Method for industrialized co-production of phycocyanin, spirulina polysaccharide and protein feed using fresh spirulina

Abstract

The invention discloses a method for industrial co-production of phycocyanin, spirulina polysaccharide and protein feed from fresh spirulina. The method comprises the following steps: (1), taking cleaned fresh spirulina sludge into an ultrasonic extraction tank; (2), uniformly stirring the mixed solution, and performing ultrasonic wall breaking extraction; (3), extracting the mixed solution for solid-liquid separation; (4), performing separation on an algae residue / grinding bead mixture by a filter screen; (5), cleaning and recycling grinding beads; (6), performing solid-liquid separation on the algae residue mixed solution again by a fine filter; (7), passing the combined phycocyanin extract through cross-flow membrane filtration equipment; (8), passing the supernatant in the previous step through 30 KDa ultrafiltration membrane equipment; (9), spray drying an ultrafiltration concentrated solution. The method has the following advantages that no chemical additive is used, physical wall breaking is conducted, pure water extraction is conducted in the whole process, the extraction cost is reduced, no chemical sewage is discharged, the method is environmentally friendly, and the application requirement of the natural and healthy product of phycocyanin is met.

Description

technical field [0001] The invention relates to a method for industrially co-producing phycocyanin, spirulina polysaccharide and protein feed by using fresh spirulina, which belongs to the technical field of biological extraction. Background technique [0002] Phycocyanin is a dark blue powder isolated from spirulina. Mainly found in cyanobacteria, red algae and cryptophyta. Phycocyanin is usually also divided into C-phycocyanin and R-phycocyanin, the former mainly exists in cyanobacteria, the latter mainly exists in red algae, both of which are found in cryptophycobacteria. Its function is to absorb light (orange yellow) energy and transmit light energy. It is not only a kind of protein, but also an excellent natural food coloring, and it is also a good health food. [0003] At present, all extraction methods use freeze-thaw plus high-pressure homogenization or freeze-thaw plus buffer immersion. The freeze-thaw method consumes a lot of energy, and the buffer immersion me...

AI Technical Summary

Problems solved by technology

[0003] At present, all extraction methods use freeze-thaw plus high-pressure homogenization or freeze-thaw plus buffer immersion. The freeze-thaw method consumes a lot of energy, and the buffer immersion method requires a large amount of chemical additives. It takes 3 days to extract, which is not conducive to continuous and rapid production

[0004] At present, almost all extraction methods use dried spirulina powder as raw material for extraction, and fresh spirulina algae mud needs to be dried in advance. First, the energy consumption, manpower, time and equipment required for drying are increased. In addition, during the drying process The high temperature will reduce the content of phycocyanin in spirulina

[0005] The general ultrasonic extraction method has poor wall breaking effect and low phycocyanin dissolution rate, so it cannot be applied to industrial production

[0006] At present, all industrial extraction methods can only extract phycocyanin, which cannot realize the industrial co-production of phycocyanin and spirulina polysaccharides, and cannot make full use of the nutritional value of spirulina raw materials, and the economic benefits are not high

There are many reports on methods for simultaneously extracting phycocyanin, chlorophyll, or spirulina polysaccharides and other nutrients, but they are still in the laboratory test stage, the operation is complicated, and a large amount of organic solvents are used for step-by-step extraction, which cannot be applied to large-scale industrial production.

[0007] All current industrial extraction methods cannot effectively and quickly eliminate bacteria during the production process, and the quality of phycocyanin is poor