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Nano-selenium bifidobacterium longum as well as preparation method and application thereof

A technology of Bifidobacterium longum and Bifidobacterium, applied in the biological field, can solve the problems of poor improvement of diabetes and poor kidney function, and achieve the effects of reducing renal pathological lesions, preventing diabetic hyperglycemia, and reducing harm

Inactive Publication Date: 2019-04-05
JIANGSU TARGET BIOMEDICINE RES INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the shortcomings in the prior art that the improvement effect of diabetes is not good and it is easy to cause poor kidney function, the purpose of the present invention is to provide a nano-selenium Bifidobacterium longum and its preparation method, and apply it to the preparation of Diabetes progress and improvement of diabetic physiological indicators and metabolic status (fasting blood sugar, body weight, serum insulin level, glucose tolerance test, food intake, water intake and urine output, etc.), improvement of diabetic insulin signaling pathway, improvement of diabetic liver, pancreas and Pathological changes of the kidneys, prevention of diabetic renal complications or renal function damage, drugs or food for improving serum creatinine and blood urea nitrogen levels in diabetes

Method used

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  • Nano-selenium bifidobacterium longum as well as preparation method and application thereof
  • Nano-selenium bifidobacterium longum as well as preparation method and application thereof
  • Nano-selenium bifidobacterium longum as well as preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0044] (1) Preparation of bacteria solution

[0045] Bifidobacterium longum was anaerobically cultured in TPY medium at 37°C: red nano-selenium was dissolved in 200mL of TPY medium with a final concentration of 5g / mL (the concentration of nano-selenium was 1 . :25 was inoculated into new TPY substratum (the mass ratio of tried Bifidobacterium longum bacterium liquid amount and TPY substratum is respectively 1:20,1:22.5,1:25,1:27.5,1:30, its similar effect), put them into a 37°C constant temperature incubator for overnight cultivation until the OD600 value reaches 0.2, and prepare for gavage use the next day; finally, collect the cultured strains, and then wash them with 5% glucose saline for 3 times, and then centrifuge The conditions were 3500 rpm, 4°C, 5 minutes; the collected bacterial solution was resuspended in 0.1 mL of milk, and given to mice for oral gavage. The control group was given 100 μL of 13% skimmed milk per mouse.

[0046] (2) Establishment of diabetic mode...

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Abstract

The invention belongs to the technical field of biology and in particular discloses nano-selenium bifidobacterium longum as well as a preparation method and application thereof. The application of thenano-selenium bifidobacterium longum prepared by the invention to the preparation of medicines or foods for delaying a diabetes mellitus progress comprises: application to medicines or foods for improving physiological index and metabolism conditions of diabetes mellitus, improving pathological changes of diabetes mellitus livers, pancreatic glands and kidneys, improving diabetes mellitus serum creatinine and blood urea nitrogen levels and preventing diabetes mellitus kidney complications or kidney function harms.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a nano-selenium bifidobacterium longum, a preparation method thereof, and an application in preparing medicine or food for delaying the progress of diabetes. Background technique [0002] Bifidobacterium is a genus of bacteria that can grow naturally in the human gastrointestinal tract and protect the host against viral infection. It belongs to the genus of Gram-positive, non-motile, branched-chain anaerobic bacteria. Bifidobacteria have been used as probiotics to aid digestion. Previous studies have shown that Bifidobacterium longum, an anaerobic bacterium, has anticancer effects on liver cancer cells. Several studies have reported a role for probiotics in metabolic disorders including diabetes. [0003] Selenium is an essential trace element required for human and animal health, and it actively participates in animal physiological processes mainly in the form of selenoproteins. ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/745A61P3/10A61P1/16A61P1/18A61P13/12C12R1/01
CPCA61K2035/115A61P1/16A61P1/18A61P3/10A61P13/12C12N1/20
Inventor 华子春林艳张燕徐根兴
Owner JIANGSU TARGET BIOMEDICINE RES INST
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