Molecular marker for identifying fertility of hybrid progeny of common wild rice and cultivated rice and application of molecular marker

A technology of molecular markers and fertility, applied in the biological field, can solve problems such as limiting the effective use of good genes in wild rice

Active Publication Date: 2019-03-29
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the sterility or semi-sterility of wild rice and cultivated rice hybrids and hybrid offspring, the effective use of wild rice genes is limited. Therefore, it is of great significance to identify the fertility of wild rice and cultivated rice hybrids and hybrid offspring.

Method used

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  • Molecular marker for identifying fertility of hybrid progeny of common wild rice and cultivated rice and application of molecular marker
  • Molecular marker for identifying fertility of hybrid progeny of common wild rice and cultivated rice and application of molecular marker
  • Molecular marker for identifying fertility of hybrid progeny of common wild rice and cultivated rice and application of molecular marker

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Embodiment 1

[0053] Example 1. Application of Rice Fertility Molecular Markers in Detecting the Fertility of Offspring of Rice Distant Hybrids

[0054] 1. Molecular markers related to the fertility of rice distant hybrids

[0055] The present invention has discovered a SNP site that can be used to detect the fertility of rice distant hybrid offspring, which is recorded as a rice fertility molecular marker. In rice, the molecular marker is T or C, and the SNP site is corresponding to The 2099th nucleotide of sequence 1 in the sequence listing. The rice whose SNP site is T in both chromosomes is recorded as TT genotype rice, the rice whose SNP site is C in both chromosomes is recorded as CC genotype rice, and the SNP site in Rice with T in one chromosome and C in the other chromosome was designated as TC genotype rice.

[0056] Design the primer pair A1 capable of detecting this site. A1 is composed of two single-stranded DNAs named P1 and P2. The reverse complementary single-stranded DNA...

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Abstract

The invention discloses a molecular marker for identifying fertility of hybrid progeny of common wild rice and cultivated rice and an application of the molecular marker. The rice molecular marker isnucleotide, corresponding to the 2099th position in sequence 1 of a sequence table, in a rice genome, namely, T or C. Experiments prove that the rice molecular marker is related to fertility of distant hybrid progeny of rice, especially fertility of female gametes; in the hybrid progeny of the common wild rice and the cultivated rice, fertility of TT genotype rice with the rice fertility molecularmarkers in two chromosomes being both T and fertility of TC genotype rice with the rice fertility molecular markers being T in one chromosome and C in the other chromosome are lower than the fertility of CC genotype rice with the rice fertility molecular markers in two chromosomes being both C; the female gametes of the TT genotype rice and the TC genotype rice develop abnormally, while female gametes and male gametes of the CC genotype rice develop normally. Therefore, the molecular marker can be used for detecting the fertility of the distance hybrid progeny of the rice, especially the fertility of the female gametes.

Description

technical field [0001] The invention relates to a molecular marker for identifying the fertility of hybrid offspring of common wild rice and cultivated rice in the field of biotechnology and its application. Background technique [0002] Rice production accounts for about 40% of the world's total food production, and increasing rice yield per unit area is of great significance to ensuring world food security. Wild rice has rich genetic variation and contains rich genes that can improve rice yield, quality and stress resistance, and can be used as an excellent germplasm resource for cultivated rice breeding. Discovering and utilizing wild rice germplasm gene resources, and using these favorable genes in wild rice in production will help to break through the bottleneck of further improving rice yield potential. However, due to the sterility or semi-sterility of wild rice and cultivated rice hybrids and hybrid offspring, the effective use of wild rice genes is limited. Therefo...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895
CPCC12Q1/6895C12Q2600/13C12Q2600/172
Inventor 付永彩侯晶晶刘雅馨谭禄宾朱作峰刘凤霞孙传清
Owner CHINA AGRI UNIV
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