Application of Electric Stimulation to Increase Biomolecular Molecular Energy Generic Technology
A technology of biomolecules and quantum energy, which is applied in the fields of quantum physics, quantum biology and biomedicine, can solve problems such as decline and loss of function, and achieve the effect of easy operation, convenient operation and wide application
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[0029] Example: Glutamate quantum energy level decline and recovery
[0030] I. Testing materials: Adult Kunming mice from Hubei Experimental Animal Public Service Center.
[0031] Second, the test step:
[0032] (1), decline and detection of glutamate quantum level level
[0033] Configuring artificial ceremony (ACSF) containing (in mm) 125NACL, 2.5KCL, 2Cacl2, 1MGCL 2 , 1.25nah 2 PO 4 , 26nahco 3 And 10D-glucose; pH 7.4. Add a quantity of glutamic acid to 100 mL of ACSF, so that the final concentration of glutamic acid is 50 mm.
[0034]The brain tissue of 4 mice was quickly taken with a vibrator slice (thickness 450 μm), starting from the olfactory ball to the end of the cerebellum. Four mice were collected into an ACSF containing 50 mM glutamic acid for 24 hours, from incubation, 95% O. 2 + 5% co 2 The mixed gas is ventilated to ensure that the oxygen metabolic function of brain-based cell cells is normal. After 24 hours, all brains were removed, that is, the cerebrospinal flu...
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