Application of vibrio halioticoli as feed additive in abalone farming
A technology of feed additive and abalone, which is applied in the field of abalone enterovibrio as a feed additive in abalone farming, to achieve the effect of promoting nutrient absorption
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[0011] one. Isolation and Screening of Enterovibrio WA51 Strain from Abalone
[0012] 1. Source of abalone
[0013] Collected from Dalian sea area.
[0014] 2. Isolation of Enterovibrio WA51 Strain from Abalone
[0015] Dissect and homogenize the intestinal tract of healthy abalone wrinkled under sterile conditions, spread it on 2216E solid medium after gradient dilution, and incubate at a constant temperature of 25°C for 72~96h; pick the strains with the morphology shown in Table 1 to isolate and purify, and then inoculate 2216E The slant medium was stored for later use; the components and proportions of the 2216E medium were: 0.5g peptone, 0.1g yeast extract, 0.001g ferric phosphate, 1000mL seawater, pH 7.2~7.4, sterilized at 121°C for 20min.
[0016] Table 1 Culture characteristics of strains
[0017]
[0018] 3. Screening of Enterovibrio WA51 strain from abalone
[0019] Take the intestinal bacterial strains isolated from the above step 2 preserved in the laborato...
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