Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Cancer/testis antigen CDCA5 as esophageal squamous cell carcinoma prognostic marker and esophageal squamous cell carcinoma prognostic marker therapeutic target

A prognostic marker, esophageal squamous cell carcinoma technology, applied in recombinant DNA technology, DNA/RNA fragments, determination/examination of microorganisms, etc.

Inactive Publication Date: 2019-02-19
JIANGSU PROVINCE HOSPITAL THE FIRST AFFILIATED HOSPITAL WITH NANJING MEDICAL UNIV
View PDF3 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, no studies have systematically screened ESCC-associated cancer-testis antigens and explored their association with ESCC prognosis

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Cancer/testis antigen CDCA5 as esophageal squamous cell carcinoma prognostic marker and esophageal squamous cell carcinoma prognostic marker therapeutic target
  • Cancer/testis antigen CDCA5 as esophageal squamous cell carcinoma prognostic marker and esophageal squamous cell carcinoma prognostic marker therapeutic target
  • Cancer/testis antigen CDCA5 as esophageal squamous cell carcinoma prognostic marker and esophageal squamous cell carcinoma prognostic marker therapeutic target

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Example 1 Screening method of cancer-testis gene CDCA5

[0065] See figure 1 , Based on the previously published transcriptome chip data of the Chinese Han population, GEO database, number: GSE53625, from the 32080 original probes on the chip, the probes without gene name or transcript ID annotations and in all 119 pairs of tissue samples are removed The detection rate is less than 80% of the probes, and the remaining 25,890 probes, from which the genes encoding the protein and genes that are predominantly expressed in the testis are screened out.

[0066] The specific method is based on the GENCODE v19 database search to screen out the genes that can code for the protein; compare the 1,336 genes we found in the earlier stage that show dominant expression in testicular tissue, and further retain 764 probes, corresponding to 690 dominant testicular genes; , 21 cancer-testis genes are differentially expressed in esophageal cancer and adjacent tissues, that is, FoldChange ≥ 2 &...

Embodiment 2

[0068] Example 2 Tissue biology test

[0069] 118 cases of esophageal squamous cell carcinoma patients with cancer and adjacent tissues were extracted from Jiangsu Provincial People's Hospital. All patients in the group had not received radiotherapy or chemotherapy before surgery and had complete clinical and pathological data and prognostic follow-up data. After embedding in paraffin, all tissue specimens were first stained with hematoxylin and eosin to confirm that the paraffin tissue block was esophageal squamous cell carcinoma or paracancerous tissue, and the immunohistochemical tissue chip method was used to detect the 118 cases of primary esophagus The expression level of CDCA5 protein in squamous cell carcinoma and paracancerous tissues was then sectioned to select representative areas. Cancer cells in the tumor area were more than 80%, and cancer cells in the paracancerous area were 0.

[0070] For each specimen, the cancer tissue or the adjacent tissues are selected twice,...

Embodiment 3

[0084] Example 3 Expression detection of CDCA5 gene

[0085] Human esophageal squamous cell carcinoma cell lines TE1, Eca109, KYSE30, KYSE70, KYSE150, KYSE180, KYSE410, KYESE450, KYSE510 and normal esophageal epithelial cells HET-1a were purchased from Shanghai Jikai Biological Company, and the cells were cultured. The cells are subjected to RNA extraction and PCR detection.

[0086] The primers of the CDCA5 gene were designed by Guangzhou RiboBio, as follows:

[0087] Upstream primer: 5’-AGAAAGTCAGGCGTTCCTACAG -3’;

[0088] Downstream primer: 5'-GGGAGATTCCAGGGAGAGTCAT -3'.

[0089] Use GAPDH mRNA as an internal control, use 2 -ΔCt Method to calculate the target gene expression level. All samples were repeated three times on the LightCycler480 PCR machine.

[0090] See Figure 5 , PCR results showed that compared with normal esophageal epithelial cells, esophageal squamous cell carcinoma Eca109 and KYSE30 cell lines have higher CDCA5 gene expression.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses cancer / testis antigen CDCA5 as an esophageal squamous cell carcinoma prognostic marker and esophageal squamous cell carcinoma prognostic marker therapeutic target. Twenty one cancer-testis genes related to esophageal squamous cell carcinoma are systematically screened out based on the databases, such as GEO, HBM and GTEx, wherein CDCA5 expression is significantly related toesophageal squamous cell carcinoma prognosis; the acting mechanism of CDCA5 gene is further verified and analyzed through tissue biology and cellular functional science. It is discovered herein for the first time that CDCA5 as a cancer-testis gene related to esophageal squamous cell carcinoma has high expression in cancer tissue of an esophageal squamous cell carcinoma patient, is related to pooresophageal squamous cell carcinoma prognosis and can act as a biomarker to predict prognosis for the esophageal squamous cell carcinoma patient; the expression of shRNA knockdown CDCA5 gene can be utilized to evidently inhibit in-vivo and in-vitro progress level of esophageal squamous cell carcinoma, and it is indicated that a CDCA5 antigen can act as a target to treat esophageal squamous cell carcinoma.

Description

Technical field [0001] The invention relates to a cancer-testis antigen CDCA5, specifically the cancer-testis antigen CDCA5, which can be used as a prognostic marker for esophageal squamous cell carcinoma and its therapeutic target, and belongs to the application field of the cancer-testis antigen CDCA5. Background technique [0002] Esophageal cancer is one of the most common malignant tumors. Globally, the incidence of esophageal cancer ranks eighth among all malignant tumors, and the mortality rate ranks sixth. Approximately 450,000 people suffer from the disease every year. Different from Western countries, esophageal squamous cell carcinoma (esophageal squamous cell carcinoma) accounts for more than 95% of all esophageal cancer cases in my country. In the past ten years, the comprehensive treatment of esophageal squamous cell carcinoma has been continuously improved, but its prognosis is still not ideal, and its five-year overall survival rate is only 15%-25%. Therefore, ex...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12Q1/6837C12N15/11
CPCC12Q1/6837C12Q1/6886C12Q2600/118C12Q2600/158
Inventor 许晶吴卫兵于跃李志华陈亮
Owner JIANGSU PROVINCE HOSPITAL THE FIRST AFFILIATED HOSPITAL WITH NANJING MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com