Immobilized enzyme pickering emulsion reaction system and application thereof

Abstract

The invention discloses an immobilized enzyme pickering emulsion reaction system and an application thereof. The immobilized enzyme pickering emulsion reaction system comprises an immobilized enzyme using mesoporous nanometer materials as a carrier, and an oil phase and a water phase for forming emulsion, wherein the emulsion particle diameter of the system is 10-80[mu]m, reaction raw materials are used as the oil phase, a buffer solution is used as the water phase, and the immobilized enzyme using the mesoporous nanometer materials as a carrier is used as a catalyst and an emulsifying agent.In the pickering emulsion enzymic method reaction system, the reaction raw materials are directly used as the oil phase, and the immobilized enzyme of the mesoporous nanometer materials is used as a reaction catalyst and besides, is the emulsifying agent of pickering emulsion. Compared with a conventional emulsion added with an organic reagent or an emulsifying agent, the catalysis activity and the stability are notably improved, products are easy to separate and purify, and repeated use and scaled enlargement are facilitated, the application range is broad, and the immobilized enzyme pickering emulsion reaction system is green and environmental-friendly.

Description

technical field [0001] The invention belongs to the technical field of biocatalysis, and relates to the construction and application of an immobilized enzyme Pickering emulsion reaction system. Background technique [0002] Enzymes are green and efficient biocatalysts. However, in some high-viscosity or heterogeneous reaction systems, the free enzyme has poor stability, low activity, and is difficult to recycle and reuse, which greatly limits its application in industry. Some researchers improve the viscosity of the reaction system by adding organic reagents, but there will be problems of product separation and organic solvent residue. Pickering emulsion refers to the emulsion formed by using solid particles as emulsifier, which can not only reduce the reaction energy, speed up the reaction process, but also facilitate the separation and recovery of emulsifier and product. At present, immobilized enzymes and Pickering emulsions have been widely used in food, medicine, ener...

AI Technical Summary

Problems solved by technology

[0003] At present, a large number of researched Pickering emulsion enzymatic reaction systems use enzyme solution or diluted enzyme solution as the water phase, and additional organic solvents are added as the oil phase. It is attempted to use different types of materials to prepare Pickering emulsions, but the addition of organic solvents It will bring cumbersome separation and purification steps, and it is easy to produce residues, which is not suitable for the preparation of food-grade products

Patent CN107955808 A discloses a preparation method based on double-sided particle-stabilized Pickering emulsion and its immobilized enzyme application, using diluted free enzyme solution and buffer as the water phase, not only reducing the enzyme concentration but also reducing the enzyme and substrate Contact area, catalytic activity decreased

Patent CN 107973919 A ​​discloses a preparation method based on dopamine-stabilized Pickering emulsion and its immobilized enzyme application, which improves the stability of the emulsion and enzyme by adding dopamine, but its cost is high, the operation is complicated, and the product is separated Purification is difficult and the scope of application is limited

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