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Controllable preparation method of liposome vesicle based on microfluidic device

A technology of liposome vesicles and microfluidic devices, applied in liposome delivery, chemical instruments and methods, medical preparations of non-active ingredients, etc., can solve problems such as liposome inhomogeneity, and achieve repeatable production Good performance, improved utilization rate, and simple post-treatment process

Inactive Publication Date: 2019-04-12
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In order to solve the uneven problem of liposome preparation in the traditional method, the purpose of the present invention is to overcome the deficiencies in the prior art, and to provide a controllable preparation method of liposome vesicles based on a microfluidic device. The method prepares macroscopic and large-volume liposome vesicles with uniform properties, and provides a fast, efficient, flexible and controllable method for preparing liposomes

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  • Controllable preparation method of liposome vesicle based on microfluidic device
  • Controllable preparation method of liposome vesicle based on microfluidic device
  • Controllable preparation method of liposome vesicle based on microfluidic device

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Embodiment 1

[0023] In this embodiment, see Figure 1 ~ Figure 4 , A controllable preparation method of liposome vesicles based on a microfluidic device, which adopts the principle of self-assembly preparation induced by interface reaction, and the steps are as follows:

[0024] a. Using a microfluidic device, the microfluidic device is a "Y"-shaped microfluidic chip with a serpentine mixing channel, containing two injection ports and one outlet, forming a main channel connected by two branch channels. "Y" microchannel structure, two branch channels form two precursor solution injection ports, the end of the main channel forms a product outlet, the total length of the serpentine mixing channel is 4cm; the main microchannel of the "Y" microfluidic chip The channel is a serpentine mixed channel connected in sequence with four "S"-shaped structures; the four "S"-shaped structures of the main microchannel of the "Y"-shaped microfluidic chip in this embodiment are four "bow"-shaped structures in s...

Embodiment 2

[0043] This embodiment is basically the same as the first embodiment, and the special features are:

[0044] In this embodiment, a method for the controllable preparation of liposome vesicles based on a microfluidic device adopts the self-assembly preparation principle initiated by an interface reaction, and the steps are as follows:

[0045] a. This step is the same as the first embodiment;

[0046] b. This step is the same as the first embodiment;

[0047] c. Use a constant pressure injection pump to inject the components of the precursor solution in step b into the branch microchannels of the "Y"-shaped microfluidic chip in step a through different injection ports, and set The total flow rate of the precursor solution, adjust the flow rate ratio of the hydroxyethylpiperazine ethanesulfonic acid buffer and the phospholipid molecular alcohol solution, and collect the product at the outlet of the main microchannel of the "Y"-shaped microfluidic chip. The product is the prepared produc...

Embodiment 3

[0052] This embodiment is basically the same as the previous embodiment, and the special features are:

[0053] In this embodiment, a method for the controllable preparation of liposome vesicles based on a microfluidic device adopts the self-assembly preparation principle initiated by an interface reaction, and the steps are as follows:

[0054] a. This step is the same as the first embodiment;

[0055] b. This step is the same as the first embodiment;

[0056] c. Use a constant pressure injection pump to inject the components of the precursor solution in step b into the branch microchannels of the "Y"-shaped microfluidic chip in step a through different injection ports, and set The total flow rate of the precursor solution, adjust the flow rate ratio of the hydroxyethylpiperazine ethanesulfonic acid buffer and the phospholipid molecular alcohol solution, and collect the product at the outlet of the main microchannel of the "Y"-shaped microfluidic chip. The product is the prepared pro...

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Abstract

The invention provides a preparation method of a liposome vesicle based on a microfluidic device. The preparation method comprises the steps of: preparing a microfluidic chip by a soft lithography method; preparing a precursor solution for preparing the liposome vesicle; and using a constant-pressure injection pump to inject the precursor solution into a ''Y''-shaped chip through different injection ports, setting a total flow rate, adjusting the flow ratio of a buffer solution to a phospholipid molecular alcohol solution, and collecting a product at an outlet, wherein the product is the prepared liposome vesicle. The microfluidic chip selected by the invention is a ''Y''-shaped microfluidic chip with a serpentine mixing channel of a square structure, and the chip is prepared by the soft lithography method. By using the chip, based on the principle of self-assembly on an interface, by adjusting the total flow rate and the flow rate ratio of the aqueous buffer solution to the phospholipid molecule ethanol solution, regulation of the size of the liposome vesicle and control on the dimensional uniformity are achieved, and an efficient low-cost method is provided for preparation of artificial cells with uniform properties as well as drug carriers.

Description

Technical field [0001] The invention relates to a method for preparing artificially synthesized biomass materials, in particular to a method for preparing artificially synthesized nano lipid vesicles, which are applied to the technical fields of gene therapy and artificial cells. Background technique [0002] Phospholipids are amphiphilic molecules, and when dispersed in an aqueous environment, they can self-assemble into vesicles. In nature, large lipid vesicles act as membranes in biological cells to protect intracellular components from the extracellular environment. The function of nanometer-sized small lipid vesicles is to transport biological molecules within the cell. The separation of lipid membranes gives biological systems complexity and functionality. Artificially synthesized nano-lipid vesicles can be customized for various applications with different surface characteristics, for example, nano-lipid vesicles are used for targeted drug delivery. After encapsulating r...

Claims

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Application Information

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IPC IPC(8): B01L3/00A61K9/127A61K47/24
CPCA61K9/127A61K47/24B01L3/502707B01L3/502769B01L2200/0605B01L2200/14
Inventor 龚秀清王晓红刘进丰
Owner SHANGHAI UNIV
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