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Ecological purification method of oyster culture

A technology of ecological purification and oysters, applied in chemical instruments and methods, fish farming, climate change adaptation, etc., can solve problems such as death, disease of cultured animals, failure to complete, etc.

Active Publication Date: 2021-05-11
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There is another major source of water pollution in aquaculture: excessive bait is put in during high-density aquaculture, and the physiological activities of organisms are vigorous in an environment with sufficient bait, which promotes the increase of ammonia nitrogen in the water body; input and uneaten feed, excrement of cultured animals Suspension for a long time reduces the transparency of the water body, inhibits the growth of algae and the absorption of ammonia nitrogen in the water body, exacerbates the problem of low dissolved oxygen in the water body, and gradually deteriorates the water body. If the accumulation reaches a certain level, it will cause the disease or even death of the cultured animals, causing huge losses.
[0005] For the purification method of aquaculture water, the existing technology is to add chemical oxygen release agents, beneficial microorganisms, disinfectants, disease-resistant agents, etc. to the water body. Although some progress has been made, there are also deficiencies, mainly: the addition of After releasing the active ingredients, the substance ends its mission and can only have a short-term limited effect on the water body. It needs to be added continuously, and sometimes has side effects on the cultured animals; the added beneficial microorganisms cannot survive for a long time at the bottom of the water body, and Changes in environmental conditions will affect their growth and decline, sometimes causing secondary pollution; general disinfectants often kill the original beneficial microorganisms, and the original beneficial ecology is destroyed
[0006] The prior art, such as the Chinese invention patent with the authorized announcement number CN1309666C, discloses an ecological method for controlling nitrite in fish and shrimp ponds. It uses bacillus bacteria to carry out ecological purification, but the bacteria used is relatively simple and cannot be completed or Can only be purified weakly, not very practical in actual production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The ecological purification method of oyster culture comprises, using the culture that disperses p-hydroxybenzaldehyde and sodium o-nitrophenolate to cultivate probiotics based on extreme environments; fixing the probiotics on a carrier to make a microecological preparation; The oysters are cultured in the environment with the probiotics until harvested. When probiotics are cultivated, the stimulation of external extreme environments can be involved, which can increase the adaptability of probiotics to external environments such as low temperature, high temperature, hyperosmosis, peroxidation, etc., improve their anti-stress ability, and prolong their service life. This increases the purification efficiency of the probiotics on the aquaculture water body, strengthens the purification effect, and reduces the cost of ecological purification.

[0032] Probiotics include photosynthetic bacteria and Bacillus subtilis. Probiotics can decompose and purify nitrite, ammonia nit...

Embodiment 2

[0045] The ecological purification method of oyster culture specifically comprises the following steps:

[0046] 1) The culture medium was prepared according to the formula of MS culture medium, using sterilized seawater instead of distilled water as the substrate, and adding 1% and 0.7% p-hydroxybenzaldehyde and sodium o-nitrophenolate as bioaccelerators respectively, and the probiotics After activation, insert 10% of the inoculum into the above-prepared culture medium, shake and culture at 35°C for 15 days, then place the culture system in one of the extreme environments for 12 hours, and then transfer it to a normal culture environment. Shake culture at 35°C for 15 days, then place the culture system in another extreme environment for 12 hours, then transfer to the normal culture environment, and repeat the alternate culture for 65 days to complete. The above extreme environments specifically include the following: temperature 7°C, Cl - Concentration 1.5%, H 2 o 2 Concen...

Embodiment 3

[0051] The ecological purification method of oyster culture specifically comprises the following steps:

[0052] 1) The culture medium was prepared according to the formula of MS culture medium, using sterilized seawater instead of distilled water as the substrate, and adding 0.75% and 0.55% p-hydroxybenzaldehyde and sodium o-nitrophenolate as bioaccelerators respectively, and the probiotics After activation, insert 8.5% of the inoculum into the above-prepared culture medium, shake and culture at 33°C for 12 days, then place the culture system in one of the extreme environments for 8 hours, and then transfer it to the normal culture environment. Shake culture at 33°C for 12 days, then place the culture system in another extreme environment for 8 hours, then transfer it to a normal culture environment, and repeat the alternate culture for 60 days to complete. The above extreme environments specifically include the following: temperature 7°C, Cl - Concentration 1%, H 2 o 2 Co...

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PUM

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Abstract

The invention provides an ecological purification method for oyster cultivation, which belongs to the field of aquaculture. Specifically, the method is to cultivate probiotics based on extreme environments by using cultures dispersed with p-hydroxybenzaldehyde and sodium o-nitrophenolate, and then combine the above-mentioned probiotics and The microalgae are immobilized on the carbonized straw carrier to make a microecological preparation, and then the oysters are cultured in the environment where the above microecological preparation is placed until harvesting. The ecological purification method for oyster cultivation provided by the present invention can reduce the heavy metal content in the oyster body, improve the quality of the oyster, significantly increase the fattening, increase dissolved oxygen, and have a good purification effect, can significantly reduce the cost of cultivation and purification, and realize waste utilization, ecological balance and health The purpose of breeding: the immobilized microecological preparation used in the method has strong anti-stress ability, large immobilization capacity, high immobilization degree, high stability, long service life and storage time, and wide adaptability.

Description

technical field [0001] The invention belongs to the field of aquaculture, and in particular relates to an ecological purification method for oyster cultivation. Background technique [0002] In recent decades, my country's marine shellfish farming has made great progress. However, with the rapid economic development of my country's coastal areas, the environmental pollution of coastal waters has become increasingly serious, and the problem of shellfish pollution has become more and more serious. This problem not only restricts the healthy development of China's shellfish aquaculture industry, but also causes huge economic losses to my country's shellfish aquaculture industry, and also brings a series of public food safety problems. [0003] Generally speaking, the sources of polluted shellfish can be divided into three categories: heavy metals, pesticides, petroleum hydrocarbons, etc. from industrial pollutants; microbial pollutants from domestic sewage; and biological toxin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/54C02F3/34C02F103/20
CPCA01K61/54C02F3/34C02F2103/20Y02A40/81
Inventor 景斐胡利腾张建设
Owner ZHEJIANG OCEAN UNIV
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