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Method for deriving aldehyde pyrimidine, method for detecting 5-aldehyde cytosine as well as application of aldehyde pyrimidine derivative

A technology of aldehyde cytosine and aldehyde pyrimidine, which can be used in measurement devices, instruments, analytical materials, etc., can solve problems such as harsh conditions and long time consumption, and achieve the effects of mild conditions, prolonged retention time, and long time consuming.

Active Publication Date: 2019-04-26
SICHUAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0011] The purpose of the present invention is to provide a method for derivatizing aldehydepyrimidine, a method for detecting 5-formylcytosine and the application of aldehydepyrimidine derivatives, so as to solve the time-consuming and conditional problems existing in the existing 5fC detection and identification process. harsh question

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  • Method for deriving aldehyde pyrimidine, method for detecting 5-aldehyde cytosine as well as application of aldehyde pyrimidine derivative
  • Method for deriving aldehyde pyrimidine, method for detecting 5-aldehyde cytosine as well as application of aldehyde pyrimidine derivative
  • Method for deriving aldehyde pyrimidine, method for detecting 5-aldehyde cytosine as well as application of aldehyde pyrimidine derivative

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Embodiment 1

[0049] The method for derivatizing aldehyde pyrimidines in this embodiment includes:

[0050] Mix aldehyde pyrimidine and Wittig reagent in ethanol at a molar ratio of 1:1, react at 15°C for 48 hours, and then irradiate with ultraviolet light with a wavelength of 200nm for 24 hours to prepare aldehyde pyrimidine derivatives; wherein, The aldepyrimidine is 5-formylcytosine or 5-formyluracil.

Embodiment 2

[0052] The method for derivatizing aldehyde pyrimidines in this embodiment includes:

[0053] Mix aldepyrimidine and Wittig reagent in N,N-dimethylformamide at a molar ratio of 1:500, react at 80°C for 5 minutes, and then irradiate with ultraviolet light with a wavelength of 400nm for 1 minute to obtain Formaldehyde pyrimidine derivatives; wherein, formaldehyde pyrimidine is 5-formyl cytosine or 5-formyl uracil.

Embodiment 3

[0055] The method for derivatizing aldehyde pyrimidines in this embodiment includes:

[0056] Mix aldehyde pyrimidine and Wittig reagent in ethylene glycol dimethyl ether at a molar ratio of 1:20, react at 50°C for 6 hours, and then irradiate aldehyde pyrimidine with a wavelength of 253 nm for 12 hours to prepare aldehyde pyrimidine Derivatives; wherein, the formyl pyrimidine is 5-formyl cytosine or 5-formyl uracil.

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Abstract

The invention discloses a method for deriving aldehyde pyrimidine, a method for detecting 5-aldehyde cytosine as well as application of an aldehyde pyrimidine derivative. The aldehyde pyrimidine derivative is prepared by mixing aldehyde pyrimidine and a Wittig reagent in an organic solvent and performing radiation by ultraviolet light, wherein the aldehyde pyrimidine is 5-aldehyde cytosine or 5-aldehyde uracil. The reaction condition is mild, efficient and rapid, application of the Wittig reagent in the field of epigenetics is effectively enlarged, and new though is provided for design of a chemical marker and a reaction type 5fC fluorescent probe of the aldehyde pyrimidine.

Description

technical field [0001] The invention relates to the technical field of nucleic acid chemistry, in particular to a method for derivatizing aldehyde pyrimidine, a method for detecting 5-formyl cytosine and the application of aldehyde pyrimidine derivatives. Background technique [0002] In mammalian cell or tissue DNA, especially CpG islands, there are different degrees of methylation modification, which is closely related to embryonic development, tumor, mental, blood system and other major diseases. DNA methylation refers to the process in which DNA methyltransferase (DNMT) uses adenomethionine as a methyl donor to add a methyl group to the 5-carbon of cytosine to convert it into 5-methylcytosine (5mC). process, and this process is dynamically reversible, that is, DNA demethylation. DNA demethylation can be divided into two different forms, active and passive. The former is due to the inhibition of the DNA methylation maintenance mechanism, such as the inactivation of DNMT...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D239/47C07D239/54C07D471/04G01N21/64
CPCC07D239/47C07D239/54C07D471/04G01N21/6428G01N2021/6439
Inventor 李坤周倩余孝其
Owner SICHUAN UNIV
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