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Method of identifying whether rape honey is mixed in bee honey

A technology for rapeseed honey and honey is applied in the field of food inspection, which can solve the problems of poor stability and low judgment, and achieve the effects of low cost, high judgment and convenient operation.

Active Publication Date: 2019-03-19
新疆新兴源农业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods have poor stability and low judgment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A middleman evaluates the quality of collected raw monofloral honey.

[0032] Operation method:

[0033] ① Accurately weigh 20g single flower honey sample, put it in a dry bottle, add 80ml ultrapure water to dissolve;

[0034] ②Adjust the pH to 6-7 with 5% ammonia water;

[0035] ③ Centrifuge at 10,000×g for 10 minutes, and take the supernatant for later use;

[0036] ④ Reversed-phase-anion exchange solid-phase extraction column (RP-AE SPE) was activated by 1mL methanol and equilibrated with 1mL ultrapure water (pH: 6-7), and the supernatant was passed through the column;

[0037] ⑤ Wash the extraction column with 1ml of ultrapure water, elute the extraction column with 2ml of methanol (containing 10% formic acid), and collect the eluent;

[0038] ⑥ The eluate was blown to dryness with nitrogen at room temperature, redissolved and mixed with 2 mL of chromatographic grade acetic acid methanol solution (2:98 V / V), and passed through a 0.22 μm filter membrane to obtain ...

Embodiment 2

[0045] An enterprise evaluates the quality of the purchased raw material acacia honey.

[0046] Operation method:

[0047] ① Accurately weigh 20g of acacia honey single flower honey sample, put it in a dry bottle, add 80ml of ultrapure water to dissolve;

[0048] ②Adjust the pH to 6-7 with 5% ammonia water;

[0049] ③ Centrifuge at 10,000×g for 10 minutes, and take the supernatant for later use;

[0050] ④ Reversed-phase-anion exchange solid-phase extraction column (RP-AE SPE) was activated by 1mL methanol and equilibrated with 1mL ultrapure water (pH: 6-7), and the supernatant was passed through the column;

[0051] ⑤ Wash the extraction column with 1ml of ultrapure water, elute the extraction column with 2ml of methanol (containing 10% formic acid), and collect the eluent;

[0052] ⑥ The eluate was blown to dryness with nitrogen at room temperature, redissolved and mixed with 2 mL of chromatographic grade acetic acid methanol solution (2:98 V / V), and passed through a 0.22...

Embodiment 3

[0060] A third-party testing agency conducts quality testing on the commissioned commodity rapeseed honey.

[0061] Operation method:

[0062] ① Accurately weigh 20g of linden honey sample, place it in a dry bottle, add 80ml of ultrapure water to dissolve;

[0063] ②Adjust the pH to 6-7 with 5% ammonia water;

[0064] ③ Centrifuge at 10,000×g for 10 minutes, and take the supernatant for later use;

[0065] ④ Reversed-phase-anion exchange solid-phase extraction column (RP-AE SPE) was activated by 1mL methanol and equilibrated with 1mL ultrapure water (pH: 6-7), and the supernatant was passed through the column;

[0066] ⑤ Wash the extraction column with 1ml of ultrapure water, elute the extraction column with 2ml of methanol (containing 10% formic acid), and collect the eluent;

[0067] ⑥ The eluate was blown to dryness with nitrogen at room temperature, redissolved and mixed with 2 mL of chromatographic grade acetic acid methanol solution (2:98 V / V), and passed through a 0....

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Abstract

The invention provides a method of identifying whether rape honey is mixed in bee honey and belongs to the technical field of food detection. The method includes detecting the content of methyl syringate in single-flower bee honey under identification; if the content is greater than 10 mu g / 100 g, indicating that rape honey is mixed in the single-flower bee honey under identification; if the content if less than 10 mu g / 100 g, indicating that no rape honey is mixed in the single-flower bee honey under identification. The method has the advantages that detection results are reliable and stable,judgment degree is high, operation is convenient, and the cost is low.

Description

technical field [0001] The invention belongs to the technical field of food inspection, and in particular relates to a method for identifying whether honey is mixed with rapeseed honey. Background technique [0002] As a natural sweetener, honey is deeply loved by consumers. According to whether the honey comes from a single nectar source plant, honey can be divided into single flower honey and mixed flower honey. Single flower honey is usually named after the flower name, such as acacia nectar named acacia honey. my country is rich in unifloral honey source plants, and there are many types of unifloral honey, such as acacia honey, linden honey, jujube honey, litchi honey, longan honey, rapeseed honey and so on. Honey adulteration often occurs due to the different market prices of each type of unifloral honey. That is to say, the single flower honey with low price is mixed into the honey with high price, and the high price is filled with low price. Rapeseed honey is one o...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 张红城乔江涛
Owner 新疆新兴源农业有限公司
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