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Microbial mineralized cementation treatment liquid and sandy slope treatment method

A technology of microorganisms and treatment liquid, which is applied in the treatment of sandy slopes and the field of microbial mineralization cementation treatment liquid, can solve the problems of low survival rate of grass planting, endangering the environment, endangering driving safety, etc., achieving broad application prospects and improving cementing ability. , The effect of preventing erosion damage

Active Publication Date: 2019-03-26
INNER MONGOLIA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] With the rapid development of my country's economy, the number of road construction projects is gradually increasing, and the requirements for construction are getting higher and higher. However, the landslide of the roadbed side slope is one of the common diseases in the roadbed, which blocks traffic and endangers driving safety. Stability has a great impact on the overall performance of the subgrade structure, and also brings serious economic losses to local residents
For many sandy slopes, the cohesion is small, the stability is poor, and it is easy to be washed by rainwater, causing soil erosion and geological disasters such as collapses and landslides, which seriously endanger the environment and easily lead to safety accidents.
[0003] At present, conventional protective measures are mainly adopted, such as geosynthetic material protection, asphalt protection, concrete sash, grass planting and other protective technologies to enhance the overall stability and erosion resistance of the slope soil, but this conventional protective measure has many shortcomings. , For the cold and arid areas in the north, dry and less rain, strong wind and sand, sparse vegetation, strong surface erosion and cutting, low survival rate of grass planting, extremely poor growth conditions, it is difficult to maintain the stability of the roadbed, and cannot achieve a good protective effect

Method used

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  • Microbial mineralized cementation treatment liquid and sandy slope treatment method
  • Microbial mineralized cementation treatment liquid and sandy slope treatment method
  • Microbial mineralized cementation treatment liquid and sandy slope treatment method

Examples

Experimental program
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Effect test

Embodiment 1

[0037] For the control method of sandy and easily eroded sandy slopes of the present invention, please refer to figure 1 , including the following steps:

[0038] Provide a microbial mineralization and cementation treatment liquid for treating sandy slopes;

[0039] First spray the mineralizing bacteria liquid of the microbial mineralization cementation treatment liquid on the sandy slope;

[0040] Then spray the calcium source mixed solution of the microbial mineralization cementation treatment solution on the sandy slope sprayed with the mineralization bacteria solution;

[0041] In this order, the mineralizing bacteria liquid and the calcium source mixed solution are sprayed twice a day, and the time interval between the spraying of the mineralizing bacteria liquid and the calcium source mixed solution is 0.5-1 h each time, and the continuous spraying Five days until the solidified layer on the sandy slope reaches a certain thickness (such as 5cm ~ 10cm), then spray the ...

Embodiment 2

[0054] In this embodiment, the microbial mineralization and cementation treatment solution is composed of a mineralization bacteria treatment solution with mineralization ability and a Bacillus treatment solution with cementation and cohesion ability, and the preparation of the microbial mineralization and cementation treatment solution includes the following steps. Step 1: Prepare the mineralizing bacteria liquid.

[0055] The 2# strain of the mineralizing bacteria is domesticated and cultivated in the culture medium for 3 to 4 times, each time it is connected to the culture medium according to the inoculum amount of 1% to 5%, each time for 24h to 48h, and the culture temperature is 30°C to 30°C. 40°C, 180-200r / min, pH 6.0-6.5, culture time 2d-4d.

[0056] The 2# bacterial liquid culture medium is composed of 12~18g / L yeast powder, 0.05~0.1mol / L NH 4 Cl, 0.1~0.5mol / L CO(NH 2 ) 2 composition.

[0057] Step 2: Prepare calcium source mixed solution.

[0058] The calcium sou...

Embodiment 3

[0072] Mineralizing bacteria (CGMCC No.15633) frozen in a low-temperature refrigerator, see image 3 , and directly poured into the fresh medium (12~18g / L yeast powder, 0.05~0.1molNH 4 Cl, 0.1~0.5molCO(NH 2 ) composition) cultured for 48h, culture temperature 30°C, 200r / min, initial pH value, each time according to the inoculum amount of 1%, inoculated into the new medium, activated culture 3 to 4 times, each 48h, and then according to the required protection For the area of ​​the slope, expand the cultured bacteria solution, and prepare the activated bacteria solution with an OD600 value of 1.0 to 1.5. The main calcium source of calcium source mixed solution is calcium chloride, the concentration of calcium ion is 0.5mol, and 0.2mol~0.35mol NH 4 Cl, 3%~5% Nutrient Broth, 0.5mol~0.8molCO(NH 2 ) 2 , 0.1mol~0.2mol NaHCO 3 and running water.

[0073] The volume ratio of the mineralizing bacteria solution used to the bacillus solution with cementing ability and the treatment...

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Abstract

The invention discloses microbial mineralized cementation treatment liquid and a sandy slope treatment method. The microbial mineralized cementing treatment liquid is formed by mineralized bacteria liquid, bacillus liquid and a calcium source mixed solution which are independent of each other according to the volume ratio of 1:(5-10):(1-3). The mineralized bacteria liquid is divided into 1# bacillus pasteurii liquid and 2# staphylococcus liquid, wherein the 1# bacillus pasteurii liquid is suitable for treating sandy soil with the slope ratio being smaller than 1: 1 and the particle size ranged being smaller than 20 mm, and the 2# staphylococcus liquid is suitable for treating sandy soil with the slope ratio being smaller than 1: 1 and the particle size range d being larger than 0.074 mm and smaller than 10 mm. When the sandy slope is treated, the mineralized bacteria liquid is sprayed on the sandy slope first, then the calcium source mixed solution is sprayed, spraying is carried outtwice a day in the order and carried out for five days continuously, and the bacillus pasteurii liquid is sprayed again after a solidified layer on the sandy slope reaches a certain thickness.

Description

technical field [0001] The present invention relates to a green and environment-friendly slope protection method in the technical field of road slope protection, in particular to a microbial mineralization and cementation treatment solution for treating sandy slopes that are easy to be eroded, and adopts the method The treatment method of the sandy and easy-to-erode sandy slope of the microbial mineralization cementation treatment liquid is described. Background technique [0002] With the rapid development of my country's economy, the number of road construction projects is gradually increasing, and the requirements for construction are getting higher and higher. However, the landslide of the roadbed side slope is one of the common diseases in the roadbed, which blocks traffic and endangers driving safety. The stability has a great influence on the overall performance of the subgrade structure, and also brings serious economic losses to the local residents. For many sandy s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): E02D17/20C12N1/20C12R1/07C12R1/44
CPCC12N1/20E02D17/20
Inventor 李驰高瑜王晓荣范文涵王翠艳
Owner INNER MONGOLIA UNIV OF TECH
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