Culture method of ganoderma sinensis strains and ganoderma sinensis liquid fermentation method

A cultivation method and strain technology, which are applied in the cultivation field of Zizhi strains, can solve the problem of low yield of Zizhi polysaccharides and the like

Active Publication Date: 2019-02-22
BEIJING BRILLIANCE BIOCHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the defect in the prior art of cultivating the mycelium of Zizhi mycelium to obtain the low yield of polysa

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] 1. Cultivation of Zizhi strains in inclined test tubes:

[0047] Preparation of maple water extraction solution: mix maple branches (dry weight) with deionized water at a ratio of 7:100, boil for 20 minutes, and filter through gauze to obtain maple water extraction solution.

[0048] PDA medium (g / L): Potato 250g, add 500ml deionized water and boil for 30min, filter with gauze to get the filtrate; add glucose 30g, KH 2 PO 4 2g, MgSO 4 ·7H 2 O 0.8g, VB 1 0.01g, 15g of agar, add the maple water extract solution prepared above to make up to 1L.

[0049] Bacteria culture stage: Dig a piece (1*1cm) of bacteria lawn from the storage test tube of Zizhi bacteria (China Agricultural Microorganism Culture Collection Management Center, preservation number: ACCC50045) and inoculate it in solid PDA medium for activation and rejuvenation. After inoculating the strains, culture them in an incubator at 24° C. for 4 days to obtain an activated strain of Zizhi that grows vigorousl...

Embodiment 2

[0058] 1. Cultivation of Zizhi strains in inclined test tubes:

[0059] Preparation of maple water extraction solution: mix maple branches (dry weight) with deionized water at a ratio of 5:100, boil for 50 min, and filter through gauze to obtain maple water extraction solution.

[0060] PDA medium (g / L): Potato 150g, add 500ml deionized water and boil for 30min, filter with gauze to get the filtrate; add glucose 50g, KH 2 PO 4 5g, MgSO 4 ·7H 2 O 0.5g, VB 1 0.03g, 30g agar, add the maple water extract solution prepared above, and dilute to 1L.

[0061] Bacteria culture stage: Dig a piece (1*1cm) of bacteria lawn from the storage test tube of Zizhi bacteria (China Agricultural Microorganism Culture Collection Management Center, preservation number: ACCC50045) and inoculate it in solid PDA medium for activation and rejuvenation. After inoculating the strains, culture them in an incubator at 24° C. for 4 days to obtain an activated strain of Zizhi that grows vigorously (ful...

Embodiment 3

[0071] 1. Cultivation of Zizhi strains in inclined test tubes:

[0072] Preparation of maple water extraction solution: mix maple branches (dry weight) with deionized water at a ratio of 7:100, boil for 40 minutes, and filter through gauze to obtain maple water extraction solution.

[0073] PDA medium (g / L): Potato 300g, add 500ml deionized water and boil for 30min, filter with gauze to get the filtrate; add glucose 10g, KH 2 PO 4 3g, MgSO 4 ·7H 2 O 1.5g, VB 1 0.01g, 5g of agar, add the maple water extract solution prepared above, and dilute to 1L.

[0074] Bacteria culture stage: Dig a piece (1*1cm) of bacteria lawn from the storage test tube of Zizhi bacteria (China Agricultural Microorganism Culture Collection Management Center, preservation number: ACCC50045) and inoculate it in solid PDA medium for activation and rejuvenation. After inoculating the strains, culture them in an incubator at 24° C. for 4 days to obtain an activated strain of Zizhi that grows vigorousl...

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Abstract

The invention provides a culture method of ganoderma sinensis strains. According to the culture method, at the test tube culture stage, a test tube culture medium comprises a nitrogen source, a carbonsource, phosphate, sulfate, agar, and a maple water extraction solution. The invention further provides the culture method of the ganoderma sinensis strains and a ganoderma sinensis liquid fermentation method,. s Shake flask seed culture liquid and/or fermentation culture liquid comprises a nitrogen source, a carbon source, phosphate, sulfate and a maple water extraction solution. An inventor ofthe invention accidentally founds that as for the ganoderma sinensis strains wildly breeding on maples, in the artificial culture process, if the a certain concentration of the maple water extractionsolution is added into the culture medium to serve as a nutritional supplement, the growth of ganoderma sinensis and secretion of effective components of the ganoderma sinensis are greatly promoted, for example, the dry weight of hyphae obtained through the same inoculation amount and the same capacity of fermentation tanks can be increased by at least 1.5 times; and the content of obtained ganoderma sinensis polysaccharides obtained in fermentation liquid is increased by at least 1.6 times.

Description

technical field [0001] The invention relates to a method for cultivating Zizhi bacteria strains and a Zizhi liquid fermentation method. Background technique [0002] Zizhi is a fine product of traditional Chinese ganoderma. The active ingredients of Zizhi mainly include Zizhi polysaccharides and triterpenoids (ganoderic acid). The medicinal value of Zizhi polysaccharide mainly includes anti-cancer, hypoglycemic, immune regulation, and promotion of protein and nucleic acid synthesis; the medicinal value of ganoderma acid mainly includes liver protection and detoxification, lowering cholesterol, inhibiting the release of histamine, and inhibiting vascular tension Peptide conversion, etc. [0003] Zizhi is difficult to domesticate and cultivate artificially, so Zizhi products are rarely seen in the market. Studies have found that the mycelium of Zizhi also contains a large amount of active ingredients of Zizhi, such as Zizhi polysaccharide. Therefore, people began to try to ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P19/04C12R1/645
CPCC12N1/14C12P19/04
Inventor 张雷杨淑琴郭秀茹
Owner BEIJING BRILLIANCE BIOCHEM
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