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WP52 strain for degrading unsymmetrical dimethylhydrazine and applications thereof

A technology of unsymmetrical dimethylhydrazine and strains, which is applied in the field of microorganisms and achieves the effects of high efficiency, simple process and wide suitable range.

Active Publication Date: 2019-04-12
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few research reports on the biodegradation of UDM at home and abroad, and there are even fewer microbial strains that can degrade UDM, and they mainly focus on the production of UDM at low and medium concentrations (≤50mg / L). Biodegradable

Method used

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  • WP52 strain for degrading unsymmetrical dimethylhydrazine and applications thereof
  • WP52 strain for degrading unsymmetrical dimethylhydrazine and applications thereof
  • WP52 strain for degrading unsymmetrical dimethylhydrazine and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Screening and Identification of Bacterial Strain WP52

[0031] 1. Strain screening

[0032] (1) Strain isolation and purification

[0033] Collect soil samples around the launch site, add the soil samples at a ratio of 5% to the inorganic salt liquid medium with a concentration of unsymmetrical dimethylhydrazine of 40-300mg / L, and place them at a temperature of 28-37°C and a speed of 150 After culturing at -200r / min for 2-7d, streak and separate on beef extract peptone, inorganic salt and potato solid medium, culture at a temperature of 28-37°C for 2-7d, and continue to isolate the strain on the above plate Continue to draw the plate until the purified strain is obtained.

[0034] At the same time, get the soil sample, prepare soil sample according to the dilution coating method: normal saline is a suspension of 1:9, and continue to dilute with normal saline to obtain 10 -1 -10 -3 Suspension, shake for 10-30 minutes, take 100ul and spread on inorganic sal...

Embodiment 2

[0068] Growth characteristics of embodiment 2 bacterial strain WP52

[0069] 1. Strain WP52 grows in different media

[0070] The unsymmetrical dimethylhydrazine degrading strain WP52 of the present invention can grow in various mediums, such as beef extract peptone medium, potato medium, inorganic salt medium and unsymmetrical dimethylhydrazine as the only inorganic salt medium of nitrogen source Can grow in medium and easy to cultivate.

[0071] Inorganic salt medium: K 2 HPO 4 1.000-3.000g / L, KH 2 PO 4 1.000-3.000g / L, NaCl 5.000-8.000g / L, MgSO 4 0.200-0.800g / L, CaCl 2 0.010-0.050g / L, FeSO 4 0.005-0.010g / L, H 3 BO 3 0.100-0.200mg / L, ZnSO 4 0.100-0.200mg / L, unsymmetrical dimethylhydrazine 40-300mg / L, glucose 0.500-5.000g / L.

[0072] 2. Growth curve of strain WP52

[0073] Inoculate the strain WP52 into a 100mL Erlenmeyer flask containing 50mL beef extract peptone liquid medium according to the inoculum amount of 3-5%, and cultivate it for 24 hours under the ...

Embodiment 3

[0081] Degradation characteristics of embodiment 3 bacterial strain WP52 to unsymmetrical dimethylhydrazine

[0082] 1. Degradation of strains to different concentrations of unsymmetrical dimethylhydrazine

[0083] The concentration of unsymmetrical dimethylhydrazine was set to three different gradient concentrations of high, medium and low, and the degradation experiment was carried out. Among them, the high concentration of unsymmetrical dimethylhydrazine is ≥100mg / L, the medium concentration is 40mg / L, and the low concentration is ≤40mg / L.

[0084] Activation of strain WP52: the method is the same as that of 4 above.

[0085] Inoculation and degradation: Inoculate the activated bacterial liquid with 2-10% inoculum amount in unsymmetrical dimethylhydrazine as the only nitrogen source, the concentrations are 40mg / L, 60mg / L, 80mg / L, 100mg / L, 120mg / L respectively Inorganic salt liquid medium, and then placed in a shaker with a temperature of 28-37°C and a rotation speed of 16...

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Abstract

The invention relates to pseudomonas extremaustralis WP52 capable of degrading unsymmetrical dimethylhydrazine, and belongs to the technical field of microorganisms. The strain was preserved on June 11, 2018 in the China General Microbiological Culture Collection Center, and the preservation number is: CGMCC No.15921. The strain WP52 of the invention has wide optimal growth condition range, is easy to culture, has degradation effect on low, medium and high-concentrations of unsymmetrical dimethylhydrazine, has high efficiency and low cost, can be applied to bioremediation of water and soil contaminated by unsymmetrical dimethylhydrazine, and achieves the purpose of completely degrading unsymmetrical dimethylhydrazine.

Description

technical field [0001] The invention relates to an unsymmetrical dimethylhydrazine degrading bacterial strain and application thereof, belonging to the technical field of microbes. Background technique [0002] Unsymmetrical dimethylhydrazine (UDMH), the molecular formula is C 2 h 8 N 2 , it is a high-energy fuel widely used in the launch of spacecraft such as missiles, satellites and spacecraft. The inadvertent discharge of unsymmetrical dimethylhydrazine during production, transportation or storage, as well as the large amount of waste water generated during the launch process will pollute the environment. my country has strict restrictions on the allowable concentration of unsymmetrical dimethylhydrazine in the environment. [0003] The treatment methods of unsymmetrical dimethylhydrazine in the environment mainly include physical, chemical and biological methods. Physical and chemical methods have problems such as incomplete treatment, high cost or secondary polluti...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C02F3/34A62D3/02C12R1/38C02F101/38A62D101/06A62D101/26
CPCA62D3/02C12N1/20C02F3/34B09C1/10C02F2101/38A62D2101/26A62D2101/06C12R2001/38C12N1/205
Inventor 李艳菊吴月曾庆轩王建营王力杨正礼
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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