Honeysuckle content detecting method

A detection method, the technology of honeysuckle, applied in the field of traditional Chinese medicine research, can solve the problems of high cost, cumbersome preparation process of chromatographic method, and high requirements for operators

Pending Publication Date: 2019-04-16
TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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Problems solved by technology

However, the result of manual identification depends on the identification experience of the appraiser, is highly subjective, and cannot be tested in large quantities; the chromatographic method can give the accurate content information of the tested substance, but the solid sample needs to be extracted from traditional Chinese medicine first, and the chromatographic method The preparation process in the early stage of the method is cumbersome, requires additional reagent assistance, has high requirements for operators, high cost, and long test time, which cannot meet the needs of real-time detection
Not suitable for on-site inspection of market purchases and mass inspection of incoming materials from pharmaceutical factories

Method used

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Examples

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Embodiment 1

[0036] figure 1 It is a flowchart of the method for detecting the content of honeysuckle in Example 1, including sample collection, spectrum collection, selection of characteristic bands, model building and model application.

[0037] 1. Collection of samples: The experimental samples were 1 batch of honeysuckle from Henan and 3 batches of mountain silver flower from Hunan, which were crushed and passed through a No. 4 sieve. 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, mixed, take each 5 grams each of the mixtures, a total of 51 sample sets were obtained. Put the powder of the sample collection into the sample holders respectively, and tap lightly until it is tightly packed.

[0038] 2. Near-infrared spectrum collection of sample set: the near-infrared spectrum collection instrument is Fourier near-infrared spectrometer produced by Thermo Fisher Company, the software is Result software, and the spectral scanning range is 4000-10000cm-1 , a tota...

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Abstract

The invention provides a honeysuckle content detecting method, which belongs to the research field of traditional Chinese medicine. The invention provides a honeysuckle content detecting method basedon a near infrared spectroscopy technology. Compared with an evaluation method by an evaluator and a chromatographic method, the determination time can be greatly shortened, dependency on the evaluation experience of the evaluator does not happen, a large amount of reagent is not needed, no complex pre-stage sample preparation is needed, a large amount of manpower and material resources are saved,and thus, a new method is provided for standardizing the market of honeysuckle medicinal materials and ensuring the quality reliability of honeysuckle medicinal plants.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine research, in particular to a method for detecting the content of honeysuckle. Background technique [0002] The name of honeysuckle comes from "Compendium of Materia Medica", also known as "Honeysuckle", and the dried flower buds of honeysuckle are used as medicine in clinical medicine, which has the effect of clearing away heat and detoxifying. Lonicera japonica is a plant of the Lonicera family, which is widely produced in southern China. Lonicera argentina, Lonicera red glandularis, Lonicera chinensis or Lonicera japonica are all classified as Lonicera japonica. Honeysuckle and mountain silver flower are similar in shape, and it is difficult to distinguish them with the naked eye. However, the two have different medicinal value. "Chinese Pharmacopoeia" (2015 edition) uses the content of chlorogenic acid and luteolin as the quality evaluation standard of honeysuckle; uses ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/359
CPCG01N21/359G01N2021/3595
Inventor 赵静崔彭帝刘唤李正王海霞
Owner TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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