Method for detecting fatty acid content of nematode by gas chromatography and mass spectrometry combined technology
A technology of gas chromatography and combined technology, which is applied in the field of gas chromatography and mass spectrometry to detect the content of fatty acids in nematodes, can solve the problems of long response time, low sensitivity, and long time consumption, and achieve low detection limit, high sensitivity, and consumption. short-term effect
Inactive Publication Date: 2019-02-26
JIANGHAN UNIVERSITY
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[0005] The embodiment of the present invention provides a method for detecting the content of fatty acids in nematodes by gas chromatography and mass spectrometry, so as to solve the problems of long time-consuming, relatively poor results, long response time and low sensitivity of the existing detection and analysis methods. Analysis of fatty acid composition and content in nematodes
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[0029] In order to make the object, technical solution and advantages of the present invention clearer, the implementation manner of the present invention will be further described in detail below in conjunction with the accompanying drawings.
[0030] Detection instrument: chromatography system: TRACE 1300 series modular gas chromatograph (Thermo Fisher, USA); mass spectrometry system: ISQ TM LT single quadrupole GC-MS system (Thermo Fisher, USA)
[0031] Instrument testing conditions:
[0032] Chromatographic conditions
[0033] Chromatographic column: DB-WAX (30m×0.25mm×0.25μm, Agilent, USA);
[0034] Carrier gas: high-purity helium, 1.0mL / min, constant flow rate;
[0035] Injection method: split injection, split ratio 50:1; injection volume 1μL;
[0036] Injection port temperature: 220°C;
[0037] Programmed temperature rise: the initial temperature is 50°C, keep for 1min; then raise the temperature to 180°C at a rate of 20°C / min, keep for 5min; finally raise the tem...
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Abstract
The invention discloses a method for detecting the fatty acid content of a nematode by a gas chromatography and mass spectrometry combined technology, and belongs to the technical field of analyticalchemistry. The method comprises the following steps: (1), preparing a standard product, detecting by a gas chromatography and mass spectrometry combined method, and comparing with a mass spectrometrydatabase to obtain fatty acid components corresponding to different retention times, wherein when the similarity is higher than or equal to 95%, a compound is confirmed; (2), preparing a standard mixed solution, and establishing a gas chromatography and mass spectrometry combined qualitative and quantitative method by an internal standard method; (3), pretreating a nematode sample to obtain an analytical sample; (4), performing analytical detection on the analytical sample to be detected by the gas chromatography and mass spectrometry combined qualitative and quantitative method established inthe step (2); (5), determining the content of each fatty acid in the nematode sample through calculation. The detection method is high in detection speed, low in detection limit and high in sensitivity, and is suitable for rapid analysis of the fatty acid composition and the fatty acid content of the nematode.
Description
technical field [0001] The invention belongs to the technical field of analytical chemistry, and in particular relates to a method for detecting fatty acid content in nematodes using gas chromatography and mass spectrometry. Background technique [0002] Compared with other experimental animals, the life cycle of nematodes is very short, which is convenient for periodic observation; the culture conditions are very simple, suitable for laboratory culture; the size is small, the reproduction amount is large, and the needs of experimental samples are guaranteed; and it is a complex regulation. A multicellular eukaryote means that it may have similar cellular and molecular structures and control pathways to higher animals. Because of its unique biological characteristics, it is widely used in the research of life medicine and is an excellent model organism. [0003] The polyunsaturated fatty acid synthesis pathway in nematodes combines the processes of plants and animals. Compa...
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IPC IPC(8): G01N30/02G01N30/06G01N30/72
CPCG01N30/02G01N30/06G01N30/72G01N2030/062G01N2030/025
Inventor 周珍黄子纯梁勇蒋琳
Owner JIANGHAN UNIVERSITY
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