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Complex microbial agent capable of efficiently degrading polycyclic aromatic hydrocarbons and application of complex microbial agent

A polycyclic aromatic hydrocarbon and compound bacterial agent technology, applied in the field of environmental microorganisms, can solve the problems of slow growth, low growth and slow degradation rate of mycobacteria, achieve strong application prospects and promotion value, improve degradation effect, and fermentation time. short effect

Active Publication Date: 2019-02-22
北京市科学技术研究院资源环境研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Analyzing the existing reports on the degradation of PAHs, the occurrence of mycobacteria is relatively high, mainly because it has a strong ring-opening ability for high-ring PAHs and can quickly complete the first step of decomposition. Self-growth and metabolic characteristics: (1) Mycobacteria grow slowly, usually need a growth period of 1 to 2 weeks, and the growth amount is small, which brings inconvenience and high cost to the actual on-site repair; (2) The degradation of 16 PAHs by a single mycobacterium is relatively poor in broad spectrum, and the degradation rate is slow; (3) there is a lack of co-metabolizing strains that cooperate with mycobacteria to increase the degradation rate

Method used

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  • Complex microbial agent capable of efficiently degrading polycyclic aromatic hydrocarbons and application of complex microbial agent
  • Complex microbial agent capable of efficiently degrading polycyclic aromatic hydrocarbons and application of complex microbial agent
  • Complex microbial agent capable of efficiently degrading polycyclic aromatic hydrocarbons and application of complex microbial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Using PCR-DGGE method to screen degrading strains and synergistic strains

[0032] 1.1 Isolation and cultivation of strains: including separation, identification and cultivation of strains

[0033] Two culture media are used for the culture of bacteria, namely bacterial culture medium I and II:

[0034] Bacterial culture medium I: 2.5 g ammonium sulfate, 0.28 mg ferrous sulfate, 1.5 g disodium hydrogen phosphate, 1.36 g potassium dihydrogen phosphate, 0.25 g magnesium sulfate, 10.7 mg calcium chloride, 0.5 g sodium chloride, ferric chloride 0.004 g, 1 mL trace metal solution, 1 mL vitamin complex solution, add water to 1000 mL and control pH at 7.2-7.4, shake well and use as liquid medium for later use, if it is solid medium, add 1.5% agar powder. The composition of the trace metal liquid is: CoCl 2 ·6H 2 0 35 mg, CuCl 2 0. 20mg, H 3 BO 3 6.0 mg, MnCl 2 4H 2 O 25 mg, Na 2 MoO 4 2H 2 O 3.0 mg, NiCl 2 2H 2 O 2.0 mg, ZnCl 2 2.5 mg, add water to 1...

Embodiment 2

[0061] Example 2 The degradation effects of three kinds of single bacteria on pyrene respectively

[0062] Pyrene to be degraded (final concentration 200ppm) was used as the only carbon source. In the liquid containing bacterial culture medium I, three kinds of single bacteria cultured in equal amounts were respectively added. , 10d to extract and detect by GC-MS; by figure 2 It can be seen that mycobacteria have a strong ability to decompose pyrene, and the degradation rate of the pyrene solution with a final concentration of 200ppm is 77.1% on the 10th day, while the decomposition ability of single microbacteria and Acinetobacter is very weak to pyrene. The 10-day degradation rates were 18.1% and 13.2%, respectively.

Embodiment 3

[0063] Example 3 Degradation effect of single mycobacterium and three kinds of mixed bacteria on pyrene

[0064]Pyrene to be degraded (final concentration 200ppm) was used as the only carbon source, and cultured single mycobacterium and three kinds of mixed bacteria were respectively added to the liquid containing bacterial culture medium I. 6, 8, and 10d were extracted and detected by GC-MS; by image 3 It can be seen that mycobacteria have a strong ability to decompose pyrene, and the degradation rate of the pyrene solution with a final concentration of 200ppm is 77.1% on the 10th day. Three kinds of mixed bacteria (mycobacteria: microbacteria: Acinetobacter=2; 1; 1) The degradation ability was further enhanced, and the degradation rate of pyrene by the mixed bacteria was detected to be 93.2% on the 10th day. Therefore, it can be concluded that Microbacteria and Acinetobacter have a synergistic strengthening effect, and the application effect of the mixed bacteria is better....

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Abstract

The invention provides a complex microbial agent capable of efficiently degrading polycyclic aromatic hydrocarbons. The active ingredients include Mycobacterium sp. having a collection number of CGMCCNo. 6531, Acinetobacter sp. having a collection number of CGMCC No. 6532, and Microbacterium sp. having a collection number of CGMCC No. 6530. The complex microbial agent can take the polycyclic aromatic hydrocarbons, such as fluorine, naphthalene, anthracene, acenaphthene, acenaphthylene, dibenzothiophen, carbazole, chrysene, phenanthrene, pyrene, fluoranthene, benzofluoranthrene, benzanthraceneor benzopyrene as the unique carbon source. The complex microbial agent disclosed by the invention is capable of obviously improving the degradation effect of single colony on organic contaminated soil, the fermentation time of three strains is short, the effect is obvious, and the cost is low. Therefore, the complex microbial agent has strong application prospects and popularization value on polycyclic aromatic hydrocarbon heavy contaminated soil and water.

Description

technical field [0001] The invention belongs to the technical field of environmental microorganisms, and in particular relates to a composite microbial agent for efficiently degrading polycyclic aromatic hydrocarbons and its application. Background technique [0002] Polycyclic aromatic hydrocarbons (PAHs) are a class of environmental pollutants that can persist in the environment for a long time and have strong carcinogenic, teratogenic and mutagenic properties. Such substances are generally poorly soluble in water, have high octanol-water partition coefficients, and low vapor pressures. Under natural conditions, polycyclic aromatic hydrocarbons are very stable and difficult to be biodegraded and utilized. With the increase of the number of benzene rings, the fat-solubility of polycyclic aromatic hydrocarbons increases, and the water-solubility decreases. The longer they exist in the environment, the higher the genotoxicity. [0003] Farmland pollution mainly comes from s...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/32C12R1/07
CPCC12N1/20C02F3/34B09C1/10C12R2001/32C12N1/205C12R2001/07Y02W10/10
Inventor 金京华杨艳程言君高振
Owner 北京市科学技术研究院资源环境研究所
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