Combined vaccine for bacterial septicemia and red skin disease of grass carps, and preparation method of combined vaccine

Abstract

The invention discloses a combined vaccine for bacterial septicemia and red skin disease of grass carps, and a preparation method of the combined vaccine. The safe and efficient two-tier white oil inactivated vaccine against aeromonas hydrophila septicaemia and pseudomonas aeruginosa red skin disease is developed by using beta-propiolactone as an inactivator, and can effectively control the occurrence of the bacterial septicemia and red skin disease of the grass carps. After the grass carps are immunized with the vaccine, the vaccine can stimulate the expression of IgM, IL-1beta, IFN, Mx, C3 and TLR3 immune-related genes in fish spleen and head kidney tissues. The vaccine can produce up to 80-90% relative immune protection rate for the grass carps; the relative protection rate can still bemaintained at 50% after immunization is performed for one year. The combined vaccine is simple to operate, is safe and effective, can prevent two diseases after being used for immunizing at a time, and not only reduces the use of antibiotics and chemical drugs, but also reduces the pollution and destruction of the external environments such as breeding environment and ecological environment, thusguaranteeing the food safety and water ecological security of the grass carps.

Description

technical field [0001] The invention belongs to the field of fish farming, and in particular relates to a combined vaccine of grass carp bacterial sepsis and red skin disease and a preparation method thereof. Background technique [0002] Grass carp is one of the main breeding species in Guangdong Province, with an annual output of more than 4 million tons, but the annual loss due to disease is as high as more than 2 billion yuan. Grass carp septicemia and red skin disease are extremely serious bacterial diseases that harm grass carp, with a wide range of prevalence and high mortality. Grass carp bacterial sepsis is caused by the infection of Aeromonas hydrophila. It is a high-incidence and high-risk disease of grass carp in recent years. The onset season is long and the epidemic range is wide, and the disease occurs to varying degrees in the main breeding areas of grass carp across the country. Grass carp red skin disease has plagued grass carp farmers in my country for a...

AI Technical Summary

Problems solved by technology

[0003] For the prevention and control of grass carp bacterial diseases, tissue plasma vaccines or single bacterin immunization methods are mostly used, but none of them can achieve satisfactory results

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