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Hypoglycemic peptide and application thereof

A technology for hypoglycemia and diabetes, applied in the biological field, can solve problems such as hypoglycemia, serious adverse reactions, and inconvenient administration

Pending Publication Date: 2019-03-12
NANJING UNIV OF FINANCE & ECONOMICS +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many deficiencies in traditional antidiabetic drugs: the most common treatment method is insulin injection, but its administration is inconvenient, and it is easy to cause hypoglycemia after injection; the adverse reactions of metformin drugs are serious

Method used

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  • Hypoglycemic peptide and application thereof
  • Hypoglycemic peptide and application thereof
  • Hypoglycemic peptide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 Simulated gastrointestinal digestion to reduce the stability of glycopeptides

[0040] Simulated gastrointestinal digestion experiment: Accurately weigh 3 mg of hypoglycemic peptide (amino acid sequence shown in SEQ ID NO: 1), dissolve it in 100 ml of deionized water, adjust the pH of the solution to 2.0 with 1 mol / L hydrochloric acid; add 0.12 mg of pepsin After stirring, incubate in a constant temperature shaker at 37°C for 2h; heat the sample in a boiling water bath for 10min to inactivate the enzyme. Then adjust the pH of the solution to 7.5 with 1 mol / L sodium hydroxide solution; add 0.12 mg of trypsin, stir moderately and incubate in a constant temperature shaker at 37°C for 2 hours, and finally heat in a boiling water bath for 10 minutes to inactivate the enzyme.

[0041] The hypoglycemic peptide (denoted as LY-1) after the simulated gastrointestinal digestion experiment was detected by high performance liquid chromatography, and the untreated hypoglyce...

Embodiment 2

[0043] Example 2 Glucose Consumption Test of Smooth Muscle Cells

[0044] Smooth muscle cells (VSMC) in the logarithmic growth phase were taken, and 8×10 4 Cells / mL were seeded in a 96-well plate at a seeding volume of 100 μL / well. After the cells grew adherently, the supernatant was discarded. Divide VSMC cells into three groups: the first group (blank group, abbreviated as NG), add 100 μL / well of low-glucose medium; the second group (model group, abbreviated as HG), add high-glucose medium, 100 μL / well ; The third group (experimental group) set 4 groups of different conditions, respectively added high glucose culture solution containing 5 μM, 10 μM, 20 μM, 40 μM hypoglycemic peptide (sequence shown in SEQ ID NO: 1), 100 μL / well; Six replicate wells were set up for each concentration. Among them, the low-sugar culture medium is DMEM medium supplemented with 10% serum (glucose concentration is 5 mM), and the high-sugar culture medium is DMEM medium supplemented with 10% seru...

Embodiment 3

[0046] Example 3 Effects of Hypoglycemic Peptides on Diabetic Mice

[0047] 1. Modeling method of diabetic mice

[0048] This paragraph describes the method for modeling diabetic mice. Thirty C57BL6 male mice, each weighing 20±2g, were provided by Wuxi Shanhe Pharmaceutical Company. Feed with common feed for 1 week to adapt to the environment. After that, 10 mice in each group (fed separately, 5 mice / cage), were randomly divided into 3 groups: normal control group (marked as WT), STZ-induced model group (marked as STZ), experimental group (marked as for STZ+AOP). The STZ-induced model group and the experimental group received intraperitoneal injection of STZ (streptozotocin), 50 mg / kg, for 5 days; the normal control group received the same amount of sodium citrate injection. Seven days after the end of STZ administration, fasting for 12 hours, the tail blood was taken to test the fasting blood glucose of the mice. It was found that the blood glucose values ​​of the mice in...

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Abstract

The invention provides a hypoglycemic peptide with the amino acid sequence ADWGGPLPH shown as SEQ ID NO:1 and further provides a modified peptide of the hypoglycemic peptide. Chemical groups, amino acids, polypeptides, proteins or PEG is connected to an N terminal, a C terminal or intermediate residues of the hypoglycemic peptide. Through the hypoglycemic peptide, glucose absorption of smooth muscle cells under the high-glucose condition can be promoted, the glucose tolerance of diabetic mice can be improved, the blood glucose utilization ability of the mice can be enhanced, serum total cholesterol and total triglycerides are significantly reduced, the SOD level is significantly improved, the MDA level is lowered, the oxidative injury of the livers of the diabetic mice is repaired, inflammatory factors in plasma of the diabetic mice are inhibited, and the hypoglycemic peptide can resist metabolic syndromes and can be used for preparing drugs or health care products for treating and / orpreventing diabetes or hyperlipidemia.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a hypoglycemic peptide and its application. Background technique [0002] Diabetes mellitus (DM) is a metabolic disorder disease characterized by relative or absolute insulin deficiency and hyperglycemia as the main symptom. Insufficient insulin secretion or related function blockages lead to elevated blood sugar, leading to metabolic disorders of the body and ultimately affecting normal physiology. Function and cause complications such as atherosclerosis, cataract and pancreatitis. Common diabetes is divided into type 1 diabetes and type 2 diabetes. There are many deficiencies in traditional antidiabetic drugs: the most common treatment method is insulin injection, but its administration is inconvenient, and it is easy to cause hypoglycemia after injection; the adverse reactions of metformin drugs are serious. Contents of the invention [0003] The invention provides...

Claims

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Application Information

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IPC IPC(8): C07K14/415A61K38/16A61P3/10A61P3/06A23L33/185A23L33/18
CPCA23V2002/00A61K38/00A23L33/18A23L33/185A61P3/06A61P3/10C07K14/415A23V2200/3262A23V2200/328
Inventor 沈新春汪芳吕奕翁泽斌刘永祥汤晓智方勇
Owner NANJING UNIV OF FINANCE & ECONOMICS
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