Nano sensor and preparation method and application thereof

Abstract

The invention provides a nano sensor capable of simultaneously and real-time monitoring glucose and oxygen, and a preparation method and application thereof. The nano sensor is a nano sensor having acore-shell structure, and has a glucose probe, an oxygen probe and a reference probe. The nano sensor can simultaneously realize ratiometric detection of glucose and oxygen without interfering with each other; and the nano sensor dispersion can stably exist for at least half a year without a precipitating agent. At the same time, the sensor has a relatively small size of up to 100 nm, has good cell permeability and low cytotoxicity, can be used for intracellular sensing and can monitor intracellular glucose and oxygen in real time.

Description

technical field [0001] The invention belongs to the technical field of sensors, and relates to a nanometer sensor and its preparation method and application, in particular to a nanometer sensor capable of monitoring glucose and oxygen simultaneously and in real time, and its preparation method and application. Background technique [0002] Cellular metabolic processes are central to cellular function, response to stimuli, and survival. Many complex diseases, including cancer, are directly associated with altered regulation of metabolic pathways at different levels; altered cellular metabolism is considered one of the hallmarks of cancer. Despite remarkable advances in our understanding of metabolic changes associated with disease states, the dynamics of metabolic processes, especially at the single-cell level, are lacking. Therefore, methods to monitor intracellular metabolism have become an urgent scientific problem. Glucose and oxygen concentrations are two important par...

AI Technical Summary

Problems solved by technology

Winkler titration, also known as chemical method or iodometric method, is used as a standard method for measuring oxygen concentration within a certain range in the world. In my country, it is used as the oxygen concentration detection method specified in the national standard. Its advantage is high measurement accuracy and repeatability. However, the iodometric method is only suitable for clean water samples. When the water contains other ions such as iron ions, free chlorine ions and nitrite, it will interfere with the test results; more importantly, the method takes a long time to titrate , the use of a large number of chemical reagents leads to cumbersome procedures, and will continue to consume oxygen, and the requirements of on-site testing cannot be met. These defects greatly limit the use of iodometric method

The second is electrochemical analysis, that is, the Clark electrode method; the electrochemical method is simple to operate, can achieve continuous measurement, and can meet the requirements of on-site sample testing. Due to the presence of a selectively permeable polytetrafluoroethylene film, the requirements for samples are relatively low. However, due to its special structure and detection principle, it needs to be preheated for a few minutes before use to keep the voltage stable; the temperature and the dirt on the film will directly affect the test effect of the Clark battery, so it is necessary to clean the dirt on the electrode and film in time. Frequent, it is best to replace the film within a month; and because the battery can achieve continuous measurement, and chemical reactions occur during the test, the loss of electrodes and electrolytes cannot be ignored; before measurement, constant calibration is required, once used It takes a long time to replace the electrolyte; while using the electrochemical method to measure the oxygen concentration, the defect of oxygen consumption is still not improved, and this defect still limits the scope of use of the electrochemical method

CN1037968A discloses a multifunctional tissue cell oxygen meter, which is composed of a tissue oxygen measurement chamber, a cell oxygen measurement chamber, a constant temperature water pool, and a sensor. The tissue oxygen measurement chamber and the cell oxygen measurement chamber are designed on one device, which can be used in biological Determination of tissue and cell oxygen consumption, but there is no way to simultaneously measure oxygen and glucose

This method utilizes the assembly driving force of amphiphilic macromolecules to realize one-step co-assembly of macromolecules and carbon nanotubes, and prepares polymer-carbon nanotube hybrids, thereby obtaining a stable enzyme-free glucose sensor. Measurement of sugar sugar and oxygen

Images