Ecological purification method of oyster culture
A technology of ecological purification and oysters, applied in chemical instruments and methods, fish farming, climate change adaptation, etc., can solve problems such as death, disease of cultured animals, failure to complete, etc.
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Embodiment 1
[0031] The ecological purification method of oyster culture comprises, using the culture that disperses p-hydroxybenzaldehyde and sodium o-nitrophenolate to cultivate probiotics based on extreme environments; fixing the probiotics on a carrier to make a microecological preparation; The oysters are cultured in the environment with the probiotics until harvested. When probiotics are cultivated, the stimulation of external extreme environments can be involved, which can increase the adaptability of probiotics to external environments such as low temperature, high temperature, hyperosmosis, peroxidation, etc., improve their anti-stress ability, and prolong their service life. This increases the purification efficiency of the probiotics on the aquaculture water body, strengthens the purification effect, and reduces the cost of ecological purification.
[0032] Probiotics include photosynthetic bacteria and Bacillus subtilis. Probiotics can decompose and purify nitrite, ammonia nit...
Embodiment 2
[0045] The ecological purification method of oyster culture specifically comprises the following steps:
[0046] 1) The culture medium was prepared according to the formula of MS culture medium, using sterilized seawater instead of distilled water as the substrate, and adding 1% and 0.7% p-hydroxybenzaldehyde and sodium o-nitrophenolate as bioaccelerators respectively, and the probiotics After activation, insert 10% of the inoculum into the above-prepared culture medium, shake and culture at 35°C for 15 days, then place the culture system in one of the extreme environments for 12 hours, and then transfer it to a normal culture environment. Shake culture at 35°C for 15 days, then place the culture system in another extreme environment for 12 hours, then transfer to the normal culture environment, and repeat the alternate culture for 65 days to complete. The above extreme environments specifically include the following: temperature 7°C, Cl - Concentration 1.5%, H 2 o 2 Concen...
Embodiment 3
[0051] The ecological purification method of oyster culture specifically comprises the following steps:
[0052] 1) The culture medium was prepared according to the formula of MS culture medium, using sterilized seawater instead of distilled water as the substrate, and adding 0.75% and 0.55% p-hydroxybenzaldehyde and sodium o-nitrophenolate as bioaccelerators respectively, and the probiotics After activation, insert 8.5% of the inoculum into the above-prepared culture medium, shake and culture at 33°C for 12 days, then place the culture system in one of the extreme environments for 8 hours, and then transfer it to the normal culture environment. Shake culture at 33°C for 12 days, then place the culture system in another extreme environment for 8 hours, then transfer it to a normal culture environment, and repeat the alternate culture for 60 days to complete. The above extreme environments specifically include the following: temperature 7°C, Cl - Concentration 1%, H 2 o 2 Co...
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