Microfluidic PCR chip and operation method thereof

Abstract

The invention provides a microfluidic PCR chip and an operation method thereof. The microfluidic PCR chip comprises a matrix; a fluid control structure consisting of a cavity and a control body is arranged on the matrix. The microfluidic PCR chip also comprises a plurality of cavities constructed on the matrix, the top ends of all the cavities are provided with openings, the cavities are correspondingly connected with channels in the fluid control structure one by one respectively, and each cavity at least comprises a lysate tank loaded with a lysate, a washing buffer solution tank loaded witha washing buffer solution, an eluent tank loaded with an eluent, and a DNase-free water tank loaded with DNase-free water. On the basis of realizing the functions of fluid extraction, mixing and discharge, the microfluidic PCR chip saves the trouble of setting a separated storage or mixing cavity, reduces the operation steps, reduces the complexity of the chip, does not need externally-connectionlarge-scale equipment, reduces the cost of the chip, makes the chip suitable for being used as a disposable microfluidic chip, and has good practicability.

Description

technical field [0001] The invention relates to the field of microfluidic technology in biomolecular detection, in particular to a microfluidic PCR chip, and at the same time, the invention also relates to an operation method of the microfluidic PCR chip. Background technique [0002] In microfluidic chips, especially for microfluidic PCR chips, microvalves and micropumps are important components for controlling fluid flow between reservoirs, microchannels and reaction chambers. With the development of technology, the microfluidic PCR chip is gradually transitioning from a single function to multifunctional integration. Various operations such as liquid flow, liquid mixing, liquid separation, and biochemical reactions performed on the microfluidic PCR chip are mostly Both need micro valve and micro pump structure to cooperate. [0003] At present, the micropump and microvalve on the microfluidic PCR chip have separate pump and valve functions, and some have integrated micro...

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Problems solved by technology

However, these methods have high requirements for peripheral circuit design, high power consumption and low efficiency.

Non-mechanical micropumps focus more on the characteristics of the liquid in the chip, and use methods such as surface tension, electroosmotic force, and magnetohydrodynamics to drive the liquid, which also has the disadvantages of poor versatility and low efficiency.

[0005] Therefore, the existing fluid control structure in the above-mentioned microfluidic PCR chip makes the operation steps of the chip cumbersome, increases the complexity of the chip, and also increases the cost of the chip, but has many shortcomings

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