A kind of antioxidative peptide of columnar Agrocybe edodes and its use

A technology of columnar field head mushroom and antioxidant peptide, which is applied in the biological field to achieve the effects of large yield, high purity and increasing added value of products

Active Publication Date: 2021-09-10
武汉华裕新美菌业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few literature reports on the antioxidant peptides of Amanitula cylindrica

Method used

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  • A kind of antioxidative peptide of columnar Agrocybe edodes and its use
  • A kind of antioxidative peptide of columnar Agrocybe edodes and its use
  • A kind of antioxidative peptide of columnar Agrocybe edodes and its use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1) Weigh 1 kg of fruiting body powder of Amanitatus cylindrica, add 10 times the weight of water, extract at 60°C for 4 hours, add saturated ammonium sulfate to the extract after centrifugation for salting out, and the saturation of the final concentration of ammonium sulfate is 80%. dissolving the salted-out precipitate with water, then dialyzing through a dialysis bag with a molecular weight cut-off of 4000 Da, and drying the dialyzed precipitate to obtain the columnar Agrocybe edodes crude protein;

[0031] 2) Add 35 times the weight of water to the crude protein, adjust the pH to 8.0, then add 3% neutral protease (specific activity is 20,000 international units) by weight, enzymolyze at 50°C for 6 hours, and then boil to inactivate Centrifuge to collect the filtrate;

[0032] 3) The filtrate is first microfiltered through a 0.22 μm filter membrane, and then ultrafiltered with a 3500 Dalton ultrafiltration membrane to collect the ultrafiltrate;

[0033] 4) The ultr...

Embodiment 2

[0035] 1) Weigh 1 kg of fruiting body powder of Amanitatus cylindrica, add 20 times the weight of water, stir evenly, extract at 80°C for 3 hours, add saturated ammonium sulfate to the extract after centrifugation for salting out, the final concentration of ammonium sulfate Saturation is 80%. Dissolving the precipitate after salting out with water, then dialyzing through a dialysis bag with a molecular weight cut-off of 3000Da, drying the precipitate after dialysis, to obtain the crude protein of Amanitula edodes;

[0036] 2) Add 25 times the weight of water to the crude protein, adjust the pH to 7.5, then add 4% neutral protease by weight, enzymatically hydrolyze at 60°C for 5 hours, then boil and inactivate, then centrifuge, and collect the filtrate;

[0037] 3) The filtrate is first microfiltered through a 0.22 μm filter membrane, and then ultrafiltered with a 3500 Dalton ultrafiltration membrane to collect the ultrafiltrate;

[0038] 4) The ultrafiltrate was further separ...

Embodiment 3

[0040] 1) Weigh 1 kg of fruiting body powder of Amanitatus cylindrica, add 6 times the weight of water, stir evenly, extract at 65°C for 5 hours, add saturated ammonium sulfate to the extract after centrifugation for salting out, the final concentration of ammonium sulfate Saturation is 80%. dissolving the salted-out precipitate with water, then dialyzing through a dialysis bag with a molecular weight cut-off of 4000 Da, and drying the dialyzed precipitate to obtain the columnar Agrocybe edodes crude protein;

[0041] 2) Add 50 times the weight of water to the crude protein, adjust the pH to 8.5, then add 2% neutral protease by weight, enzymatically hydrolyze at 55°C for 10 hours, then boil and inactivate, then centrifuge, and collect the filtrate;

[0042] 3) The filtrate is first microfiltered through a 0.22 μm filter membrane, and then ultrafiltered with a 3500 Dalton ultrafiltration membrane to collect the ultrafiltrate;

[0043] 4) The ultrafiltrate was further separated...

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Abstract

The invention discloses an anti-oxidation peptide of a cylindrical Amanita edulis. The anti-oxidation peptide contains a peptide segment whose amino acid sequence is PAQPPPPPNR. The invention also discloses a preparation method of the antioxidant peptide. The present invention is the first to isolate a biologically active peptide from the cylindrical mushroom, and its antioxidant activity has been verified, which can be used as an additive to prepare health care products, food, cosmetics and daily chemical products. The antioxidant peptide has the advantages of high purity and large yield, realizes the deep processing and utilization of columnar mushrooms, and increases the added value of the product.

Description

technical field [0001] The invention belongs to the field of biology, and relates to an anti-oxidation peptide of cylindrical acromantia and its application. Background technique [0002] At present, people have isolated and prepared a variety of biologically active peptides from animals, plants, and microorganisms. These active peptides have various physiological functions, such as anti-oxidation, lowering blood lipids, and inhibiting viruses. Among them, antioxidant peptides are peptides with specific amino acid sequences that are released from some original hidden protein molecules, and achieve antioxidant effects by scavenging oxygen free radicals, hydroxyl free radicals, etc. Compared with synthetic antioxidants, its Higher security, it can not only prevent oxidation in the food system, but also prevent the damage of free radicals in the human body, and delay various degenerative diseases caused by aging, such as cancer, coronary heart disease, diabetes, etc. [0003] ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06C07K1/30C07K1/34C07K1/16C07K1/14C07K1/36
CPCC07K7/06
Inventor 陈义杰沈谦宋蓉梁天鑫丁爽罗依伦周麒
Owner 武汉华裕新美菌业有限公司
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