cultured: Saccharomyces cerevisiae and Lactobacillus parabuchneri were cultured using FC2 medium; the formula of the FC2 medium was: 2% sucrose; 0.5% yeast extract; 0.3% peptone; 0.2% Disodium hydrogen phosphate; the inoculum size is 1%-2%, 180-200rpm, cultured for 24-36h. FC2 medium, Bacillus methylotrophicus and Geobacillus thermodenitrificans adopt optimized YM1 medium, the formula of the medium is: 0.5% corn steep liquor; 0.2% disodium hydrogen phosphate ; 1% sucrose; the inoculum si...

Embodiment 2

[0038] Embodiment two: the preparation of decomposed solid fermentation bacterial agent

[0039] The slant strains stored in the environment of 0-4°C were activated, and the five strains were cultured in test tubes and shake flasks respectively to obtain the first-grade seed liquid of each strain; Microscopic direct counting method and ultraviolet spectrophotometry were used to detect the absorbance value of the fermentation liquid and the number of flora to judge the growth status of the flora, and the effective number of viable bacteria was detected to be 1×10 9 cfu / ml or so, the culture was stopped; after the fermentation of each single strain, Saccharomyces cerevisiae, Bacillus methylotrophicus, Geobacillus thermodenitrificans, Bacillus simplex ( Bacillus simplex) and Lactobacillus parabuchneri (Lactobacillus parabuchneri) were mixed at a weight ratio of 1:2:2:3:3 to obtain a decomposed liquid composite fermentation bacterial agent; the obtained composite bacterial agent l...

Embodiment 3

[0040] Embodiment three: the preparation of decomposed solid fermentation bacterial agent

[0041] Based on Example 2, each bacterial strain is activated and cultivated to obtain the primary seed liquid of each bacterial strain; the seed liquid is continued to be expanded and cultivated in a fermenter, and it is detected that the number of effective viable bacteria is 1 × 10 9 Stop the culture when the cfu / ml is about; after the fermentation of each single strain, Saccharomyces cerevisiae, Bacillus methylotrophicus, Geobacillus thermodenitrificans, Bacillus simplex simplex) and Lactobacillus parabuchneri (Lactobacillus parabuchneri) in a ratio of 1:2:2:1:1 by weight to obtain a decomposed liquid compound fermentation agent; Mix with light calcium carbonate and stir evenly, dry until the water content is below 15%; add potassium sorbate protective agent with a mass concentration of 1-1.5‰, stir evenly, and then obtain a decomposed solid fermentation bacterial agent.

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Abstract

The invention discloses a decomposed solid fermentation fungicide using agricultural organic wastes such as cow dung and straws (walnut pruning branches and cotton stalks) as raw materials, includingfive bacteria of saccharomyces cerevisiae, bacillus methylotrophicus, geobacillus thermodenitrificans, bacillus simplex and lactobacillus parabuchneri. The invention also discloses a method for preparing the decomposed solid fermentation fungicide. The active fungicide utilizes the synergistic effect between the five bacteria in the composting materials to rapidly form a dominant flora and fully exerts the deodorization and decomposing process promoting effects. At the same time, the problem that the effective utilization rate of the agricultural organic wastes is not high, the natural fermentation composting time is long, and the product quality is poor is solved. A fertilizer subjected to decomposed fermentation can improve the soil fertility of the farmland, regulate the pH of the soil,and has good ecological and economic benefits.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and further relates to the technical field of a decomposed solid fermentation bacterial agent and its application in the utilization of agricultural waste. Background technique [0002] In recent years, with the rapid development of the forestry and fruit industry, the planting area of ​​walnuts, red dates, apricots, apples, grapes, etc. has continued to expand, and the random stacking of a large number of pruned agricultural solid wastes has occupied a large amount of arable land and easily caused the spread of crop diseases. Incineration will cause air pollution problems. The annual output of all kinds of agricultural waste in my country exceeds 700 million tons, which is a valuable renewable resource. The use of agricultural solid waste decomposing and returning technology can improve soil organic matter content, improve soil physical properties, fertilize soil fertility, and i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/18C05F15/00C05F17/20C05F17/50C12R1/865C12R1/07C12R1/225C12R1/01
CPCY02W30/40
Inventor 谢玉清冯蕾陈竞代金平杨新平张慧涛古丽·艾合买提王志方买尔哈巴·艾合买提秦新政王小武
Owner 保定市科绿丰生化科技有限公司
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