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Mesenchymal stem cell preserving fluid for clinic local injection and method for preserving mesenchymal stem cell

A technology of stem cells and local injection, applied in the field of stem cell preservation solution, can solve the problems of low safety and short time to maintain cell viability.

Active Publication Date: 2019-03-26
CHENGDU QINGKE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the above-mentioned deficiencies in the prior art, the present invention provides a clinical mesenchymal stem cell preservation solution and a method for preserving mesenchymal stem cells, which can effectively solve the problem of the low safety of the existing stem cell preservation solution and the time required to maintain cell viability. short question

Method used

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  • Mesenchymal stem cell preserving fluid for clinic local injection and method for preserving mesenchymal stem cell
  • Mesenchymal stem cell preserving fluid for clinic local injection and method for preserving mesenchymal stem cell
  • Mesenchymal stem cell preserving fluid for clinic local injection and method for preserving mesenchymal stem cell

Examples

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Effect test

Embodiment 1

[0033] A mesenchymal stem cell preservation solution for clinical local injection, comprising the following components in volume percentage:

[0034] 3% autologous plasma lysate, 3% autologous stem cell conditioned medium extract, 1% antioxidant vitamin C injection, 0.4% hyaluronic acid, and the rest is physiological saline injection, all of which are clinical injection grade reagents.

[0035] Wherein, the autologous plasma lysate is prepared by the following method:

[0036] (1) Use 8 mL sodium citrate anticoagulant tubes to collect 16 mL of peripheral blood from the patient in 2 tubes;

[0037] (2) Put the collected peripheral blood into a centrifuge and centrifuge at a speed of 1500r / min for 10 minutes. After centrifugation, the peripheral blood is divided into four layers, which are plasma layer, buffy coat layer, and gel layer from bottom to top. Buffy coat layer and blood cell layer, absorb the plasma layer and gently blow the buffy coat layer, then mix the plasma and ...

Embodiment 2

[0044] A mesenchymal stem cell preservation solution for clinical local injection, comprising the following components in volume percentage:

[0045] 1% autologous plasma lysate, 1% autologous stem cell conditioned medium extract, 0.1% antioxidant vitamin C injection, 0.1% hyaluronic acid, and the balance is physiological saline injection.

[0046] The preparation method of autologous plasma lysate and autologous stem cell conditioned medium extract is the same as that in Example 1.

Embodiment 3

[0048] A mesenchymal stem cell preservation solution for clinical local injection, comprising the following components in volume percentage:

[0049] Autologous plasma lysate 5%, autologous stem cell conditioned medium extract 5%, antioxidant vitamin C injection 1%, hyaluronic acid 0.4%, and the balance is physiological saline injection.

[0050] The preparation method of autologous plasma lysate and autologous stem cell conditioned medium extract is the same as that in Example 1.

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Abstract

The invention discloses mesenchymal stem cell preserving fluid for clinic local injection and a method for preserving mesenchymal stem cells. The preserving fluid contains the following components inpercentage by volume: 1%-5% of autologous plasma lysate, 1%-5% of an autologous stem cell conditional medium extract, 0.1%-1% of an antioxidant, 0.1%-0.4% of hyaluronic acid and the balance of a normal saline injection. By preserving the mesenchymal stem cells through the preserving fluid, the cell viability is high, the maintenance time of cell activity is long, and the preserving fluid foes notexogenous component and is high in safety.

Description

technical field [0001] The invention belongs to the technical field of stem cell preservation solution, and in particular relates to a mesenchymal stem cell preservation solution for clinical local injection and a method for preserving mesenchymal stem cells. Background technique [0002] Mesenchymal stem cells (MSCs) are derived from the mesoderm in the early stages of development. MSCs were discovered in the bone marrow in 1968, and they were found to have the characteristics of multidirectional differentiation potential / hematopoietic support and promotion of stem cell implantation / immune regulation and self-replication And increasingly people's attention. [0003] Cell therapy can repair damaged cells, replace necrotic cells, activate dormant cells, and regenerate fresh cells. After stem cells are injected into the human body, they will secrete cytokines, which can promote cell growth. The secreted growth factors bind to aging cell receptors, enhance cell activity, and d...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226
Inventor 高雪华海泉陈静娴赵峻
Owner CHENGDU QINGKE BIOTECH
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