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Novel method for predicting mechanism for treating diabetic nephropathy through gandi capsules on basis of metabolites in hematuria

A technology for diabetic nephropathy and metabolites, applied in the field of metabolomics, can solve problems such as the unclear molecular mechanism of diabetic nephropathy

Active Publication Date: 2019-03-22
XIN HUA HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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Problems solved by technology

However, the molecular mechanism of Gandhi Capsules in treating diabetic nephropathy is still unclear

Method used

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  • Novel method for predicting mechanism for treating diabetic nephropathy through gandi capsules on basis of metabolites in hematuria
  • Novel method for predicting mechanism for treating diabetic nephropathy through gandi capsules on basis of metabolites in hematuria
  • Novel method for predicting mechanism for treating diabetic nephropathy through gandi capsules on basis of metabolites in hematuria

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Experimental program
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Embodiment Construction

[0044] Present embodiment adopts following method to carry out:

[0045] (1) Data collection

[0046] In this embodiment, patients with diabetic nephropathy were taken as subjects, and they were randomly divided into a test group and a control group, with 30 people in each group.

[0047] Among them, the test group: patients with diabetic nephropathy additionally took Gandhi Capsules, 3 times a day, 3 capsules each time, for 6 months continuously.

[0048] Control group: patients with diabetic nephropathy additionally took medicine dregs capsules, 3 times a day, 3 capsules each time, for 6 months continuously.

[0049] 24-h urine and blood samples were collected from patients every 2 months. After blood and urine samples were pretreated, they were chromatographically analyzed using Acquity UPLC BEH C18 column (2.1mm×100mm, 1.7μm, Waters,). The composition of the mobile phase is: B: 0.1% formic acid acetonitrile, the gradient elution settings are as follows: 0-2min: 3% B, 2-...

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Abstract

The invention provides a method for predicting a mechanism for treating diabetic nephropathy through gandi capsules on the basis of metabolites in hematuria. The mechanism is characterized by comprising the following specific steps: S1, selecting an experimental group and a control group according to the ratio of 1: 1; S2, collecting to-be-tested samples at regular intervals; S3, treating the samples in step S2, and carrying out UPLC-Q-TOF / MS separation and analysis; S4, carrying out metabolic profiling analysis on UPLC-Q-TOF / MS atlas data obtained in step S3 to obtain a data set; S5, constructing a PLS-DA model according to the data set obtained in step S4; S6, screening out metabolism markers capable of distinguishing a blank group from a gandi capsule administration group according to an S-PLOT load diagram and a VIP score of the model constructed in step S5; and S7, carrying out enrichment analysis on the metabolism markers which are obtained by screening in step S6 through an IPAanalysis method, and sequencing the metabolism markers according to enrichment efficiency. By the method, the pharmacological mechanism for treating the diabetic nephropathy through the gandi capsulescan be predicted.

Description

technical field [0001] The invention relates to the field of metabolomics, in particular to a method for predicting the mechanism of Gandhi capsules in treating diabetic nephropathy based on metabolites in blood / urine. Background technique [0002] Metabolomics is an important branch of systems biology research. It provides massive information for the interpretation of complex mechanisms through the analysis of biochemical changes and metabolic profiles caused by pathophysiological factors or genetic modifications. The complex pharmacological mechanism coincides with each other, so it can be used in the pharmacological research of Chinese medicine compound preparations. At present, metabolomics research methods have been widely used in the research of the pathological mechanism and drug action mechanism of various metabolic diseases. Nuclear magnetic resonance (NMR), gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS) are three m...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N33/68G01N33/70
CPCG01N30/02G01N30/06G01N33/68G01N33/70G01N2030/065
Inventor 张健徐阿晶陈啸飞张其锵徐人杰王宏宇刘艳祁佳刘悦
Owner XIN HUA HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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