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Method for Screening Potentially Important Genes in Population Genome Based on mk-test

A genome and gene technology, applied in the field of MK-test-based population genome screening of potentially important genes, can solve problems such as heavy workload and inability to determine the function of a single gene, and achieve the effect of convenient operation and reasonable design.

Active Publication Date: 2022-06-28
中国科学院昆明植物研究所海盐工程技术中心
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Problems solved by technology

However, these methods have a large workload for the screening of potentially important genes in population genomes, and cannot determine the function of individual genes in plants.

Method used

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  • Method for Screening Potentially Important Genes in Population Genome Based on mk-test
  • Method for Screening Potentially Important Genes in Population Genome Based on mk-test
  • Method for Screening Potentially Important Genes in Population Genome Based on mk-test

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Experimental program
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Embodiment 1

[0063] MK-test-based methods for screening potentially important genes in population genomes, including:

[0064] 1) Build a gene interaction network: collect gene interaction data from BioGRID, IntAct, DIP, MINT and STRING databases, and separate the interaction data of target plants to establish a corresponding database;

[0065] 2) Use a processing module to obtain and / or input gene parameters as needed, wherein the processing module includes at least two gene chips, a computer connected to the gene chip; the gene parameters include: species, population genes, number of nucleotides with differences between genes, and total number of genes. number of nucleotides;

[0066] 3) After the gene parameters are calculated by the canonical formula, the inter-species and / or intra-species variation rates are output, and the potentially important genes of the population genome are screened out;

[0067] 4) Functional analysis of potentially important genes;

[0068] The above calcula...

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Abstract

The invention provides a method for screening potentially important genes of a population genome based on MK-test, which belongs to the technical field of gene screening, including: 1) constructing a gene interaction network; 2) using a processing module to obtain and / or input gene parameters as required; 3) After the gene parameters are calculated by a standard formula, the inter-species and / or intra-species variation rates are output, and the potentially important genes of the population genome are screened out; 4) the functional analysis of the potentially important genes is carried out; wherein the processing module includes at least two A gene chip, a computer connected to a gene chip. The present invention can simultaneously analyze the differences between species or within species in large batches, and can effectively analyze the trend of the ratio of non-expressed segments and / or the ratio of expressed segments with changes in genes after calculation, providing a basis for screening potentially important genes in population genomes an efficient path.

Description

technical field [0001] The present invention relates to the technical field of gene screening, in particular to a method for screening potentially important genes in a population genome based on MK-test. Background technique [0002] Molecular population genetics is a pillar subject of contemporary evolutionary biology research, as well as a basic theoretical subject of genetic breeding and genetic association mapping and linkage analysis. Molecular population genetics is developed on the basis of classical population genetics. It uses macromolecules, mainly the variation patterns of DNA sequences, to study the genetic structure of the population and the relationship between the factors that cause population genetic changes and the genetic structure of the population. Scientists can quantitatively infer the evolution of a population accurately, which not only overcomes the limitation of classical population genetics that usually only studies short-term changes in the genetic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G16B20/50
Inventor 章成君金桂花石勇胡彦婷李玲
Owner 中国科学院昆明植物研究所海盐工程技术中心
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