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DNA nano tripod-based molecular logic gate for regulating interaction between fluorescent small molecule and graphene oxide and construction method thereof

A tripod and logic gate technology, applied in the field of molecular logic gates and their construction, can solve problems such as increasing the cost of constructing logic gates, scattered logic device structures, and redundant circuits.

Inactive Publication Date: 2019-02-26
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Such a design method leads to the lack of versatility of functional components, which makes the structure of the constructed logic device scattered and the circuit is redundant, which hinders the cascading of basic logic gates into multi-level complex logic circuits, and increases the cost of building logic gates.

Method used

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  • DNA nano tripod-based molecular logic gate for regulating interaction between fluorescent small molecule and graphene oxide and construction method thereof
  • DNA nano tripod-based molecular logic gate for regulating interaction between fluorescent small molecule and graphene oxide and construction method thereof
  • DNA nano tripod-based molecular logic gate for regulating interaction between fluorescent small molecule and graphene oxide and construction method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Using DNA nano-tripod to regulate the interaction between the loaded fluorescent group ROX and graphene oxide, a majority logic gate was constructed.

[0062] Take 8 EP tubes, numbered 1, 2, 3, 4, 5, 6, 7 and 8, and add 20 μL DNA nanotripod stock solution with a concentration of 200 nM, 50 μL H 2 O and 10 μL of a graphene oxide aqueous solution with a concentration of 200.0 ug / mL that were pre-sonicated, mixed, and incubated at room temperature for 10 min.

[0063] Then, no signal input chain is added or the signal input chains I1, I2 and I3 are added separately or in pairs or all are added, and the single-chain leg of the tripod is used as a signal receiving channel to receive the corresponding signal input chain and hybridize with it Form double strands and desorb from the surface of graphene oxide, so that the state of the DNA nano-tripod on the surface of graphene oxide is different, thereby regulating the interaction between the fluorescent groups loaded on it and ...

Embodiment 2

[0071] Construction of two-input AND logic gates and OR logic gates

[0072] Take 4 EP tubes, numbered 1, 2, 3 and 4 respectively, add 20 μL DNA nanotripod stock solution with a concentration of 200 nM, 50 μL H 2 O and 10 μL of a graphene oxide aqueous solution with a concentration of 200.0 ug / mL that were pre-sonicated and dispersed, mixed evenly, and incubated at room temperature for 10 min, and then performed the following treatments.

[0073] Add 20 μL HO to tube 1 2 O, mix well; add 4 μL of I2 chain at a concentration of 5 μM and 16 μL of HO to tube 2 2 O, mix well; add 4 μL of I3 chain at a concentration of 5 μM and 16 μL of HO to tube 3 2 O, mix well; add 4 μL of I2 chain at a concentration of 5 μM, 4 μL of I3 chain at a concentration of 5 μM, and 12 μL of HO into tube 4 2 O, mix well.

[0074] Place each sample tube in a biochemical incubator and incubate at 37°C for 30min.

[0075] Take 95 μL of sample and transfer it to a cuvette to measure the fluorescence of t...

Embodiment 3

[0087] Construction of INHIBIT logic gates

[0088] Take 4 EP tubes, numbered 1, 2, 3 and 4 respectively, add 20 μL DNA nanotripod stock solution with a concentration of 200 nM, 4 μL I2 chain with a concentration of 5 μM, 46 μL H 2 O and 10 μL of a graphene oxide aqueous solution with a concentration of 200.0 ug / mL that had been pre-sonicated, mixed evenly, and incubated at room temperature for 10 min, and then performed the following treatments.

[0089] Add 20 μL HO to tube 1 2 O, mix well; add 4 μL of I1 chain at a concentration of 5 μM and 16 μL of HO to tube 2 2 O, mix well; add 4 μL of 5 μM I1’ chain and 16 μL of H to tube No. 2 O, mix well; add 4 μL of I1 chain at a concentration of 5 μM, 4 μL of I1’ chain at a concentration of 5 μM, and 12 μL of H 2 O, mix well.

[0090] Place each sample tube in a biochemical incubator and incubate at 37°C for 30min.

[0091] Take 95 μL of sample and transfer it to a cuvette to measure the fluorescence of the sample at room tempe...

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Abstract

The invention discloses a DNA nano tripod-based molecular logic gate for regulating interaction between a fluorescent small molecule and graphene oxide and a construction method thereof. In the self-assembled DNA nanotripods, one central strand is hybridized to form a central triangle by three side strands, the rest of the three side strands are extended to form a single stranded DNA leg as a signal receiving strand, and the central strand is loaded with a fluorescent group ROX. The signal receiving chain is adsorbed on the surface of graphene oxide when it is not hybridized with the signal input chain, and the signal receiving chain is desorbed from the surface of graphene oxide after hybridization with the signal input chain into double strands. DNA nano tripod and graphene oxide affinity are regulated by combining input strand of different signals, The different configurations of DNA nano-tripods on the surface of graphene oxide can regulate the interaction between the supported fluorescent group ROX and graphene oxide to generate different fluorescent signals and realize the construction of a variety of molecular logic gates.

Description

technical field [0001] The invention belongs to the technical field of molecular computers, and in particular relates to a molecular logic gate for regulating the interaction between fluorescent small molecules and graphene oxide based on a DNA nano-tripod and a construction method thereof. Background technique [0002] In modern electronic computers, electronic logic gates are the basic building blocks of integrated circuits. Molecular logic gates are similar to electronic logic gates. With proper design, Boolean logic operation rules can also be applied to molecular reaction systems to realize the conversion from input signals to output signals. Molecular logic gates can convert chemical, biological, and physical input signals into certain output signals. Therefore, molecular logic gates are the basis for building molecular computers. Constructing a molecular logic operation system, using chemical or biochemical reactions to simulate electronic circuit systems for logic o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G06N99/00
CPCG06N99/007
Inventor 何开雨徐霞红王柳何红梅吴珉王强
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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