Method for determination of ecotoxicity of soil contaminated by residues of organophosphorus and pyrethroid insecticides

A technology for pyrethroids and contaminated soil, which is applied to the determination of the ecological toxicity of soil contaminated by pyrethroid insecticides and in the field of organic phosphorus. Effect of short time consumption and shortened measurement time

Inactive Publication Date: 2019-04-05
SOUTHWEST FORESTRY UNIVERSITY
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Problems solved by technology

[0006] Aiming at the problems that the existing methods for detecting the ecotoxicity of soil polluted by pesticide residues and pesticides take a long time and have a single detection value, the present invention proposes a method for measuring the ecotoxicity of soil polluted by organic phosphorus and pyrethroid pesticide residues

Method used

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  • Method for determination of ecotoxicity of soil contaminated by residues of organophosphorus and pyrethroid insecticides
  • Method for determination of ecotoxicity of soil contaminated by residues of organophosphorus and pyrethroid insecticides
  • Method for determination of ecotoxicity of soil contaminated by residues of organophosphorus and pyrethroid insecticides

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Effect test

Embodiment 1

[0027] Example 1: The application of earthworm HSP27 in the monitoring of ecotoxicity of organophosphate chlorpyrifos soil pollution is mainly divided into three steps, namely: exposure of earthworms; extraction and isolation of earthworm HSP27; detection of HSP27 expression. The obtained results are shown in Table 1, and the abnormal value of HSP27 expression was 20 mg / kg.

[0028] Table 1 The expression of HSP27 in earthworms treated with different doses of chlorpyrifos pesticide and the control group

[0029]

[0030] a: There is a significant difference between the treatment group and the control group at the level of α=0.05

[0031]The growth toxicity of chlorpyrifos soil pollution to earthworms was determined by using the existing earthworm growth toxicity test method. The method refers to Khalil et al. (1996). The test results are shown in Table 2. The results in Table 2 show that the pesticide concentration of chlorpyrifos in the soil reaches above 20.0mg / kg, and t...

Embodiment 2

[0036] Example 2: The application of earthworm HSP27 in the monitoring of ecotoxicity of pyrethroid insecticide cypermethrin soil pollution is mainly divided into three steps, namely: exposure of earthworms; extraction and separation of earthworm HSP27; detection of HSP27 expression. The obtained results are shown in Table 1, and the abnormal value of HSP27 expression was 40 mg / kg.

[0037] Table 3 Expression of HSP27 in earthworms treated with different doses of cypermethrin pesticide and control group

[0038]

[0039] a: There is a significant difference between the treatment group and the control group at the level of α-0.05

[0040] The existing earthworm growth toxicity determination method was used to determine the growth toxicity of cypermethrin soil pollution to earthworms. The method was referred to Khalil et al. (1996). The test results are shown in Table 4. The results in Table 4 show that the concentration of cypermethrin pesticide in the soil reaches above 40...

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Abstract

The invention belongs to the field of environmental protection, and particularly relates to a method for determination of the ecotoxicity of soil contaminated by residues of organophosphorus and pyrethroid insecticides. The method is characterized in that eisenia foetida is selected, and after being domesticated, the eisenia foetida is subjected to an exposure test in the soil to be tested; through the steps of protein extraction, SDS-PAGE protein isolation and Western Blotting detection purity analysis, if a test group and a control group are significantly different and p is less than 0.05, it is indicated that pesticide residues in the soil to be tested are toxic to soil biological growth; and if no significant difference exists, the soil to be tested is not toxic to growth. The method is suitable for early diagnosis and early warning of contaminated soil, greatly shortens the determination time of ecotoxicity, and effectively improves the diagnostic efficiency of soil ecotoxicity.

Description

technical field [0001] The invention belongs to the field of environmental protection, and specifically relates to a method for measuring the ecotoxicity of organic phosphorus and pyrethroid pesticide residual polluted soil. Background technique [0002] Organophosphorus and pyrethroid insecticides are the most commonly used pesticide types in my country. Most of these two types of pesticides are non-polar insecticides, which have strong biological toxicity and are easily absorbed and fixed by the soil. Extensive use of organophosphorus and pyrethroid insecticides can easily lead to soil pesticide residue pollution, directly threatening the health and safety of human food and the sustainable development of soil ecological functions. [0003] Molecular markers are a kind of biomarkers, which can be used as early signals of the harmful effects of pollutants, and can predict and forecast the effects at the individual level and above the individual level under pollution conditio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/24G01N33/68
CPCG01N33/24G01N33/68G01N2333/43504
Inventor 周世萍杨发忠刘永梅刘守庆梁坤雷然朱国磊郭佳葳袁智勇吕天雯夏红云
Owner SOUTHWEST FORESTRY UNIVERSITY
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