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Microbial activity color development enhancement method for petroleum corrosivity microbial detection kit

A technology for microbial detection and microbial activity, applied in the field of microbial detection, can solve the problems of medium becoming sour, accumulating acidic metabolites, and reducing the color of reduced products, etc., to achieve the effect of increasing sensitivity and enhancing color

Pending Publication Date: 2019-02-22
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, during the growth of microorganisms in the detection medium, acidic metabolites will accumulate, and the medium will also become acidic, resulting in the desalination of the color of the reduced product

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] This implementation case shows a method for enhancing the color development of microbial activity in a petroleum corrosive microbial detection toolbox according to the following steps:

[0027] The microorganisms were grown on a solid medium containing MTT at a pH of 5.0 to 6.5 for three days to produce reduced products of MTT. The alkaline solution used for spraying is a deionized aqueous solution of 10g / L sodium hydroxide, and its pH value is greater than 11. The alkaline solution is stored in a clean plastic watering can of about 100mL. The plastic watering can can spray aerosol droplets to spread on the entire surface of the solid medium, spray twice, each time lasts about 1 second, and the spray volume is 2-5mL. After waiting for 3 to 5 minutes, observe the color development on the medium.

Embodiment 2

[0029] This implementation case shows a method for enhancing the color development of microbial activity in a petroleum corrosive microbial detection toolbox according to the following steps:

[0030]The microorganisms were grown on a solid medium containing MTT at a pH of 4.0 to 5.5 for three days to produce reduced products of MTT. The alkaline solution used for spraying is distilled aqueous solution of 20g / L sodium carbonate, and its pH value is greater than 12. The alkaline solution is stored in a clean plastic watering can of about 100mL. The plastic watering can can spray aerosol droplets to spread on the entire surface of the solid medium, spray twice, each time lasts about 1 second, and the spray volume is 2-5mL. After waiting for 1 to 5 minutes, observe the color development on the medium.

Embodiment 3

[0032] This implementation case shows a method for enhancing the color development of microbial activity in a petroleum corrosive microbial detection toolbox according to the following steps:

[0033] The microorganisms were grown on a solid medium containing MTT at a pH of 5.0 to 7.0 for three days to produce reduced products of MTT. The alkaline solution used for spraying is distilled aqueous solution of 50g / L sodium bicarbonate, and its pH value is greater than 12. The alkaline solution is stored in a clean plastic watering can of about 250mL. The plastic watering can can spray aerosol droplets to spread on the entire surface of the solid medium, spray twice, each time lasts about 2 seconds, and the spray volume is 2-5mL. After waiting for 10-15 minutes, observe the color development on the culture medium.

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PUM

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Abstract

The invention discloses a microbial activity color development enhancement method for a petroleum corrosivity microbial detection kit. The method includes spraying an alkaline solution to the surfaceof a solid medium of a formazan reduzate containing red tetrazolium or blue tetrazolium after microorganisms grow on the solid medium. According to the method, a user can observe the color of the reduzate of the pigments on the solid medium with the naked eyes, so that the number and the activity degree of microbial colonies on the solid medium can be observed and judged more sensitively with thenaked eyes.

Description

technical field [0001] The invention belongs to the technical field of microorganism detection, and in particular relates to a method for enhancing color development of microorganism activity for a petroleum corrosive microorganism detection toolbox. Background technique [0002] Petroleum or jet fuel storage can trigger corrosion in large storage tanks. According to reports, this type of corrosion is mainly caused by microbial growth. Corrosive microorganisms in oil plants mainly include three types: bacteria, fungi and actinomycetes. Some microorganisms are particularly corrosive, such as sulfate reducing bacteria (SRB), sulfur oxidizing bacteria and iron bacteria. Their metabolic activities will release organic acids and other corrosive products. Growth and reproduction in storage tanks and aircraft fuel tanks will cause oil Tank corrosion can corrode and damage the walls of the oil tank and oil tank, causing serious failures such as oil leakage from the oil tank and th...

Claims

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Application Information

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IPC IPC(8): C12Q1/02
CPCC12Q1/02C12Q2304/24
Inventor 王恒伟
Owner ZHEJIANG OCEAN UNIV
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