Microfluidic chip with preset magnetic beads, manufacturing method and microfluidic detection equipment

Abstract

A microfluidic chip with preset magnetic beads, including a core body and a thin film. A microfluidic channel is opened on the core body, and the thin film covers the microfluidic channel. The inlet end of the microfluidic channel is connected with a liquid inlet channel. The outlet end of the flow channel is provided with a valve, the part of the micro-channel close to the outlet end is connected with a waste liquid discharge channel, the middle part of the micro-channel expands to form a magnetic bead reaction tank, and the bottom of the magnetic bead reaction tank is provided with a water-soluble inclusion and The preset magnetic bead coating formed by mixing magnetic beads, the core body is fixed with a preset soft magnet, and the preset soft magnet is located on the back side of the magnetic bead reaction tank. The invention provides a microfluidic chip with pre-set magnetic beads, a manufacturing method and a microfluidic detection device, which can precisely control the amount of magnetic beads captured and the trajectory of the magnetic beads, and improve the adsorption / elution effect of the magnetic beads.

Description

technical field [0001] The invention relates to the field of microfluidic technology, in particular to a microfluidic chip with preset magnetic beads, a manufacturing method and a microfluidic detection device. Background technique [0002] Magnetic polymer microspheres, especially microspheres with superparamagnetic properties, will be instantly magnetized and demagnetized under the action of an external magnetic field. As a new type of solid-phase carrier reagent, Fe3O4 magnetic beads can be chemically modified and modified on the surface, and specific chemical groups can also specifically bind to many types of biomolecules. Therefore, they can be used in various Separation, purification and analysis of biomolecules. The magnetic bead method makes the solid-liquid separation of various biomolecules more precise and convenient, so it is widely used in the fields of biological or medical analysis and detection such as nucleic acid extraction, immune reaction, and cell separ...

AI Technical Summary

Problems solved by technology

There are some studies on the problem of mixing magnetic beads and various reaction solutions in the prior art, but all of them have obvious defects

Traditional magnetic bead capture systems are mostly single-sided magnet static systems, such as a microfluidic chip detection method based on magnetic bead technology disclosed in patent CN107942050A, which uses an adjustable strong magnet to achieve sample washing and elution. It is difficult to achieve uniform mixing between magnetic beads after beading, which will easily lead to sample residue and affect subsequent operations; patent CN206838098U discloses a centrifugal immunomagnetic bead sorting microfluidic chip and device, which combines the rotating shaft of the centrifuge with the chip At the same time, the mixing and separation between samples is achieved by using clockwise and counterclockwise conversion operations. This scheme has relatively high restrictions, which is not conducive to the multi-functional integration of the chip, and the high speed is not conducive to the subsequent reaction; also as the application number 201410368536.9 The Chinese invention patent discloses a microfluidic chip magnetic bead chaotic mixing method and device, which changes the magnetic poles of the electromagnetic coil to make the magnetic beads mix and move, but the electromagnetic coil will generate heat accumulation during operation, which is easy to damage

Moreover, this kind of active electromagnet has a large volume, and magnets need to be arranged in multiple positions, which takes up space. The movement of magnetic beads needs to be realized by adjusting the magnetic force in different directions, which is also time-consuming.

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