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Chinese rose transcription factor RhMYB4 and application thereof to regulation and control of floral organ development

A technology of flower organ, 1.rhmyb4, applied in the field of molecular biology

Active Publication Date: 2019-04-05
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The role of MYB transcription factors in the effect of low temperature on the development of rose flower organs has not yet been clarified

Method used

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  • Chinese rose transcription factor RhMYB4 and application thereof to regulation and control of floral organ development
  • Chinese rose transcription factor RhMYB4 and application thereof to regulation and control of floral organ development
  • Chinese rose transcription factor RhMYB4 and application thereof to regulation and control of floral organ development

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Experimental program
Comparison scheme
Effect test

Embodiment

[0038] The technical solutions of the present invention will be exemplified below in the form of embodiments, but the protection scope of the present invention is not limited to these embodiments.

[0039] 1. Test materials

[0040] Plant material: The material used in the VIGS experiment is the rose variety ‘Samantha’, and its tissue culture seedlings are multiplied by our laboratory. The bimolecular fluorescence complementation test material is wild-type tobacco Nicotiana benthamiana.

[0041] The test strains and vectors are shown in Table 1 below:

[0042] Commonly used strains or plasmids

Model or name

Escherichia coli

TOP10

Agrobacterium

GV3101

Yeast

Y2H GOLD (Gold Yeast), AH109

Cloning vector

pMD18-T

Overexpression vector

Super1300-GFP(C)

VIGS vector

pTRV1, pTRV2

Yeast carrier

pBD-GAL4, pGBKT7, pGADT7 (Clontech)

[0043] Test equipment: PCR instrument, water bath, metal bath, Eppendorf pipette, pH meter, constant temperature incubator, constant tempera...

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Abstract

The invention relates to a Chinese rose transcription factor RhMYB4 and application thereof to the regulation and control of floral organ development. Specifically, the invention provides an RhMYB4 gene; the gene is characterized by comprising coding region nucleotide sequences shown as 263 bp to 1039 bp of SEQ ID NO. 23. The invention further provides protein which is coded by the gene or has anamino acid sequence shown as SED ID NO. 24. The invention further provides application of the gene to the regulation of the floral organ development of plants and a low-temperature-resisting capability. Based on the function of the transcription factor RhMYB4 which is found at present, the gene can be applied to the floral organ development of the plants, such as double flower petals, heteromorphosis of the flower petals, stamen abortion and the regulation of the low-temperature-resisting capability.

Description

Technical field [0001] The present invention relates to the field of molecular biology, in particular to rose transcription factor RhMYB4 and its application in the development and regulation of floral organs. Background technique [0002] Rose (Rosa hybrida) is an evergreen or semi-evergreen shrub in the Rosaceae. It is one of the oldest flowers in the world. It not only has high ornamental value, but also can be used for edible and medicinal purposes. Cut flowers, potted flowers and garden flowers are the main uses of roses, with huge economic value. The average monthly supply of roses in 2017 was more than 60 million. It should be pointed out that the flower type of the cut rose is one of the important ornamental characteristics of the rose, and its damage will seriously reduce the quality of the cut rose and its commercial value. [0003] In the annual production of cut rose flowers, the traditional festivals Spring Festival and Valentine's Day, the demand for roses reaches th...

Claims

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Application Information

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IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/00A01H5/02A01H6/74
CPCC07K14/415C12N15/8218C12N15/827C12N15/8273
Inventor 周晓锋马男李永红李禹琪刘妍妍雷诗瑶
Owner CHINA AGRI UNIV
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