Breeding method for performing physical mutagenesis on coreopsis tinctoria
A technology of chicken chrysanthemum and physics, which is applied in the fields of botanical equipment and methods, plant genetic improvement, application, etc., can solve the problems of small crown width, reduced medicinal value, small leaf area, etc., and achieves improved germination rate and easy operation. , well-structured effect
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[0037] 1 Materials and methods
[0038] 1.1 Materials
[0039] The test material was the seeds of ‘Kunlun Two-color Coreopsis’ from Kunlun Mountains (purchased from Luoyang Gengyuan Agricultural Development Co., Ltd.).
[0040] 2 methods
[0041] Exploration of the imbibition time of seed germination: firstly, through previous experiments, it was known that the seeds were first imbibed in distilled water and then irradiated with ARTP, and the treatment effect was better. Therefore, before using the atmospheric pressure room temperature plasma breeding instrument to irradiate the two-color coreopsis seeds with distilled water for 0h, 6h, 12h, 18h, 24h, 36h and 48h imbibition and water absorption treatment, the number of seeds for each treatment is 20 Then take pictures and count the germination rate on the 4th day after the imbibition treatment, to determine the best imbibition time for seed germination. As a result, it was found that the germination rate was the highest in ...
Embodiment 2
[0067] Carried out in the same manner as in Example 1, the difference is that 50 seeds were used each time, irradiated for 22.50 minutes, and the experiment was repeated 5 times independently, and the same method was used to measure germination potential, germination rate, leaf area, flower diameter and T t Mutagenesis rate, the results are shown in Table 5 below. As can be seen from the results of Table 5, in five repeated tests, the germination rate can be stabilized at more than 80%, and the leaf area is at 170mm 2 Above, the crown width is above 45mm, T t Mutation rate is not less than 8%.
[0068] Table 5. Effect of ARTP on germination rate, leaf area, crown width and T after 22.50 minutes of irradiation t The effect of mutagenesis
[0069] frequency
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