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Method for identifying agarwood

A kind of agarwood and solution technology, applied in the field of analysis and testing, can solve problems such as incomplete extraction of effective component information, adverse effects on the health of operators, and incomplete chromatographic information, so as to improve detection accuracy and reproducibility, save identification time, The effect of simple and convenient operation

Inactive Publication Date: 2019-03-29
北京三和药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Though the identification method of existing this agarwood uses ethanol as extracting solution, avoids the volatilization of ether, but has the following problems: 1, uses ethanol as extracting solution, and ethanol boiling point is higher, causes ultrasonic time shorter, and the information of effective component Extraction is incomplete; 2, use ethyl acetate and acetone as developer, ethyl acetate and acetone are all irritating, have adverse effects on the health of operators; Not good, the chromatographic information is incomplete

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0046] Embodiment 1: A method for identifying agarwood, including the following steps:

[0047] S1: Preparation of test solution: Take 1g of test agarwood powder, add 20mL of ethanol solution, sonicate for 50min, filter, dilute the filtrate to 25mL, repeat the operation to prepare test solution 1-12;

[0048] Among them, the test agarwood powder particles are 30 meshes, the ultrasonic treatment power is 100W, the frequency is 30KHz, and the temperature is 30℃;

[0049] S2: Preparation of reference medicine solution and reference substance solution:

[0050] Control medicinal material solution: Take 0.5 g of agarwood medicinal material, add 20 mL of ethanol solution, ultrasonicate for 50 min, filter, and dilute the filtrate to 25 mL to prepare a control medicinal material solution;

[0051] Among them, agarwood reference medicinal material is agarwood with batch number 121222-201203 of China Institute for Food and Drug Control, the power of ultrasonic treatment is 100W, the frequency is ...

Embodiment 2

[0054] Embodiment 2: A method for identifying agarwood, including the following steps:

[0055] S1: Preparation of test solution: take 1g of test agarwood powder, add 25mL of ethanol solution, sonicate for 60min, filter, dilute the filtrate to 25mL, repeat the operation to prepare test solution 1-12;

[0056] Among them, the test agarwood powder has 35 meshes, the ultrasonic treatment power is 300W, the frequency is 40KHz, and the temperature is 35℃;

[0057] S2: Preparation of reference medicine solution and reference substance solution:

[0058] Control medicinal material solution: Take 0.5 g of agarwood medicinal material, add 25 mL of ethanol solution, sonicate for 60 min, filter, and dilute the filtrate to 25 mL to prepare a control medicinal material solution;

[0059] Among them, agarwood reference medicinal material is agarwood with batch number 121222-201203 of China Institute for Food and Drug Control, the power of ultrasonic treatment is 300W, the frequency is 40KHz, and the ...

Embodiment 3

[0062] Embodiment 3: A method for identifying agarwood, including the following steps:

[0063] S1: Preparation of test solution: Take 1g of test agarwood powder, add 30mL of ethanol solution, ultrasonically treat for 70min, filter, and dilute the filtrate to 25mL, repeat the operation to prepare test solution 1-12;

[0064] Among them, the test agarwood powder particles are 40 meshes, the ultrasonic treatment power is 500W, the frequency is 50KHz, and the temperature is 40℃;

[0065] S2: Preparation of reference medicine solution and reference substance solution:

[0066] Control medicinal material solution: Take 0.5 g of agarwood medicinal material, add 30 mL of ethanol solution, ultrasonically treat for 70 min, filter, and dilute the filtrate to 25 mL to prepare a control medicinal material solution;

[0067] Among them, agarwood reference medicinal material is agarwood with batch number 121222-201203 of China Institute for Food and Drug Control, the power of ultrasonic treatment is ...

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Abstract

The invention discloses a method for identifying agarwood. The method comprises the following steps of: S1: preparing a test solution: taking 1g of test agarwood powder, adding 20-30 mL of ethanol solution, performing sonicating for 50-70 min, performing filtering, and diluting the filtrate to a volume of 25mL to prepare the test solution; S2: preparing a reference drug solution and a reference substance solution, wherein the reference drug solution is prepared by taking agilawood medicinal materials with 0.5g to perform the step S1 to prepare the reference drug solution, and the reference substance solution is prepared by taking methanol solution of 6,7-dimethyl-2(2-phenethyl)chromone to prepare the reference substance solution containing 0.05mg in each 1mL; and S3: performing thin layerchromatographic detection to absorb each of three solutions for 5[Mu]L which are respectively dispensed on the same silica gel GF254 thin layer plate, taking the methyl alcohol-glacial acetic acid asa developing solvent to perform development, taking-out and drying, and performing viewing under an ultraviolet lamp and the sunlight. Besides, the method provided by the invention is safe to operate,better in reproducibility and more complete in the chromatography information.

Description

Technical field [0001] The invention relates to the technical field of analysis and testing, and more specifically, it relates to a method for identifying agarwood. Background technique [0002] Agarwood is a kind of wood containing resin from the spice plant white wood incense. It is produced in southern China and Southeast Asia. It is a precious wood and also a precious Chinese medicinal material and spice. Since natural agarwood is slow to form incense and expensive, and artificial agarwood is still in the development stage, it is unable to produce high-quality agarwood on a large scale, so it cannot meet the market demand, so it is not uncommon to fake real or shoddy agarwood. Among them, flavored wood or scented low-priced wood pretends to be high-priced agarwood. [0003] One of the identification methods stipulated in the 2015 edition of "Chinese Pharmacopoeia" is thin layer chromatography. The specific method is: take 0.5g of the powder of this product, add 30ml of ether, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90
CPCG01N30/90
Inventor 郭学东陈相银张红莲
Owner 北京三和药业有限公司
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