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Micro-filtration membrane and method for modifying same

A modification method and microfiltration membrane technology, applied in the field of membrane modification, can solve problems such as difficulty in cell release, and achieve the effect of improving the release rate and enhancing the ability of grading screening.

Active Publication Date: 2019-03-08
BEIJING MECHANICAL EQUIP INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the above analysis, the present invention aims to provide a microfiltration membrane and a modification method to solve the problem that the existing microfiltration membrane is difficult to release cells after enrichment

Method used

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preparation example Construction

[0059] (1) Preparation of temperature-sensitive water-soluble polymer solution. In an environment higher than the minimum critical solution temperature of the temperature-sensitive water-soluble polymer, after preparing a high-concentration polymer aqueous solution, according to different application scenarios, dilute to a concentration of 0.1% to 1% by volume, and reserve (low-concentration temperature-sensitive water-soluble polymer solution) The permanent polymer solution has almost no effect on the pore size of the filter membrane);

[0060] (2) Modification of microfiltration membrane. The prepared or purchased microfiltration membrane has a pore size ranging from R1 to R2. A custom-concentrated temperature-sensitive water-soluble polymer solution is applied to the microfiltration membrane by direct immersion or spraying. Place the treated microfiltration membrane at room temperature or 50°C to fully dry it for later use. Since the concentration of the selected polymer ...

Embodiment 1

[0073] There are two cell populations in the cell system, the average size of the A cell population is 6 μm (such as red blood cells), the average size of the B cell population is 15 μm (such as A549 cells), and the target to be enriched is the B cell population. The material of the microfiltration membrane is parylene with a pore size of 8 μm.

[0074] Poly-N-isopropylacrylamide (PNIPAM) was prepared as a solution with a concentration of 0.1% by volume; the microfiltration membrane was immersed in the solution for 1 min, and then taken out to dry naturally. And put the treated microfiltration membrane into the filter device. Inject the mixed cell solution into the upper part 5 of the filter device. After the liquid passes through completely, transfer the microfiltration membrane to a container filled with phosphate buffered saline solution, heat it in a water bath at 34°C, and after 1 min, the release phase ends, and take out the filter membrane , the cell group B is release...

Embodiment 2

[0076] There are two cell populations in the cell system, the average size of the A cell population is 6 μm (such as red blood cells), the average size of the B cell population is 15 μm (such as A549 cells), and the target to be enriched is the B cell population. The material of the microfiltration membrane is parylene with a pore size of 8 μm.

[0077] Poly-N-isopropylacrylamide (PNIPAM) was prepared as a solution with a concentration of 1% by volume; the microfiltration membrane was immersed in the solution for 1 min, and then taken out to dry naturally. And put the treated microfiltration membrane into the filter device. Inject the mixed cell solution into the upper part 2 of the filter device. After the liquid has completely passed through, transfer the microfiltration membrane to a container filled with phosphate buffered saline solution and heat it in a water bath at 34°C. After 1 min, the release phase ends and the filter membrane is taken out. , at this time, the cell...

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Abstract

The invention relates to a micro-filtration membrane and a method for modifying the same, and belongs to the technical field of membrane modification. By the aid of the micro-filtration membrane and the method, the problem of difficulty in releasing enriched cells by micro-filtration membranes in the prior art can be solved. The micro-filtration membrane is modified by temperature-sensitive water-soluble polymer solution. The method for modifying the micro-filtration membrane includes (1), preparing the temperature-sensitive water-soluble polymer solution with the volume percent concentrationof 0.1%-6%; (2), coating the temperature-sensitive water-soluble polymer solution on the micro-filtration membrane to modify the micro-filtration membrane and drying the micro-filtration membrane coated with the temperature-sensitive water-soluble polymer solution to obtain a modified micro-filtration membrane. The micro-filtration membrane and the method have the advantages that membrane filtration technologies can be widely applied to the field of medical treatment, and accurate detection results can be obtained.

Description

technical field [0001] The invention relates to the technical field of membrane modification, in particular to a microfiltration membrane and a modification method. Background technique [0002] Circulating tumor cells (circulating tumor cells, CTCs) and circulating tumor microemboli (circulating tumor microemboli, CTMs) in peripheral blood are considered to be important causes and markers of tumor metastasis and recurrence. [0003] Currently, the membrane filtration technique based on morphological enrichment (isolation by size of epithelial tumor cell, ISET) is one of the main methods for detecting CTC and CTM. Compared with the specific enrichment detection technology that relies on tumor-related markers (such as: EpCAM forward enrichment method), ISET technology is not affected by the down-regulation of specific markers on the surface of tumor cells, and the probability of detecting false negative results is low , and has the advantages of simple operation method, low ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D67/00B01D65/00B01D61/14
CPCB01D61/147B01D65/00B01D67/0088
Inventor 王一凡王忠晶刘立滨许诺臧金良
Owner BEIJING MECHANICAL EQUIP INST
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