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Method for detecting expression level of FASN gene in bovine muscle

A technology of gene expression and bovine muscle, applied in the field of genetic engineering, to achieve the effect of easy operation and simple method

Inactive Publication Date: 2019-04-19
INNER MONGOLIA AGRICULTURAL UNIVERSITY
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

At present, there are few reports about this gene in bovine muscle

Method used

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  • Method for detecting expression level of FASN gene in bovine muscle
  • Method for detecting expression level of FASN gene in bovine muscle

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Embodiment Construction

[0021] In order to enable those skilled in the art to better understand the solution of the present invention, the technical solution of the present invention will be further described below in conjunction with specific examples.

[0022] A method for detecting bovine muscle FASN gene expression, the specific steps are as follows:

[0023] 1) Design the specific primer pair F-F and F-R of the FASN gene sequence and the primer pair G-F and G-R of the internal reference gene:

[0024] Referring to the FASN gene sequence (Genbank Accession No.NM-001012669) recorded in Genbank, and the internal reference gene GAPDH (Genbank Accession No.0NM-001034034), primers were designed using Primer Premier 3.0 software, and the specificity of the primers was tested through the Blast function in NCBI .

[0025] F-F: 5'-GTGAAGCCCATTGAGGACAT-3', see SEQ NO.1 in the sequence listing.

[0026] F-R: 5'-AGCTGCACGTGTTCTGTCAC-3', see SEQ NO.2 in the sequence listing.

[0027] The sequence of the pr...

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Abstract

The invention discloses a method for detecting the expression level of an FASN gene in bovine muscle. According to the method, the expression level of the FASN gene in the bovine muscle is accuratelyand quickly analyzed through the design of an FASN gene-specific primer pair, the qPCR amplification of an internal reference primer GAPDH and relative quantitative analysis of a qPCR result. The qPCRdetection method for detecting the expression level of the FASN gene can be used for accurately detecting the expression level of the FASN genes in the muscle of cattle of different breeds. The method can be used for detecting the expression level of the FASN genes in the muscle of the cattle of different breeds, provides a reference for further research on the functions of the bovine FASN gene,and is simple and easy to operate.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to a method for detecting the expression of bovine muscle FASN gene. Background technique [0002] Fatty acid synthase (FASN) is a multi-enzyme complex system composed of 6 enzymes. FASN is the rate-limiting enzyme for fat synthesis, and its activity directly controls the synthesis of body fat. In mammals, only the FASN in dimer form has the activity of catalyzing fatty acid synthesis, and the monomer form of FASN does not have the activity of catalyzing fatty acid synthesis, but some functional domains of FASN still have catalytic activity in the monomer. The substrate is transferred to the acyl carrier protein (Acyl carrier protein, ACP) functional domain in advance and the activity of the functional domain interacting with ACP is reduced. [0003] Using the methods of in situ hybridization and somatic cell hybridization, the bovine FASN gene was located on chromosome 19. The f...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/6851
CPCC12Q1/6851C12Q1/6888C12Q2600/158C12Q2531/113C12Q2545/101C12Q2563/107
Inventor 敖日格乐斯木吉德王纯洁
Owner INNER MONGOLIA AGRICULTURAL UNIVERSITY
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